Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18395 and BWI76_RS18400 are separated by 107 nucleotides BWI76_RS18400 and BWI76_RS18405 are separated by 75 nucleotides BWI76_RS18405 and BWI76_RS18410 are separated by 183 nucleotides
BWI76_RS18395: BWI76_RS18395 - diguanylate cyclase, at 3,817,280 to 3,818,947
_RS18395
BWI76_RS18400: BWI76_RS18400 - DUF2158 domain-containing protein, at 3,819,055 to 3,819,234
_RS18400
BWI76_RS18405: BWI76_RS18405 - hypothetical protein, at 3,819,310 to 3,820,221
_RS18405
BWI76_RS18410: BWI76_RS18410 - drug/metabolite exporter YedA, at 3,820,405 to 3,821,316
_RS18410
Position (kb)
3819
3820
3821 Strain fitness (log2 ratio)
-2
-1
0
1 at 3818.316 kb on + strand, within BWI76_RS18395 at 3818.324 kb on - strand, within BWI76_RS18395 at 3818.508 kb on + strand, within BWI76_RS18395 at 3818.672 kb on - strand, within BWI76_RS18395 at 3818.672 kb on - strand, within BWI76_RS18395 at 3818.808 kb on - strand at 3819.140 kb on - strand, within BWI76_RS18400 at 3819.409 kb on - strand, within BWI76_RS18405 at 3819.476 kb on - strand, within BWI76_RS18405 at 3819.476 kb on - strand, within BWI76_RS18405 at 3819.476 kb on - strand, within BWI76_RS18405 at 3819.783 kb on + strand, within BWI76_RS18405 at 3820.064 kb on + strand, within BWI76_RS18405 at 3820.089 kb on + strand, within BWI76_RS18405 at 3820.089 kb on + strand, within BWI76_RS18405 at 3820.117 kb on + strand, within BWI76_RS18405 at 3820.198 kb on + strand at 3820.206 kb on - strand at 3820.390 kb on - strand at 3820.679 kb on + strand, within BWI76_RS18410 at 3820.848 kb on + strand, within BWI76_RS18410 at 3820.848 kb on + strand, within BWI76_RS18410 at 3820.848 kb on + strand, within BWI76_RS18410 at 3820.848 kb on + strand, within BWI76_RS18410 at 3821.203 kb on + strand, within BWI76_RS18410
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 3,818,316 + BWI76_RS18395 0.62 -0.5 3,818,324 - BWI76_RS18395 0.63 +0.8 3,818,508 + BWI76_RS18395 0.74 +0.6 3,818,672 - BWI76_RS18395 0.83 -0.6 3,818,672 - BWI76_RS18395 0.83 +0.0 3,818,808 - -2.1 3,819,140 - BWI76_RS18400 0.47 -0.7 3,819,409 - BWI76_RS18405 0.11 +0.0 3,819,476 - BWI76_RS18405 0.18 +0.4 3,819,476 - BWI76_RS18405 0.18 -0.9 3,819,476 - BWI76_RS18405 0.18 -0.1 3,819,783 + BWI76_RS18405 0.52 -0.5 3,820,064 + BWI76_RS18405 0.83 -2.1 3,820,089 + BWI76_RS18405 0.85 -1.1 3,820,089 + BWI76_RS18405 0.85 +0.2 3,820,117 + BWI76_RS18405 0.88 -1.9 3,820,198 + +1.3 3,820,206 - -0.0 3,820,390 - +0.5 3,820,679 + BWI76_RS18410 0.30 -1.9 3,820,848 + BWI76_RS18410 0.49 +1.0 3,820,848 + BWI76_RS18410 0.49 +0.7 3,820,848 + BWI76_RS18410 0.49 -1.2 3,820,848 + BWI76_RS18410 0.49 -1.0 3,821,203 + BWI76_RS18410 0.88 +1.3
Or see this region's nucleotide sequence