Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00810 and SGL_RS00815 overlap by 10 nucleotides SGL_RS00815 and SGL_RS00820 are separated by 65 nucleotides SGL_RS00820 and SGL_RS00825 are separated by 32 nucleotides SGL_RS00825 and SGL_RS00830 overlap by 8 nucleotides
SGL_RS00810: SGL_RS00810 - AbrB/MazE/SpoVT family DNA-binding domain-containing protein, at 36,597 to 36,848
_RS00810
SGL_RS00815: SGL_RS00815 - type II toxin-antitoxin system PemK/MazF family toxin, at 36,839 to 37,201
_RS00815
SGL_RS00820: SGL_RS00820 - DUF1819 family protein, at 37,267 to 37,521
_RS00820
SGL_RS00825: SGL_RS00825 - hypothetical protein, at 37,554 to 38,138
_RS00825
SGL_RS00830: SGL_RS00830 - hypothetical protein, at 38,131 to 38,712
_RS00830
Position (kb) 37
38
39 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 36.582 kb on + strand at 36.653 kb on - strand, within SGL_RS00810 at 36.658 kb on - strand, within SGL_RS00810 at 36.801 kb on - strand, within SGL_RS00810 at 36.855 kb on - strand at 36.857 kb on - strand at 36.880 kb on - strand, within SGL_RS00815 at 37.136 kb on + strand, within SGL_RS00815 at 37.171 kb on - strand at 37.202 kb on + strand at 37.229 kb on - strand at 37.241 kb on - strand at 37.331 kb on - strand, within SGL_RS00820 at 37.344 kb on - strand, within SGL_RS00820 at 37.454 kb on + strand, within SGL_RS00820 at 37.472 kb on - strand, within SGL_RS00820 at 37.476 kb on + strand, within SGL_RS00820 at 37.476 kb on + strand, within SGL_RS00820 at 37.581 kb on + strand at 37.690 kb on + strand, within SGL_RS00825 at 37.722 kb on - strand, within SGL_RS00825 at 37.748 kb on + strand, within SGL_RS00825 at 37.751 kb on - strand, within SGL_RS00825 at 37.756 kb on - strand, within SGL_RS00825 at 37.785 kb on - strand, within SGL_RS00825 at 37.785 kb on - strand, within SGL_RS00825 at 37.925 kb on - strand, within SGL_RS00825 at 38.020 kb on - strand, within SGL_RS00825 at 38.218 kb on + strand, within SGL_RS00830 at 38.326 kb on + strand, within SGL_RS00830 at 38.341 kb on + strand, within SGL_RS00830 at 38.516 kb on + strand, within SGL_RS00830 at 38.581 kb on + strand, within SGL_RS00830 at 38.673 kb on + strand at 38.679 kb on - strand at 38.703 kb on - strand at 38.739 kb on + strand at 38.746 kb on + strand at 38.771 kb on + strand at 38.825 kb on + strand at 38.926 kb on - strand at 38.948 kb on + strand at 39.086 kb on + strand at 39.090 kb on + strand at 39.100 kb on + strand at 39.108 kb on - strand at 39.137 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 36,582 + -0.4 36,653 - SGL_RS00810 0.22 +0.7 36,658 - SGL_RS00810 0.24 -0.4 36,801 - SGL_RS00810 0.81 +0.1 36,855 - -0.2 36,857 - +0.4 36,880 - SGL_RS00815 0.11 +0.2 37,136 + SGL_RS00815 0.82 -0.2 37,171 - -0.2 37,202 + +0.1 37,229 - -0.3 37,241 - +0.3 37,331 - SGL_RS00820 0.25 -0.2 37,344 - SGL_RS00820 0.30 -0.0 37,454 + SGL_RS00820 0.73 +0.0 37,472 - SGL_RS00820 0.80 -0.0 37,476 + SGL_RS00820 0.82 +0.0 37,476 + SGL_RS00820 0.82 +0.1 37,581 + -2.1 37,690 + SGL_RS00825 0.23 -2.4 37,722 - SGL_RS00825 0.29 -1.8 37,748 + SGL_RS00825 0.33 -2.5 37,751 - SGL_RS00825 0.34 -1.9 37,756 - SGL_RS00825 0.35 -2.3 37,785 - SGL_RS00825 0.39 -0.7 37,785 - SGL_RS00825 0.39 -1.7 37,925 - SGL_RS00825 0.63 -2.3 38,020 - SGL_RS00825 0.80 -1.3 38,218 + SGL_RS00830 0.15 -3.8 38,326 + SGL_RS00830 0.34 -1.4 38,341 + SGL_RS00830 0.36 -0.3 38,516 + SGL_RS00830 0.66 -1.3 38,581 + SGL_RS00830 0.77 -2.2 38,673 + -1.5 38,679 - -2.1 38,703 - -1.8 38,739 + +0.1 38,746 + +0.4 38,771 + +0.2 38,825 + +0.1 38,926 - -0.0 38,948 + -0.1 39,086 + +0.3 39,090 + +0.3 39,100 + +0.5 39,108 - -0.7 39,137 + -0.1
Or see this region's nucleotide sequence