Strain Fitness in Pseudomonas simiae WCS417 around PS417_16185
Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
| Position | Strand | Gene | LocusTag | Fraction | TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 |
|---|---|---|---|---|---|
| remove | |||||
| 3,547,609 | - | PS417_16180 | 0.42 | +0.8 | |
| 3,547,609 | - | PS417_16180 | 0.42 | -0.4 | |
| 3,547,656 | + | PS417_16180 | 0.46 | -0.1 | |
| 3,548,036 | - | PS417_16180 | 0.78 | +0.0 | |
| 3,548,130 | + | PS417_16180 | 0.86 | -0.4 | |
| 3,548,131 | - | PS417_16180 | 0.86 | +1.7 | |
| 3,548,367 | - | -1.5 | |||
| 3,548,367 | - | -0.1 | |||
| 3,548,368 | + | -0.8 | |||
| 3,548,449 | - | +1.2 | |||
| 3,548,449 | - | +0.3 | |||
| 3,548,490 | - | PS417_16185 | 0.19 | -0.3 | |
| 3,548,494 | - | PS417_16185 | 0.20 | -1.2 | |
| 3,548,506 | - | PS417_16185 | 0.23 | -0.9 | |
| 3,548,509 | - | PS417_16185 | 0.24 | -0.4 | |
| 3,548,834 | + | -0.6 | |||
| 3,548,865 | + | -0.0 | |||
| 3,549,236 | + | PS417_16190 | 0.37 | +0.1 | |
| 3,549,403 | + | PS417_16190 | 0.53 | -0.4 | |
| 3,549,403 | + | PS417_16190 | 0.53 | -0.2 | |
| 3,549,404 | - | PS417_16190 | 0.54 | -1.2 | |
| 3,549,555 | - | PS417_16190 | 0.69 | +0.0 | |
| 3,549,555 | - | PS417_16190 | 0.69 | +0.4 | |
| 3,549,569 | + | PS417_16190 | 0.70 | -0.0 | |
| 3,549,570 | - | PS417_16190 | 0.70 | -1.1 | |
| 3,549,570 | - | PS417_16190 | 0.70 | +0.6 | |
| 3,549,570 | - | PS417_16190 | 0.70 | -0.3 | |
| 3,549,570 | - | PS417_16190 | 0.70 | -0.8 |
Or see this region's nucleotide sequence