Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04650 and SGL_RS04655 are separated by 82 nucleotides SGL_RS04655 and SGL_RS04660 are separated by 186 nucleotides SGL_RS04660 and SGL_RS04665 are separated by 343 nucleotides
SGL_RS04650: SGL_RS04650 - tetratricopeptide repeat protein, at 594,454 to 595,650
_RS04650
SGL_RS04655: SGL_RS04655 - phycobiliprotein lyase, at 595,733 to 596,311
_RS04655
SGL_RS04660: SGL_RS04660 - phycobilisome rod-core linker polypeptide, at 596,498 to 597,247
_RS04660
SGL_RS04665: SGL_RS04665 - hypothetical protein, at 597,591 to 598,487
_RS04665
Position (kb) 596
597
598 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 595.524 kb on + strand, within SGL_RS04650 at 595.524 kb on + strand, within SGL_RS04650 at 595.594 kb on + strand at 595.664 kb on + strand at 596.489 kb on + strand at 596.509 kb on - strand at 596.570 kb on + strand at 596.755 kb on + strand, within SGL_RS04660 at 597.002 kb on - strand, within SGL_RS04660 at 597.168 kb on + strand, within SGL_RS04660 at 597.263 kb on - strand at 597.358 kb on - strand at 597.558 kb on - strand at 597.587 kb on + strand at 597.637 kb on + strand at 597.637 kb on + strand at 597.939 kb on - strand, within SGL_RS04665 at 598.040 kb on + strand, within SGL_RS04665 at 598.087 kb on + strand, within SGL_RS04665 at 598.087 kb on + strand, within SGL_RS04665
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 595,524 + SGL_RS04650 0.89 -0.3 595,524 + SGL_RS04650 0.89 +0.3 595,594 + -0.3 595,664 + +0.2 596,489 + -3.5 596,509 - -3.3 596,570 + -1.7 596,755 + SGL_RS04660 0.34 -3.0 597,002 - SGL_RS04660 0.67 -4.5 597,168 + SGL_RS04660 0.89 -1.9 597,263 - -0.8 597,358 - +0.4 597,558 - -0.3 597,587 + +0.0 597,637 + -0.1 597,637 + -0.1 597,939 - SGL_RS04665 0.39 -0.1 598,040 + SGL_RS04665 0.50 -0.3 598,087 + SGL_RS04665 0.55 +0.2 598,087 + SGL_RS04665 0.55 -0.0
Or see this region's nucleotide sequence