Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24400 and BWI76_RS24405 are separated by 169 nucleotides BWI76_RS24405 and BWI76_RS24410 are separated by 5 nucleotides BWI76_RS24410 and BWI76_RS24415 are separated by 46 nucleotides
BWI76_RS24400: BWI76_RS24400 - outer membrane channel protein TolC, at 5,085,527 to 5,086,975
_RS24400
BWI76_RS24405: BWI76_RS24405 - hypothetical protein, at 5,087,145 to 5,087,813
_RS24405
BWI76_RS24410: BWI76_RS24410 - putative glutathione-like synthetase, at 5,087,819 to 5,088,979
_RS24410
BWI76_RS24415: BWI76_RS24415 - dioxygenase, at 5,089,026 to 5,089,817
_RS24415
Position (kb)
5087
5088
5089 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 5086.961 kb on - strand at 5087.005 kb on + strand at 5087.037 kb on - strand at 5087.037 kb on - strand at 5087.092 kb on + strand at 5087.095 kb on - strand at 5087.100 kb on - strand at 5087.323 kb on + strand, within BWI76_RS24405 at 5087.343 kb on + strand, within BWI76_RS24405 at 5087.343 kb on + strand, within BWI76_RS24405 at 5087.351 kb on - strand, within BWI76_RS24405 at 5087.405 kb on - strand, within BWI76_RS24405 at 5087.579 kb on - strand, within BWI76_RS24405 at 5087.644 kb on + strand, within BWI76_RS24405 at 5087.649 kb on + strand, within BWI76_RS24405 at 5087.711 kb on - strand, within BWI76_RS24405 at 5087.768 kb on - strand at 5087.973 kb on + strand, within BWI76_RS24410 at 5087.973 kb on + strand, within BWI76_RS24410 at 5088.138 kb on + strand, within BWI76_RS24410 at 5088.405 kb on - strand, within BWI76_RS24410 at 5088.555 kb on - strand, within BWI76_RS24410 at 5088.642 kb on - strand, within BWI76_RS24410 at 5088.728 kb on + strand, within BWI76_RS24410 at 5088.819 kb on + strand, within BWI76_RS24410 at 5088.827 kb on - strand, within BWI76_RS24410 at 5088.837 kb on - strand, within BWI76_RS24410 at 5088.929 kb on - strand at 5088.987 kb on - strand at 5089.231 kb on + strand, within BWI76_RS24415 at 5089.239 kb on - strand, within BWI76_RS24415 at 5089.264 kb on + strand, within BWI76_RS24415 at 5089.310 kb on + strand, within BWI76_RS24415 at 5089.311 kb on - strand, within BWI76_RS24415 at 5089.311 kb on - strand, within BWI76_RS24415 at 5089.319 kb on - strand, within BWI76_RS24415 at 5089.509 kb on - strand, within BWI76_RS24415 at 5089.509 kb on - strand, within BWI76_RS24415 at 5089.571 kb on + strand, within BWI76_RS24415 at 5089.652 kb on + strand, within BWI76_RS24415 at 5089.652 kb on + strand, within BWI76_RS24415 at 5089.652 kb on + strand, within BWI76_RS24415 at 5089.660 kb on - strand, within BWI76_RS24415 at 5089.660 kb on - strand, within BWI76_RS24415 at 5089.660 kb on - strand, within BWI76_RS24415 at 5089.664 kb on + strand, within BWI76_RS24415 at 5089.671 kb on + strand, within BWI76_RS24415 at 5089.672 kb on - strand, within BWI76_RS24415 at 5089.672 kb on - strand, within BWI76_RS24415 at 5089.672 kb on - strand, within BWI76_RS24415 at 5089.696 kb on + strand, within BWI76_RS24415 at 5089.696 kb on + strand, within BWI76_RS24415 at 5089.696 kb on + strand, within BWI76_RS24415 at 5089.704 kb on - strand, within BWI76_RS24415 at 5089.704 kb on - strand, within BWI76_RS24415 at 5089.747 kb on - strand at 5089.779 kb on - strand at 5089.779 kb on - strand at 5089.793 kb on + strand at 5089.827 kb on - strand at 5089.827 kb on - strand at 5089.827 kb on - strand at 5089.839 kb on - strand at 5089.839 kb on - strand at 5089.848 kb on + strand at 5089.856 kb on - strand at 5089.860 kb on - strand at 5089.883 kb on - strand at 5089.888 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 5,086,961 - +0.3 5,087,005 + -1.7 5,087,037 - -0.4 5,087,037 - +2.7 5,087,092 + +0.2 5,087,095 - +0.9 5,087,100 - -0.6 5,087,323 + BWI76_RS24405 0.27 +1.8 5,087,343 + BWI76_RS24405 0.30 -0.1 5,087,343 + BWI76_RS24405 0.30 +0.3 5,087,351 - BWI76_RS24405 0.31 +1.2 5,087,405 - BWI76_RS24405 0.39 -2.4 5,087,579 - BWI76_RS24405 0.65 -1.4 5,087,644 + BWI76_RS24405 0.75 +0.3 5,087,649 + BWI76_RS24405 0.75 -1.4 5,087,711 - BWI76_RS24405 0.85 +0.7 5,087,768 - -0.5 5,087,973 + BWI76_RS24410 0.13 -1.3 5,087,973 + BWI76_RS24410 0.13 -0.9 5,088,138 + BWI76_RS24410 0.27 -0.3 5,088,405 - BWI76_RS24410 0.50 -2.9 5,088,555 - BWI76_RS24410 0.63 -0.7 5,088,642 - BWI76_RS24410 0.71 -0.5 5,088,728 + BWI76_RS24410 0.78 -0.7 5,088,819 + BWI76_RS24410 0.86 +0.4 5,088,827 - BWI76_RS24410 0.87 -2.6 5,088,837 - BWI76_RS24410 0.88 -0.4 5,088,929 - +1.3 5,088,987 - -1.3 5,089,231 + BWI76_RS24415 0.26 -0.6 5,089,239 - BWI76_RS24415 0.27 +0.4 5,089,264 + BWI76_RS24415 0.30 +1.0 5,089,310 + BWI76_RS24415 0.36 -0.7 5,089,311 - BWI76_RS24415 0.36 -0.3 5,089,311 - BWI76_RS24415 0.36 +0.6 5,089,319 - BWI76_RS24415 0.37 -0.3 5,089,509 - BWI76_RS24415 0.61 +0.8 5,089,509 - BWI76_RS24415 0.61 -0.2 5,089,571 + BWI76_RS24415 0.69 +1.2 5,089,652 + BWI76_RS24415 0.79 +0.4 5,089,652 + BWI76_RS24415 0.79 +1.1 5,089,652 + BWI76_RS24415 0.79 -0.2 5,089,660 - BWI76_RS24415 0.80 -0.1 5,089,660 - BWI76_RS24415 0.80 -1.0 5,089,660 - BWI76_RS24415 0.80 -3.1 5,089,664 + BWI76_RS24415 0.81 +0.1 5,089,671 + BWI76_RS24415 0.81 +4.4 5,089,672 - BWI76_RS24415 0.82 +3.4 5,089,672 - BWI76_RS24415 0.82 +0.2 5,089,672 - BWI76_RS24415 0.82 -1.0 5,089,696 + BWI76_RS24415 0.85 -0.5 5,089,696 + BWI76_RS24415 0.85 -0.5 5,089,696 + BWI76_RS24415 0.85 +0.6 5,089,704 - BWI76_RS24415 0.86 +0.8 5,089,704 - BWI76_RS24415 0.86 +0.0 5,089,747 - -0.1 5,089,779 - -0.8 5,089,779 - +0.2 5,089,793 + -0.4 5,089,827 - -1.0 5,089,827 - -0.1 5,089,827 - +0.4 5,089,839 - +2.8 5,089,839 - +1.2 5,089,848 + -0.3 5,089,856 - -1.0 5,089,860 - +0.8 5,089,883 - -0.6 5,089,888 - -1.8
Or see this region's nucleotide sequence