Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metK and speA are separated by 794 nucleotides speA and BNILDI_09760 are separated by 145 nucleotides
BNILDI_09750: metK - methionine adenosyltransferase, at 1,959,753 to 1,960,907
metK
BNILDI_09755: speA - biosynthetic arginine decarboxylase, at 1,961,702 to 1,963,678
speA
BNILDI_09760: BNILDI_09760 - Lipoprotein, at 1,963,824 to 1,964,555
_09760
Position (kb)
1961
1962
1963
1964 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1960.978 kb on - strand at 1961.112 kb on - strand at 1961.158 kb on - strand at 1961.193 kb on + strand at 1961.194 kb on - strand at 1961.212 kb on + strand at 1961.217 kb on - strand at 1961.294 kb on + strand at 1961.295 kb on - strand at 1961.316 kb on + strand at 1961.405 kb on + strand at 1961.406 kb on - strand at 1961.420 kb on + strand at 1961.458 kb on - strand at 1961.491 kb on + strand at 1961.534 kb on + strand at 1961.535 kb on - strand at 1961.556 kb on - strand at 1961.625 kb on + strand at 1961.682 kb on - strand at 1961.687 kb on - strand at 1961.701 kb on + strand at 1961.702 kb on - strand at 1961.754 kb on + strand at 1961.754 kb on + strand at 1961.754 kb on + strand at 1961.755 kb on - strand at 1961.757 kb on + strand at 1961.757 kb on + strand at 1961.812 kb on + strand at 1961.813 kb on - strand at 1961.817 kb on - strand at 1961.821 kb on + strand at 1961.840 kb on - strand at 1961.840 kb on - strand at 1961.843 kb on + strand at 1961.843 kb on + strand at 1961.844 kb on - strand at 1961.846 kb on + strand at 1961.846 kb on + strand at 1961.847 kb on - strand at 1961.847 kb on - strand at 1961.847 kb on - strand at 1961.926 kb on - strand, within speA at 1962.049 kb on + strand, within speA at 1962.085 kb on + strand, within speA at 1962.223 kb on + strand, within speA at 1962.223 kb on + strand, within speA at 1962.223 kb on + strand, within speA at 1962.253 kb on - strand, within speA at 1962.505 kb on - strand, within speA at 1962.505 kb on - strand, within speA at 1962.581 kb on + strand, within speA at 1962.582 kb on - strand, within speA at 1962.651 kb on - strand, within speA at 1962.658 kb on + strand, within speA at 1962.658 kb on + strand, within speA at 1962.705 kb on - strand, within speA at 1962.705 kb on - strand, within speA at 1962.852 kb on - strand, within speA at 1962.853 kb on + strand, within speA at 1962.854 kb on - strand, within speA at 1962.854 kb on - strand, within speA at 1962.992 kb on - strand, within speA at 1963.055 kb on + strand, within speA at 1963.158 kb on - strand, within speA at 1963.279 kb on + strand, within speA at 1963.279 kb on + strand, within speA at 1963.279 kb on + strand, within speA at 1963.279 kb on + strand, within speA at 1963.310 kb on + strand, within speA at 1963.311 kb on - strand, within speA at 1963.312 kb on + strand, within speA at 1963.312 kb on + strand, within speA at 1963.313 kb on - strand, within speA at 1963.313 kb on - strand, within speA at 1963.328 kb on - strand, within speA at 1963.328 kb on - strand, within speA at 1963.355 kb on + strand, within speA at 1963.356 kb on - strand, within speA at 1963.390 kb on + strand, within speA at 1963.424 kb on - strand, within speA at 1963.485 kb on + strand at 1963.486 kb on - strand at 1963.492 kb on - strand at 1963.535 kb on + strand at 1963.539 kb on + strand at 1963.539 kb on + strand at 1963.540 kb on - strand at 1963.564 kb on - strand at 1963.655 kb on + strand at 1963.657 kb on + strand at 1963.657 kb on + strand at 1963.658 kb on - strand at 1963.658 kb on - strand at 1963.899 kb on + strand, within BNILDI_09760 at 1963.899 kb on + strand, within BNILDI_09760 at 1963.899 kb on + strand, within BNILDI_09760 at 1963.900 kb on - strand, within BNILDI_09760 at 1963.905 kb on - strand, within BNILDI_09760 at 1963.970 kb on - strand, within BNILDI_09760 at 1964.020 kb on - strand, within BNILDI_09760 at 1964.131 kb on - strand, within BNILDI_09760 at 1964.158 kb on - strand, within BNILDI_09760 at 1964.160 kb on - strand, within BNILDI_09760 at 1964.209 kb on - strand, within BNILDI_09760 at 1964.227 kb on - strand, within BNILDI_09760 at 1964.299 kb on - strand, within BNILDI_09760 at 1964.299 kb on - strand, within BNILDI_09760 at 1964.301 kb on - strand, within BNILDI_09760 at 1964.348 kb on + strand, within BNILDI_09760 at 1964.354 kb on + strand, within BNILDI_09760 at 1964.355 kb on - strand, within BNILDI_09760 at 1964.519 kb on + strand at 1964.520 kb on - strand at 1964.520 kb on - strand at 1964.553 kb on + strand at 1964.554 kb on - strand at 1964.583 kb on - strand at 1964.583 kb on - strand at 1964.591 kb on - strand at 1964.675 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,960,978 - +0.3 1,961,112 - -0.5 1,961,158 - +1.1 1,961,193 + +0.5 1,961,194 - -3.8 1,961,212 + +0.3 1,961,217 - +2.3 1,961,294 + -1.3 1,961,295 - -0.8 1,961,316 + -1.5 1,961,405 + -1.2 1,961,406 - -2.1 1,961,420 + -0.0 1,961,458 - -0.7 1,961,491 + -1.1 1,961,534 + +0.2 1,961,535 - -0.2 1,961,556 - +1.9 1,961,625 + -2.5 1,961,682 - -2.2 1,961,687 - -0.5 1,961,701 + +3.0 1,961,702 - -1.2 1,961,754 + +0.1 1,961,754 + -0.9 1,961,754 + -0.9 1,961,755 - -0.4 1,961,757 + -1.0 1,961,757 + -1.1 1,961,812 + -0.2 1,961,813 - -2.5 1,961,817 - -0.8 1,961,821 + -3.3 1,961,840 - -1.1 1,961,840 - -4.6 1,961,843 + -0.4 1,961,843 + +2.1 1,961,844 - -3.4 1,961,846 + -0.8 1,961,846 + -2.7 1,961,847 - -0.9 1,961,847 - -2.7 1,961,847 - -3.3 1,961,926 - speA BNILDI_09755 0.11 -4.1 1,962,049 + speA BNILDI_09755 0.18 -1.0 1,962,085 + speA BNILDI_09755 0.19 -1.3 1,962,223 + speA BNILDI_09755 0.26 -2.2 1,962,223 + speA BNILDI_09755 0.26 -0.2 1,962,223 + speA BNILDI_09755 0.26 -0.6 1,962,253 - speA BNILDI_09755 0.28 -0.9 1,962,505 - speA BNILDI_09755 0.41 +0.1 1,962,505 - speA BNILDI_09755 0.41 -2.4 1,962,581 + speA BNILDI_09755 0.44 -0.1 1,962,582 - speA BNILDI_09755 0.45 -1.1 1,962,651 - speA BNILDI_09755 0.48 -0.1 1,962,658 + speA BNILDI_09755 0.48 -0.2 1,962,658 + speA BNILDI_09755 0.48 -0.8 1,962,705 - speA BNILDI_09755 0.51 -1.6 1,962,705 - speA BNILDI_09755 0.51 -3.2 1,962,852 - speA BNILDI_09755 0.58 -2.4 1,962,853 + speA BNILDI_09755 0.58 -1.8 1,962,854 - speA BNILDI_09755 0.58 -1.8 1,962,854 - speA BNILDI_09755 0.58 -0.9 1,962,992 - speA BNILDI_09755 0.65 -1.0 1,963,055 + speA BNILDI_09755 0.68 -1.3 1,963,158 - speA BNILDI_09755 0.74 -3.7 1,963,279 + speA BNILDI_09755 0.80 -4.0 1,963,279 + speA BNILDI_09755 0.80 -1.7 1,963,279 + speA BNILDI_09755 0.80 -0.9 1,963,279 + speA BNILDI_09755 0.80 +1.8 1,963,310 + speA BNILDI_09755 0.81 -3.2 1,963,311 - speA BNILDI_09755 0.81 +0.6 1,963,312 + speA BNILDI_09755 0.81 -0.4 1,963,312 + speA BNILDI_09755 0.81 -2.9 1,963,313 - speA BNILDI_09755 0.81 -1.3 1,963,313 - speA BNILDI_09755 0.81 -1.2 1,963,328 - speA BNILDI_09755 0.82 -0.6 1,963,328 - speA BNILDI_09755 0.82 -0.7 1,963,355 + speA BNILDI_09755 0.84 -1.7 1,963,356 - speA BNILDI_09755 0.84 +0.6 1,963,390 + speA BNILDI_09755 0.85 -4.0 1,963,424 - speA BNILDI_09755 0.87 -1.3 1,963,485 + -1.2 1,963,486 - -0.2 1,963,492 - -0.8 1,963,535 + -0.0 1,963,539 + -1.6 1,963,539 + -0.2 1,963,540 - +0.0 1,963,564 - -2.8 1,963,655 + -0.1 1,963,657 + +0.7 1,963,657 + +0.4 1,963,658 - -3.7 1,963,658 - -3.0 1,963,899 + BNILDI_09760 0.10 -1.4 1,963,899 + BNILDI_09760 0.10 +1.1 1,963,899 + BNILDI_09760 0.10 -2.8 1,963,900 - BNILDI_09760 0.10 +1.4 1,963,905 - BNILDI_09760 0.11 -0.3 1,963,970 - BNILDI_09760 0.20 -0.9 1,964,020 - BNILDI_09760 0.27 +0.3 1,964,131 - BNILDI_09760 0.42 +2.1 1,964,158 - BNILDI_09760 0.46 +0.3 1,964,160 - BNILDI_09760 0.46 -0.3 1,964,209 - BNILDI_09760 0.53 +0.3 1,964,227 - BNILDI_09760 0.55 -1.1 1,964,299 - BNILDI_09760 0.65 -1.2 1,964,299 - BNILDI_09760 0.65 +0.0 1,964,301 - BNILDI_09760 0.65 +0.2 1,964,348 + BNILDI_09760 0.72 +0.8 1,964,354 + BNILDI_09760 0.72 +0.8 1,964,355 - BNILDI_09760 0.73 +0.0 1,964,519 + +0.8 1,964,520 - +1.5 1,964,520 - -0.4 1,964,553 + +0.2 1,964,554 - -0.3 1,964,583 - -0.1 1,964,583 - +0.4 1,964,591 - +0.4 1,964,675 + -0.9
Or see this region's nucleotide sequence