Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoG and nuoH overlap by 4 nucleotides nuoH and nuoI are separated by 14 nucleotides nuoI and nuoJ are separated by 11 nucleotides nuoJ and nuoK overlap by 4 nucleotides nuoK and nuoL overlap by 4 nucleotides
OKFHMN_22865: nuoG - NADH-quinone oxidoreductase subunit NuoG, at 4,448,334 to 4,451,060
nuoG
OKFHMN_22870: nuoH - NADH-quinone oxidoreductase subunit NuoH, at 4,451,057 to 4,452,034
nuoH
OKFHMN_22875: nuoI - NADH-quinone oxidoreductase subunit NuoI, at 4,452,049 to 4,452,591
nuoI
OKFHMN_22880: nuoJ - NADH-quinone oxidoreductase subunit J, at 4,452,603 to 4,453,157
nuoJ
OKFHMN_22885: nuoK - NADH-quinone oxidoreductase subunit NuoK, at 4,453,154 to 4,453,456
nuoK
OKFHMN_22890: nuoL - NADH-quinone oxidoreductase subunit L, at 4,453,453 to 4,455,294
nuoL
Position (kb)
4452
4453 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4451.203 kb on + strand, within nuoH at 4451.264 kb on - strand, within nuoH at 4451.467 kb on + strand, within nuoH at 4451.467 kb on + strand, within nuoH at 4451.467 kb on + strand, within nuoH at 4451.780 kb on + strand, within nuoH at 4452.093 kb on + strand at 4452.094 kb on - strand at 4452.096 kb on + strand at 4452.190 kb on - strand, within nuoI at 4452.422 kb on - strand, within nuoI at 4452.490 kb on + strand, within nuoI at 4452.621 kb on - strand at 4452.621 kb on - strand at 4452.640 kb on + strand at 4452.640 kb on + strand at 4452.641 kb on - strand at 4452.641 kb on - strand at 4452.693 kb on + strand, within nuoJ at 4452.748 kb on - strand, within nuoJ at 4452.995 kb on + strand, within nuoJ at 4452.996 kb on - strand, within nuoJ at 4453.015 kb on + strand, within nuoJ at 4453.038 kb on - strand, within nuoJ at 4453.164 kb on + strand at 4453.337 kb on + strand, within nuoK at 4453.338 kb on - strand, within nuoK at 4453.477 kb on - strand at 4453.583 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,451,203 + nuoH OKFHMN_22870 0.15 -2.7 4,451,264 - nuoH OKFHMN_22870 0.21 -0.4 4,451,467 + nuoH OKFHMN_22870 0.42 -0.5 4,451,467 + nuoH OKFHMN_22870 0.42 -2.4 4,451,467 + nuoH OKFHMN_22870 0.42 -3.0 4,451,780 + nuoH OKFHMN_22870 0.74 -1.1 4,452,093 + +1.6 4,452,094 - +0.7 4,452,096 + +1.1 4,452,190 - nuoI OKFHMN_22875 0.26 -1.7 4,452,422 - nuoI OKFHMN_22875 0.69 -2.7 4,452,490 + nuoI OKFHMN_22875 0.81 -0.3 4,452,621 - -1.5 4,452,621 - -0.3 4,452,640 + -1.2 4,452,640 + -0.2 4,452,641 - -0.3 4,452,641 - +0.8 4,452,693 + nuoJ OKFHMN_22880 0.16 -0.8 4,452,748 - nuoJ OKFHMN_22880 0.26 +0.2 4,452,995 + nuoJ OKFHMN_22880 0.71 -2.3 4,452,996 - nuoJ OKFHMN_22880 0.71 +1.2 4,453,015 + nuoJ OKFHMN_22880 0.74 -3.2 4,453,038 - nuoJ OKFHMN_22880 0.78 +0.1 4,453,164 + -0.3 4,453,337 + nuoK OKFHMN_22885 0.60 +0.0 4,453,338 - nuoK OKFHMN_22885 0.61 +0.8 4,453,477 - +1.3 4,453,583 + -0.5
Or see this region's nucleotide sequence