Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04515 and SGL_RS04520 are separated by 235 nucleotides SGL_RS04520 and SGL_RS04525 are separated by 55 nucleotides SGL_RS04525 and SGL_RS04530 are separated by 85 nucleotides SGL_RS04530 and SGL_RS04535 are separated by 36 nucleotides SGL_RS04535 and SGL_RS04540 are separated by 9 nucleotides SGL_RS04540 and SGL_RS04545 are separated by 32 nucleotides SGL_RS04545 and SGL_RS04550 are separated by 84 nucleotides
SGL_RS04515: SGL_RS04515 - 1-deoxy-D-xylulose-5-phosphate synthase, at 566,983 to 568,905
_RS04515
SGL_RS04520: SGL_RS04520 - rubredoxin, at 569,141 to 569,488
_RS04520
SGL_RS04525: SGL_RS04525 - photosynthesis system II assembly factor Ycf48, at 569,544 to 570,572
_RS04525
SGL_RS04530: SGL_RS04530 - cytochrome b559 subunit alpha, at 570,658 to 570,903
_RS04530
SGL_RS04535: SGL_RS04535 - cytochrome b559 subunit beta, at 570,940 to 571,074
_RS04535
SGL_RS04540: SGL_RS04540 - photosystem II reaction center protein L, at 571,084 to 571,203
_RS04540
SGL_RS04545: SGL_RS04545 - photosystem II reaction center protein J, at 571,236 to 571,355
_RS04545
SGL_RS04550: SGL_RS04550 - glutamate 5-kinase, at 571,440 to 572,567
_RS04550
Position (kb) 569
570
571 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 569.046 kb on - strand at 569.334 kb on + strand, within SGL_RS04520 at 569.483 kb on + strand at 569.484 kb on + strand at 569.651 kb on + strand, within SGL_RS04525 at 569.693 kb on - strand, within SGL_RS04525 at 569.751 kb on - strand, within SGL_RS04525 at 569.751 kb on - strand, within SGL_RS04525 at 569.806 kb on + strand, within SGL_RS04525 at 569.950 kb on + strand, within SGL_RS04525 at 569.976 kb on + strand, within SGL_RS04525 at 570.277 kb on - strand, within SGL_RS04525 at 570.409 kb on + strand, within SGL_RS04525 at 571.281 kb on - strand, within SGL_RS04545 at 571.520 kb on - strand at 571.570 kb on + strand, within SGL_RS04550
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 569,046 - +0.4 569,334 + SGL_RS04520 0.55 -2.1 569,483 + -0.4 569,484 + -0.5 569,651 + SGL_RS04525 0.10 -3.3 569,693 - SGL_RS04525 0.14 -1.8 569,751 - SGL_RS04525 0.20 -2.7 569,751 - SGL_RS04525 0.20 -3.4 569,806 + SGL_RS04525 0.25 -3.9 569,950 + SGL_RS04525 0.39 -2.9 569,976 + SGL_RS04525 0.42 -0.7 570,277 - SGL_RS04525 0.71 +0.9 570,409 + SGL_RS04525 0.84 -1.4 571,281 - SGL_RS04545 0.38 -1.3 571,520 - -1.1 571,570 + SGL_RS04550 0.12 +0.1
Or see this region's nucleotide sequence