Experiment: KLEB16 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00165 and BWI76_RS00170 are separated by 182 nucleotides BWI76_RS00170 and BWI76_RS00175 are separated by 345 nucleotides
BWI76_RS00165: BWI76_RS00165 - glutamine--fructose-6-phosphate aminotransferase, at 32,128 to 33,957
_RS00165
BWI76_RS00170: BWI76_RS00170 - bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase, at 34,140 to 35,510
_RS00170
BWI76_RS00175: BWI76_RS00175 - pectate disaccharide-lyase, at 35,856 to 38,027
_RS00175
Position (kb) 34
35
36 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 33.989 kb on - strand at 34.081 kb on + strand at 34.081 kb on + strand at 34.089 kb on - strand at 34.089 kb on - strand at 34.107 kb on - strand at 35.618 kb on - strand at 35.651 kb on + strand at 35.651 kb on + strand at 35.871 kb on + strand at 35.926 kb on - strand at 35.958 kb on - strand at 36.079 kb on - strand, within BWI76_RS00175 at 36.181 kb on - strand, within BWI76_RS00175 at 36.313 kb on - strand, within BWI76_RS00175
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB16 MOI 1 remove 33,989 - -0.3 34,081 + +1.1 34,081 + -3.8 34,089 - +0.4 34,089 - +0.6 34,107 - -1.7 35,618 - +0.3 35,651 + +3.5 35,651 + +2.4 35,871 + -0.5 35,926 - +0.5 35,958 - -0.3 36,079 - BWI76_RS00175 0.10 -3.1 36,181 - BWI76_RS00175 0.15 +0.0 36,313 - BWI76_RS00175 0.21 +1.0
Or see this region's nucleotide sequence