Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21400 and BWI76_RS21405 are separated by 93 nucleotides BWI76_RS21405 and BWI76_RS21410 are separated by 39 nucleotides BWI76_RS21410 and BWI76_RS21415 are separated by 2 nucleotides
BWI76_RS21400: BWI76_RS21400 - protein lysine acetyltransferase, at 4,478,891 to 4,481,569
_RS21400
BWI76_RS21405: BWI76_RS21405 - phosphatidylserine synthase, at 4,481,663 to 4,483,018
_RS21405
BWI76_RS21410: BWI76_RS21410 - lipoprotein, at 4,483,058 to 4,483,384
_RS21410
BWI76_RS21415: BWI76_RS21415 - MFS transporter, at 4,483,387 to 4,484,685
_RS21415
Position (kb) 4481
4482
4483
4484 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4480.845 kb on + strand, within BWI76_RS21400 at 4480.930 kb on - strand, within BWI76_RS21400 at 4481.019 kb on + strand, within BWI76_RS21400 at 4481.022 kb on - strand, within BWI76_RS21400 at 4481.086 kb on + strand, within BWI76_RS21400 at 4481.112 kb on - strand, within BWI76_RS21400 at 4481.470 kb on + strand at 4483.122 kb on - strand, within BWI76_RS21410 at 4483.123 kb on + strand, within BWI76_RS21410 at 4483.242 kb on - strand, within BWI76_RS21410 at 4483.385 kb on + strand at 4483.574 kb on + strand, within BWI76_RS21415 at 4483.647 kb on + strand, within BWI76_RS21415 at 4483.757 kb on - strand, within BWI76_RS21415 at 4483.902 kb on + strand, within BWI76_RS21415 at 4483.910 kb on - strand, within BWI76_RS21415 at 4483.976 kb on - strand, within BWI76_RS21415
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 4,480,845 + BWI76_RS21400 0.73 +0.9 4,480,930 - BWI76_RS21400 0.76 -1.9 4,481,019 + BWI76_RS21400 0.79 -0.3 4,481,022 - BWI76_RS21400 0.80 +0.8 4,481,086 + BWI76_RS21400 0.82 +0.6 4,481,112 - BWI76_RS21400 0.83 -0.1 4,481,470 + +2.3 4,483,122 - BWI76_RS21410 0.20 -0.9 4,483,123 + BWI76_RS21410 0.20 +1.2 4,483,242 - BWI76_RS21410 0.56 -1.1 4,483,385 + -1.9 4,483,574 + BWI76_RS21415 0.14 +1.3 4,483,647 + BWI76_RS21415 0.20 +0.6 4,483,757 - BWI76_RS21415 0.28 +0.3 4,483,902 + BWI76_RS21415 0.40 -0.6 4,483,910 - BWI76_RS21415 0.40 -3.6 4,483,976 - BWI76_RS21415 0.45 -0.1
Or see this region's nucleotide sequence