Strain Fitness in Escherichia coli ECRC100 around OKFHMN_19515

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntubiH and ubiI are separated by 23 nucleotidesubiI and gcvT are separated by 447 nucleotides OKFHMN_19510: ubiH - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 3,785,744 to 3,786,922 ubiH OKFHMN_19515: ubiI - FAD-dependent 2-octaprenylphenol hydroxylase, at 3,786,946 to 3,788,148 ubiI OKFHMN_19520: gcvT - glycine cleavage system aminomethyltransferase GcvT, at 3,788,596 to 3,789,690 gcvT Position (kb) 3786 3787 3788 3789Strain fitness (log2 ratio) -2 -1 0 1 2at 3786.865 kb on - strandat 3786.941 kb on - strandat 3786.962 kb on + strandat 3787.404 kb on + strand, within ubiIat 3787.404 kb on + strand, within ubiIat 3787.597 kb on - strand, within ubiIat 3787.914 kb on + strand, within ubiIat 3787.936 kb on + strand, within ubiIat 3787.992 kb on + strand, within ubiIat 3788.330 kb on - strandat 3788.547 kb on - strandat 3788.579 kb on + strandat 3788.617 kb on + strandat 3788.714 kb on + strand, within gcvTat 3788.748 kb on + strand, within gcvTat 3788.748 kb on + strand, within gcvTat 3788.748 kb on + strand, within gcvTat 3788.882 kb on + strand, within gcvTat 3788.899 kb on + strand, within gcvTat 3788.903 kb on - strand, within gcvTat 3788.934 kb on + strand, within gcvTat 3789.035 kb on + strand, within gcvTat 3789.036 kb on - strand, within gcvTat 3789.075 kb on + strand, within gcvT

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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3,786,865 - +0.2
3,786,941 - -0.9
3,786,962 + +0.1
3,787,404 + ubiI OKFHMN_19515 0.38 -2.3
3,787,404 + ubiI OKFHMN_19515 0.38 +0.1
3,787,597 - ubiI OKFHMN_19515 0.54 -0.5
3,787,914 + ubiI OKFHMN_19515 0.80 -1.0
3,787,936 + ubiI OKFHMN_19515 0.82 +2.1
3,787,992 + ubiI OKFHMN_19515 0.87 -0.5
3,788,330 - -0.0
3,788,547 - +0.8
3,788,579 + -0.2
3,788,617 + -0.0
3,788,714 + gcvT OKFHMN_19520 0.11 -0.2
3,788,748 + gcvT OKFHMN_19520 0.14 -0.2
3,788,748 + gcvT OKFHMN_19520 0.14 +0.5
3,788,748 + gcvT OKFHMN_19520 0.14 -0.4
3,788,882 + gcvT OKFHMN_19520 0.26 +0.7
3,788,899 + gcvT OKFHMN_19520 0.28 +1.7
3,788,903 - gcvT OKFHMN_19520 0.28 -0.6
3,788,934 + gcvT OKFHMN_19520 0.31 +0.2
3,789,035 + gcvT OKFHMN_19520 0.40 +0.6
3,789,036 - gcvT OKFHMN_19520 0.40 +0.3
3,789,075 + gcvT OKFHMN_19520 0.44 -0.1

Or see this region's nucleotide sequence