Strain Fitness in Escherichia coli ECRC100 around OKFHMN_03240

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmcrA and OKFHMN_03235 are separated by 44 nucleotidesOKFHMN_03235 and OKFHMN_03240 are separated by 222 nucleotidesOKFHMN_03240 and OKFHMN_03245 are separated by 356 nucleotidesOKFHMN_03245 and OKFHMN_03250 are separated by 23 nucleotides OKFHMN_03230: mcrA - HNH endonuclease, at 514,279 to 514,644 mcrA OKFHMN_03235: OKFHMN_03235 - hypothetical protein, at 514,689 to 514,778 _03235 OKFHMN_03240: OKFHMN_03240 - hypothetical protein, at 515,001 to 515,141 _03240 OKFHMN_03245: OKFHMN_03245 - hypothetical protein, at 515,498 to 515,722 _03245 OKFHMN_03250: OKFHMN_03250 - lysis protein, at 515,746 to 516,213 _03250 Position (kb) 515 516Strain fitness (log2 ratio) -1 0 1at 514.865 kb on + strandat 514.866 kb on - strandat 514.869 kb on + strandat 514.989 kb on - strandat 515.078 kb on - strand, within OKFHMN_03240at 515.093 kb on - strand, within OKFHMN_03240at 515.181 kb on - strandat 515.213 kb on - strandat 515.490 kb on - strandat 515.514 kb on - strandat 515.754 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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514,865 + -1.0
514,866 - +0.4
514,869 + -0.2
514,989 - +0.4
515,078 - OKFHMN_03240 0.55 -1.5
515,093 - OKFHMN_03240 0.65 -0.7
515,181 - -0.9
515,213 - -0.2
515,490 - +0.5
515,514 - +0.2
515,754 + +0.3

Or see this region's nucleotide sequence