Experiment: KLEB20 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12045 and BWI76_RS12050 overlap by 8 nucleotides BWI76_RS12050 and BWI76_RS12055 are separated by 34 nucleotides BWI76_RS12055 and BWI76_RS12060 are separated by 13 nucleotides
BWI76_RS12045: BWI76_RS12045 - hypothetical protein, at 2,531,971 to 2,532,690
_RS12045
BWI76_RS12050: BWI76_RS12050 - hypothetical protein, at 2,532,683 to 2,533,036
_RS12050
BWI76_RS12055: BWI76_RS12055 - DNA single-strand annealing protein RecT-like protein, at 2,533,071 to 2,534,180
_RS12055
BWI76_RS12060: BWI76_RS12060 - putative exodeoxyribonuclease VIII, at 2,534,194 to 2,536,983
_RS12060
Position (kb)
2533
2534
2535 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2532.114 kb on - strand, within BWI76_RS12045 at 2532.233 kb on + strand, within BWI76_RS12045 at 2532.627 kb on + strand at 2532.627 kb on + strand at 2532.642 kb on + strand at 2532.645 kb on - strand at 2532.869 kb on + strand, within BWI76_RS12050 at 2532.877 kb on - strand, within BWI76_RS12050 at 2532.937 kb on + strand, within BWI76_RS12050 at 2532.956 kb on - strand at 2532.989 kb on - strand, within BWI76_RS12050 at 2533.106 kb on - strand at 2533.276 kb on + strand, within BWI76_RS12055 at 2533.531 kb on - strand, within BWI76_RS12055 at 2533.662 kb on - strand, within BWI76_RS12055 at 2533.933 kb on + strand, within BWI76_RS12055 at 2534.271 kb on + strand at 2534.446 kb on + strand at 2534.641 kb on + strand, within BWI76_RS12060 at 2534.723 kb on + strand, within BWI76_RS12060 at 2534.906 kb on - strand, within BWI76_RS12060 at 2534.995 kb on + strand, within BWI76_RS12060
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1 remove 2,532,114 - BWI76_RS12045 0.20 +0.2 2,532,233 + BWI76_RS12045 0.36 +1.2 2,532,627 + +0.8 2,532,627 + +0.2 2,532,642 + +0.2 2,532,645 - +0.8 2,532,869 + BWI76_RS12050 0.53 +1.0 2,532,877 - BWI76_RS12050 0.55 -0.8 2,532,937 + BWI76_RS12050 0.72 -0.8 2,532,956 - +0.9 2,532,989 - BWI76_RS12050 0.86 -2.3 2,533,106 - +0.3 2,533,276 + BWI76_RS12055 0.18 -0.4 2,533,531 - BWI76_RS12055 0.41 -3.0 2,533,662 - BWI76_RS12055 0.53 -2.4 2,533,933 + BWI76_RS12055 0.78 -0.2 2,534,271 + +0.5 2,534,446 + +0.8 2,534,641 + BWI76_RS12060 0.16 +0.6 2,534,723 + BWI76_RS12060 0.19 +0.5 2,534,906 - BWI76_RS12060 0.26 +0.4 2,534,995 + BWI76_RS12060 0.29 -0.7
Or see this region's nucleotide sequence