Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11455 and BWI76_RS11460 overlap by 17 nucleotides BWI76_RS11460 and BWI76_RS11465 are separated by 152 nucleotides BWI76_RS11465 and BWI76_RS11470 are separated by 27 nucleotides
BWI76_RS11455: BWI76_RS11455 - AraC family transcriptional regulator, at 2,410,967 to 2,411,758
_RS11455
BWI76_RS11460: BWI76_RS11460 - DUF441 family protein, at 2,411,742 to 2,412,188
_RS11460
BWI76_RS11465: BWI76_RS11465 - hypothetical protein, at 2,412,341 to 2,412,841
_RS11465
BWI76_RS11470: BWI76_RS11470 - sensor domain-containing diguanylate cyclase, at 2,412,869 to 2,414,371
_RS11470
Position (kb)
2412
2413 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2411.569 kb on - strand, within BWI76_RS11455 at 2411.656 kb on - strand, within BWI76_RS11455 at 2411.861 kb on + strand, within BWI76_RS11460 at 2412.074 kb on + strand, within BWI76_RS11460 at 2412.164 kb on - strand at 2412.181 kb on - strand at 2412.197 kb on - strand at 2412.197 kb on - strand at 2412.367 kb on + strand at 2412.558 kb on + strand, within BWI76_RS11465 at 2412.680 kb on + strand, within BWI76_RS11465 at 2412.696 kb on + strand, within BWI76_RS11465 at 2412.773 kb on - strand, within BWI76_RS11465 at 2412.980 kb on + strand at 2413.026 kb on + strand, within BWI76_RS11470 at 2413.041 kb on - strand, within BWI76_RS11470 at 2413.125 kb on - strand at 2413.438 kb on + strand, within BWI76_RS11470 at 2413.438 kb on + strand, within BWI76_RS11470 at 2413.470 kb on + strand, within BWI76_RS11470 at 2413.799 kb on + strand, within BWI76_RS11470 at 2413.799 kb on + strand, within BWI76_RS11470 at 2413.799 kb on + strand at 2413.807 kb on - strand, within BWI76_RS11470 at 2413.814 kb on - strand, within BWI76_RS11470
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 2,411,569 - BWI76_RS11455 0.76 -1.4 2,411,656 - BWI76_RS11455 0.87 -0.2 2,411,861 + BWI76_RS11460 0.27 +1.8 2,412,074 + BWI76_RS11460 0.74 +0.4 2,412,164 - +0.2 2,412,181 - -3.1 2,412,197 - +0.5 2,412,197 - +1.6 2,412,367 + -2.7 2,412,558 + BWI76_RS11465 0.43 -2.1 2,412,680 + BWI76_RS11465 0.68 -3.3 2,412,696 + BWI76_RS11465 0.71 -1.6 2,412,773 - BWI76_RS11465 0.86 -0.4 2,412,980 + -1.6 2,413,026 + BWI76_RS11470 0.10 -1.9 2,413,041 - BWI76_RS11470 0.11 +0.8 2,413,125 - +2.1 2,413,438 + BWI76_RS11470 0.38 -0.2 2,413,438 + BWI76_RS11470 0.38 -2.7 2,413,470 + BWI76_RS11470 0.40 -1.9 2,413,799 + BWI76_RS11470 0.62 -3.5 2,413,799 + BWI76_RS11470 0.62 -1.9 2,413,799 + +3.7 2,413,807 - BWI76_RS11470 0.62 -0.5 2,413,814 - BWI76_RS11470 0.63 +0.3
Or see this region's nucleotide sequence