Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27720 and BWI76_RS27725 are separated by 3 nucleotides BWI76_RS27725 and BWI76_RS27730 overlap by 1 nucleotides BWI76_RS27730 and BWI76_RS27735 are separated by 209 nucleotides BWI76_RS27735 and BWI76_RS27740 are separated by 20 nucleotides
BWI76_RS27720: BWI76_RS27720 - carboxylate/amino acid/amine transporter, at 5,753,817 to 5,754,719
_RS27720
BWI76_RS27725: BWI76_RS27725 - hypothetical protein, at 5,754,723 to 5,754,980
_RS27725
BWI76_RS27730: BWI76_RS27730 - ISNCY family transposase, at 5,754,980 to 5,755,933
_RS27730
BWI76_RS27735: BWI76_RS27735 - hypothetical protein, at 5,756,143 to 5,756,436
_RS27735
BWI76_RS27740: BWI76_RS27740 - LacI family transcriptional regulator, at 5,756,457 to 5,757,398
_RS27740
Position (kb)
5754
5755
5756 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8
9 at 5753.992 kb on + strand, within BWI76_RS27720 at 5754.116 kb on + strand, within BWI76_RS27720 at 5754.196 kb on - strand, within BWI76_RS27720 at 5754.288 kb on + strand, within BWI76_RS27720 at 5754.293 kb on + strand, within BWI76_RS27720 at 5754.293 kb on + strand, within BWI76_RS27720 at 5754.296 kb on - strand, within BWI76_RS27720 at 5754.301 kb on - strand, within BWI76_RS27720 at 5754.301 kb on - strand, within BWI76_RS27720 at 5754.457 kb on + strand, within BWI76_RS27720 at 5754.502 kb on - strand, within BWI76_RS27720 at 5754.511 kb on + strand, within BWI76_RS27720 at 5754.519 kb on - strand, within BWI76_RS27720 at 5754.519 kb on - strand, within BWI76_RS27720 at 5754.582 kb on - strand, within BWI76_RS27720 at 5754.602 kb on - strand, within BWI76_RS27720 at 5754.625 kb on + strand, within BWI76_RS27720 at 5754.869 kb on + strand at 5754.898 kb on + strand at 5754.963 kb on + strand at 5755.016 kb on - strand at 5755.039 kb on + strand at 5755.231 kb on + strand, within BWI76_RS27730 at 5755.239 kb on - strand, within BWI76_RS27730 at 5755.290 kb on - strand, within BWI76_RS27730 at 5755.295 kb on - strand, within BWI76_RS27730 at 5755.315 kb on + strand, within BWI76_RS27730 at 5755.434 kb on - strand, within BWI76_RS27730 at 5755.539 kb on + strand, within BWI76_RS27730 at 5755.568 kb on - strand, within BWI76_RS27730 at 5755.671 kb on + strand, within BWI76_RS27730 at 5755.676 kb on + strand, within BWI76_RS27730 at 5755.684 kb on - strand, within BWI76_RS27730 at 5755.701 kb on + strand, within BWI76_RS27730 at 5755.741 kb on - strand, within BWI76_RS27730 at 5755.771 kb on + strand, within BWI76_RS27730 at 5755.789 kb on - strand, within BWI76_RS27730 at 5755.807 kb on - strand, within BWI76_RS27730 at 5755.809 kb on + strand, within BWI76_RS27730 at 5755.875 kb on + strand at 5755.993 kb on + strand at 5756.050 kb on + strand at 5756.180 kb on + strand, within BWI76_RS27735 at 5756.190 kb on + strand, within BWI76_RS27735 at 5756.207 kb on - strand, within BWI76_RS27735 at 5756.345 kb on - strand, within BWI76_RS27735 at 5756.420 kb on - strand at 5756.504 kb on + strand at 5756.597 kb on + strand, within BWI76_RS27740 at 5756.597 kb on + strand, within BWI76_RS27740 at 5756.597 kb on + strand, within BWI76_RS27740 at 5756.605 kb on - strand, within BWI76_RS27740 at 5756.605 kb on - strand, within BWI76_RS27740 at 5756.702 kb on + strand, within BWI76_RS27740 at 5756.728 kb on + strand, within BWI76_RS27740 at 5756.873 kb on - strand, within BWI76_RS27740 at 5756.897 kb on + strand, within BWI76_RS27740 at 5756.897 kb on + strand, within BWI76_RS27740
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,753,992 + BWI76_RS27720 0.19 -2.6 5,754,116 + BWI76_RS27720 0.33 -0.3 5,754,196 - BWI76_RS27720 0.42 -2.4 5,754,288 + BWI76_RS27720 0.52 -0.8 5,754,293 + BWI76_RS27720 0.53 +2.8 5,754,293 + BWI76_RS27720 0.53 -1.8 5,754,296 - BWI76_RS27720 0.53 +1.1 5,754,301 - BWI76_RS27720 0.54 +0.4 5,754,301 - BWI76_RS27720 0.54 +0.8 5,754,457 + BWI76_RS27720 0.71 +2.1 5,754,502 - BWI76_RS27720 0.76 +1.0 5,754,511 + BWI76_RS27720 0.77 -0.2 5,754,519 - BWI76_RS27720 0.78 -0.6 5,754,519 - BWI76_RS27720 0.78 -0.7 5,754,582 - BWI76_RS27720 0.85 -0.4 5,754,602 - BWI76_RS27720 0.87 +0.8 5,754,625 + BWI76_RS27720 0.89 -0.2 5,754,869 + -0.4 5,754,898 + +1.8 5,754,963 + -2.2 5,755,016 - -1.6 5,755,039 + +1.4 5,755,231 + BWI76_RS27730 0.26 -3.3 5,755,239 - BWI76_RS27730 0.27 -0.8 5,755,290 - BWI76_RS27730 0.32 -2.8 5,755,295 - BWI76_RS27730 0.33 +2.2 5,755,315 + BWI76_RS27730 0.35 +2.3 5,755,434 - BWI76_RS27730 0.48 -0.4 5,755,539 + BWI76_RS27730 0.59 -1.0 5,755,568 - BWI76_RS27730 0.62 +2.0 5,755,671 + BWI76_RS27730 0.72 -1.0 5,755,676 + BWI76_RS27730 0.73 +9.7 5,755,684 - BWI76_RS27730 0.74 -0.2 5,755,701 + BWI76_RS27730 0.76 -0.3 5,755,741 - BWI76_RS27730 0.80 +0.3 5,755,771 + BWI76_RS27730 0.83 +0.6 5,755,789 - BWI76_RS27730 0.85 +4.6 5,755,807 - BWI76_RS27730 0.87 -0.9 5,755,809 + BWI76_RS27730 0.87 -1.1 5,755,875 + -0.9 5,755,993 + -1.0 5,756,050 + +0.6 5,756,180 + BWI76_RS27735 0.13 -2.6 5,756,190 + BWI76_RS27735 0.16 +0.3 5,756,207 - BWI76_RS27735 0.22 -0.7 5,756,345 - BWI76_RS27735 0.69 -0.3 5,756,420 - +0.2 5,756,504 + -1.4 5,756,597 + BWI76_RS27740 0.15 -0.2 5,756,597 + BWI76_RS27740 0.15 -1.6 5,756,597 + BWI76_RS27740 0.15 -2.0 5,756,605 - BWI76_RS27740 0.16 -0.2 5,756,605 - BWI76_RS27740 0.16 -1.2 5,756,702 + BWI76_RS27740 0.26 -0.8 5,756,728 + BWI76_RS27740 0.29 -0.6 5,756,873 - BWI76_RS27740 0.44 +0.3 5,756,897 + BWI76_RS27740 0.47 -0.6 5,756,897 + BWI76_RS27740 0.47 +0.0
Or see this region's nucleotide sequence