Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14915 and SGL_RS14920 are separated by 125 nucleotides SGL_RS14920 and SGL_RS14925 are separated by 225 nucleotides SGL_RS14925 and SGL_RS14930 are separated by 34 nucleotides
SGL_RS14915: SGL_RS14915 - ABC transporter substrate-binding protein, at 2,799,650 to 2,801,014
_RS14915
SGL_RS14920: SGL_RS14920 - cation:proton antiporter, at 2,801,140 to 2,803,035
_RS14920
SGL_RS14925: SGL_RS14925 - tRNA-Cys, at 2,803,261 to 2,803,331
_RS14925
SGL_RS14930: SGL_RS14930 - type I methionyl aminopeptidase, at 2,803,366 to 2,804,208
_RS14930
Position (kb)
2801
2802
2803
2804 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2800.148 kb on - strand, within SGL_RS14915 at 2800.148 kb on - strand, within SGL_RS14915 at 2800.418 kb on + strand, within SGL_RS14915 at 2800.426 kb on - strand, within SGL_RS14915 at 2800.426 kb on - strand, within SGL_RS14915 at 2800.426 kb on - strand, within SGL_RS14915 at 2800.971 kb on + strand at 2800.971 kb on + strand at 2801.288 kb on + strand at 2802.110 kb on - strand, within SGL_RS14920 at 2802.135 kb on + strand, within SGL_RS14920 at 2802.188 kb on + strand, within SGL_RS14920 at 2802.527 kb on + strand, within SGL_RS14920 at 2802.582 kb on - strand, within SGL_RS14920 at 2802.780 kb on + strand, within SGL_RS14920 at 2802.904 kb on + strand at 2803.071 kb on + strand at 2803.141 kb on - strand at 2803.563 kb on + strand, within SGL_RS14930 at 2803.566 kb on - strand, within SGL_RS14930 at 2803.625 kb on - strand, within SGL_RS14930 at 2803.646 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 2,800,148 - SGL_RS14915 0.36 +0.2 2,800,148 - SGL_RS14915 0.36 +0.1 2,800,418 + SGL_RS14915 0.56 +0.8 2,800,426 - SGL_RS14915 0.57 -0.2 2,800,426 - SGL_RS14915 0.57 -0.6 2,800,426 - SGL_RS14915 0.57 -0.3 2,800,971 + +1.1 2,800,971 + -0.2 2,801,288 + -3.0 2,802,110 - SGL_RS14920 0.51 -4.2 2,802,135 + SGL_RS14920 0.52 -3.7 2,802,188 + SGL_RS14920 0.55 -3.1 2,802,527 + SGL_RS14920 0.73 -3.8 2,802,582 - SGL_RS14920 0.76 -3.6 2,802,780 + SGL_RS14920 0.86 -2.8 2,802,904 + -3.7 2,803,071 + -0.8 2,803,141 - -0.2 2,803,563 + SGL_RS14930 0.23 -0.8 2,803,566 - SGL_RS14930 0.24 -1.6 2,803,625 - SGL_RS14930 0.31 -0.7 2,803,646 + SGL_RS14930 0.33 -0.7 2,803,781 + SGL_RS14930 0.49 -0.2 2,803,781 + SGL_RS14930 0.49 -0.3 2,803,781 + SGL_RS14930 0.49 -0.7 2,803,781 + SGL_RS14930 0.49 -1.4
Or see this region's nucleotide sequence