Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20860 and BWI76_RS20865 are separated by 582 nucleotides BWI76_RS20865 and BWI76_RS20870 are separated by 3 nucleotides BWI76_RS20870 and BWI76_RS20875 are separated by 27 nucleotides BWI76_RS20875 and BWI76_RS20880 are separated by 232 nucleotides
BWI76_RS20860: BWI76_RS20860 - aminoglycoside/multidrug transporter subunit AcrD, at 4,360,172 to 4,363,285
_RS20860
BWI76_RS20865: BWI76_RS20865 - arsenate reductase, at 4,363,868 to 4,364,221
_RS20865
BWI76_RS20870: BWI76_RS20870 - succinyl-diaminopimelate desuccinylase, at 4,364,225 to 4,365,352
_RS20870
BWI76_RS20875: BWI76_RS20875 - membrane protein, at 4,365,380 to 4,365,583
_RS20875
BWI76_RS20880: BWI76_RS20880 - esterase, at 4,365,816 to 4,366,514
_RS20880
Position (kb) 4364
4365
4366 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4363.242 kb on - strand at 4363.366 kb on + strand at 4363.379 kb on + strand at 4363.379 kb on + strand at 4363.387 kb on - strand at 4363.449 kb on + strand at 4363.451 kb on + strand at 4363.459 kb on + strand at 4363.531 kb on + strand at 4363.539 kb on - strand at 4363.549 kb on - strand at 4363.658 kb on + strand at 4363.670 kb on + strand at 4363.670 kb on + strand at 4363.670 kb on + strand at 4363.738 kb on + strand at 4363.744 kb on - strand at 4363.859 kb on + strand at 4363.859 kb on + strand at 4363.859 kb on + strand at 4363.859 kb on + strand at 4363.860 kb on + strand at 4363.867 kb on - strand at 4363.867 kb on - strand at 4363.867 kb on - strand at 4363.867 kb on - strand at 4363.867 kb on - strand at 4363.867 kb on - strand at 4363.867 kb on - strand at 4363.867 kb on - strand at 4363.868 kb on + strand at 4363.931 kb on - strand, within BWI76_RS20865 at 4363.987 kb on - strand, within BWI76_RS20865 at 4364.014 kb on + strand, within BWI76_RS20865 at 4364.014 kb on + strand, within BWI76_RS20865 at 4364.016 kb on + strand, within BWI76_RS20865 at 4364.019 kb on + strand, within BWI76_RS20865 at 4364.022 kb on - strand, within BWI76_RS20865 at 4364.027 kb on - strand, within BWI76_RS20865 at 4364.029 kb on + strand, within BWI76_RS20865 at 4364.029 kb on + strand, within BWI76_RS20865 at 4364.029 kb on + strand, within BWI76_RS20865 at 4364.029 kb on + strand, within BWI76_RS20865 at 4364.037 kb on - strand, within BWI76_RS20865 at 4364.037 kb on - strand, within BWI76_RS20865 at 4364.038 kb on - strand, within BWI76_RS20865 at 4364.046 kb on - strand, within BWI76_RS20865 at 4364.046 kb on - strand, within BWI76_RS20865 at 4364.092 kb on + strand, within BWI76_RS20865 at 4364.094 kb on - strand, within BWI76_RS20865 at 4364.100 kb on - strand, within BWI76_RS20865 at 4364.144 kb on + strand, within BWI76_RS20865 at 4364.206 kb on + strand at 4365.337 kb on + strand at 4365.407 kb on + strand, within BWI76_RS20875 at 4366.189 kb on - strand, within BWI76_RS20880
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 4,363,242 - -1.2 4,363,366 + +0.0 4,363,379 + -1.2 4,363,379 + +1.9 4,363,387 - -0.1 4,363,449 + -1.3 4,363,451 + -0.1 4,363,459 + +0.6 4,363,531 + +2.1 4,363,539 - -0.4 4,363,549 - -0.6 4,363,658 + -0.9 4,363,670 + -0.2 4,363,670 + +1.1 4,363,670 + -0.4 4,363,738 + -0.9 4,363,744 - -0.9 4,363,859 + -0.9 4,363,859 + +1.0 4,363,859 + -0.5 4,363,859 + +0.4 4,363,860 + +0.8 4,363,867 - -0.4 4,363,867 - +0.1 4,363,867 - -0.8 4,363,867 - +0.2 4,363,867 - +0.6 4,363,867 - +1.8 4,363,867 - +0.4 4,363,867 - +0.1 4,363,868 + -0.1 4,363,931 - BWI76_RS20865 0.18 -0.2 4,363,987 - BWI76_RS20865 0.34 -0.2 4,364,014 + BWI76_RS20865 0.41 +0.6 4,364,014 + BWI76_RS20865 0.41 -1.8 4,364,016 + BWI76_RS20865 0.42 -1.4 4,364,019 + BWI76_RS20865 0.43 -0.4 4,364,022 - BWI76_RS20865 0.44 +0.2 4,364,027 - BWI76_RS20865 0.45 -0.7 4,364,029 + BWI76_RS20865 0.45 -0.9 4,364,029 + BWI76_RS20865 0.45 -0.3 4,364,029 + BWI76_RS20865 0.45 +0.3 4,364,029 + BWI76_RS20865 0.45 -0.1 4,364,037 - BWI76_RS20865 0.48 -0.5 4,364,037 - BWI76_RS20865 0.48 -0.8 4,364,038 - BWI76_RS20865 0.48 +0.5 4,364,046 - BWI76_RS20865 0.50 -0.8 4,364,046 - BWI76_RS20865 0.50 -0.7 4,364,092 + BWI76_RS20865 0.63 +0.7 4,364,094 - BWI76_RS20865 0.64 +0.0 4,364,100 - BWI76_RS20865 0.66 -0.4 4,364,144 + BWI76_RS20865 0.78 -0.5 4,364,206 + +0.3 4,365,337 + -0.8 4,365,407 + BWI76_RS20875 0.13 +0.4 4,366,189 - BWI76_RS20880 0.53 -0.6
Or see this region's nucleotide sequence