Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nleL and MCAODC_22510 are separated by 19 nucleotides MCAODC_22510 and MCAODC_22515 are separated by 12 nucleotides MCAODC_22515 and rri1 overlap by 4 nucleotides rri1 and MCAODC_22525 are separated by 53 nucleotides
MCAODC_22505: nleL - type III secretion system effector E3 ubiquitin ligase NleL, at 1,261,635 to 1,263,983
nleL
MCAODC_22510: MCAODC_22510 - hypothetical protein, at 1,264,003 to 1,264,092
_22510
MCAODC_22515: MCAODC_22515 - tail assembly protein, at 1,264,105 to 1,264,290
_22515
MCAODC_22520: rri1 - phage tail protein, at 1,264,287 to 1,265,024
rri1
MCAODC_22525: MCAODC_22525 - phage repressor protein/antirepressor Ant, at 1,265,078 to 1,265,956
_22525
Position (kb)
1264
1265 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1263.108 kb on - strand, within nleL at 1263.573 kb on + strand, within nleL at 1263.669 kb on - strand, within nleL at 1263.669 kb on - strand, within nleL at 1263.813 kb on - strand at 1263.943 kb on - strand at 1263.943 kb on - strand at 1264.017 kb on + strand, within MCAODC_22510 at 1264.114 kb on + strand at 1264.128 kb on + strand, within MCAODC_22515 at 1264.139 kb on + strand, within MCAODC_22515 at 1264.201 kb on - strand, within MCAODC_22515 at 1264.574 kb on - strand, within rri1 at 1264.646 kb on - strand, within rri1 at 1264.667 kb on - strand, within rri1 at 1265.007 kb on + strand at 1265.009 kb on + strand at 1265.010 kb on - strand at 1265.010 kb on - strand at 1265.010 kb on - strand at 1265.016 kb on - strand at 1265.017 kb on + strand at 1265.017 kb on + strand at 1265.018 kb on - strand at 1265.018 kb on - strand at 1265.018 kb on - strand at 1265.083 kb on - strand at 1265.103 kb on - strand at 1265.116 kb on + strand at 1265.116 kb on + strand at 1265.185 kb on + strand, within MCAODC_22525 at 1265.212 kb on + strand, within MCAODC_22525 at 1265.217 kb on + strand, within MCAODC_22525 at 1265.217 kb on + strand, within MCAODC_22525 at 1265.218 kb on - strand, within MCAODC_22525 at 1265.236 kb on - strand, within MCAODC_22525 at 1265.241 kb on + strand, within MCAODC_22525
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 1,263,108 - nleL MCAODC_22505 0.63 -1.7 1,263,573 + nleL MCAODC_22505 0.83 -1.1 1,263,669 - nleL MCAODC_22505 0.87 -1.2 1,263,669 - nleL MCAODC_22505 0.87 +2.2 1,263,813 - -3.2 1,263,943 - +0.4 1,263,943 - -0.5 1,264,017 + MCAODC_22510 0.16 +2.0 1,264,114 + -2.1 1,264,128 + MCAODC_22515 0.12 +0.3 1,264,139 + MCAODC_22515 0.18 +0.3 1,264,201 - MCAODC_22515 0.52 +0.8 1,264,574 - rri1 MCAODC_22520 0.39 -0.9 1,264,646 - rri1 MCAODC_22520 0.49 -1.4 1,264,667 - rri1 MCAODC_22520 0.51 +0.3 1,265,007 + -4.3 1,265,009 + -0.3 1,265,010 - +0.4 1,265,010 - -0.5 1,265,010 - -3.2 1,265,016 - +1.8 1,265,017 + +1.2 1,265,017 + +1.2 1,265,018 - -0.8 1,265,018 - +2.1 1,265,018 - -1.8 1,265,083 - +1.7 1,265,103 - -2.0 1,265,116 + -1.6 1,265,116 + -0.0 1,265,185 + MCAODC_22525 0.12 +0.4 1,265,212 + MCAODC_22525 0.15 +0.7 1,265,217 + MCAODC_22525 0.16 +0.5 1,265,217 + MCAODC_22525 0.16 -0.7 1,265,218 - MCAODC_22525 0.16 -0.3 1,265,236 - MCAODC_22525 0.18 +0.3 1,265,241 + MCAODC_22525 0.19 -0.6
Or see this region's nucleotide sequence