Strain Fitness in Escherichia coli ECRC100 around OKFHMN_22875

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnuoG and nuoH overlap by 4 nucleotidesnuoH and nuoI are separated by 14 nucleotidesnuoI and nuoJ are separated by 11 nucleotidesnuoJ and nuoK overlap by 4 nucleotidesnuoK and nuoL overlap by 4 nucleotides OKFHMN_22865: nuoG - NADH-quinone oxidoreductase subunit NuoG, at 4,448,334 to 4,451,060 nuoG OKFHMN_22870: nuoH - NADH-quinone oxidoreductase subunit NuoH, at 4,451,057 to 4,452,034 nuoH OKFHMN_22875: nuoI - NADH-quinone oxidoreductase subunit NuoI, at 4,452,049 to 4,452,591 nuoI OKFHMN_22880: nuoJ - NADH-quinone oxidoreductase subunit J, at 4,452,603 to 4,453,157 nuoJ OKFHMN_22885: nuoK - NADH-quinone oxidoreductase subunit NuoK, at 4,453,154 to 4,453,456 nuoK OKFHMN_22890: nuoL - NADH-quinone oxidoreductase subunit L, at 4,453,453 to 4,455,294 nuoL Position (kb) 4452 4453Strain fitness (log2 ratio) -3 -2 -1 0 1at 4451.203 kb on + strand, within nuoHat 4451.264 kb on - strand, within nuoHat 4451.467 kb on + strand, within nuoHat 4451.467 kb on + strand, within nuoHat 4451.467 kb on + strand, within nuoHat 4451.780 kb on + strand, within nuoHat 4452.093 kb on + strandat 4452.094 kb on - strandat 4452.096 kb on + strandat 4452.190 kb on - strand, within nuoIat 4452.422 kb on - strand, within nuoIat 4452.490 kb on + strand, within nuoIat 4452.621 kb on - strandat 4452.621 kb on - strandat 4452.640 kb on + strandat 4452.640 kb on + strandat 4452.641 kb on - strandat 4452.641 kb on - strandat 4452.693 kb on + strand, within nuoJat 4452.748 kb on - strand, within nuoJat 4452.995 kb on + strand, within nuoJat 4452.996 kb on - strand, within nuoJat 4453.015 kb on + strand, within nuoJat 4453.038 kb on - strand, within nuoJat 4453.164 kb on + strandat 4453.337 kb on + strand, within nuoKat 4453.338 kb on - strand, within nuoKat 4453.477 kb on - strandat 4453.583 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,451,203 + nuoH OKFHMN_22870 0.15 -2.7
4,451,264 - nuoH OKFHMN_22870 0.21 -0.4
4,451,467 + nuoH OKFHMN_22870 0.42 -0.5
4,451,467 + nuoH OKFHMN_22870 0.42 -2.4
4,451,467 + nuoH OKFHMN_22870 0.42 -3.0
4,451,780 + nuoH OKFHMN_22870 0.74 -1.1
4,452,093 + +1.6
4,452,094 - +0.7
4,452,096 + +1.1
4,452,190 - nuoI OKFHMN_22875 0.26 -1.7
4,452,422 - nuoI OKFHMN_22875 0.69 -2.7
4,452,490 + nuoI OKFHMN_22875 0.81 -0.3
4,452,621 - -1.5
4,452,621 - -0.3
4,452,640 + -1.2
4,452,640 + -0.2
4,452,641 - -0.3
4,452,641 - +0.8
4,452,693 + nuoJ OKFHMN_22880 0.16 -0.8
4,452,748 - nuoJ OKFHMN_22880 0.26 +0.2
4,452,995 + nuoJ OKFHMN_22880 0.71 -2.3
4,452,996 - nuoJ OKFHMN_22880 0.71 +1.2
4,453,015 + nuoJ OKFHMN_22880 0.74 -3.2
4,453,038 - nuoJ OKFHMN_22880 0.78 +0.1
4,453,164 + -0.3
4,453,337 + nuoK OKFHMN_22885 0.60 +0.0
4,453,338 - nuoK OKFHMN_22885 0.61 +0.8
4,453,477 - +1.3
4,453,583 + -0.5

Or see this region's nucleotide sequence