Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14530 and SGL_RS14535 are separated by 118 nucleotides SGL_RS14535 and SGL_RS14540 are separated by 13 nucleotides SGL_RS14540 and SGL_RS14545 are separated by 336 nucleotides
SGL_RS14530: SGL_RS14530 - carbamoyl-phosphate synthase large subunit, at 2,713,371 to 2,716,616
_RS14530
SGL_RS14535: SGL_RS14535 - hypothetical protein, at 2,716,735 to 2,717,163
_RS14535
SGL_RS14540: SGL_RS14540 - bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR, at 2,717,177 to 2,717,713
_RS14540
SGL_RS14545: SGL_RS14545 - GTP cyclohydrolase I FolE, at 2,718,050 to 2,718,754
_RS14545
Position (kb)
2717
2718 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2716.663 kb on + strand at 2716.712 kb on - strand at 2716.759 kb on + strand at 2716.767 kb on - strand at 2716.767 kb on - strand at 2716.821 kb on + strand, within SGL_RS14535 at 2717.058 kb on - strand, within SGL_RS14535 at 2717.498 kb on - strand, within SGL_RS14540 at 2717.583 kb on - strand, within SGL_RS14540 at 2717.608 kb on - strand, within SGL_RS14540 at 2717.707 kb on - strand at 2717.878 kb on + strand at 2717.880 kb on - strand at 2717.915 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 2,716,663 + +0.3 2,716,712 - -0.7 2,716,759 + +0.0 2,716,767 - +0.6 2,716,767 - +0.2 2,716,821 + SGL_RS14535 0.20 +0.3 2,717,058 - SGL_RS14535 0.75 +0.1 2,717,498 - SGL_RS14540 0.60 -2.0 2,717,583 - SGL_RS14540 0.76 -4.1 2,717,608 - SGL_RS14540 0.80 -3.1 2,717,707 - -4.2 2,717,878 + +0.3 2,717,880 - -0.1 2,717,915 - +0.2
Or see this region's nucleotide sequence