Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08820 and SGL_RS08825 are separated by 137 nucleotides SGL_RS08825 and SGL_RS08830 are separated by 167 nucleotides SGL_RS08830 and SGL_RS08835 are separated by 20 nucleotides
SGL_RS08820: SGL_RS08820 - metal ABC transporter substrate-binding protein, at 1,482,261 to 1,483,253
_RS08820
SGL_RS08825: SGL_RS08825 - IS5 family transposase, at 1,483,391 to 1,484,044
_RS08825
SGL_RS08830: SGL_RS08830 - circadian clock KaiB family protein, at 1,484,212 to 1,484,538
_RS08830
SGL_RS08835: SGL_RS08835 - circadian clock protein KaiC, at 1,484,559 to 1,486,265
_RS08835
Position (kb) 1484
1485 Strain fitness (log2 ratio) -1
0
1 at 1483.255 kb on + strand at 1483.257 kb on - strand at 1483.276 kb on - strand at 1483.366 kb on + strand at 1483.421 kb on - strand at 1483.422 kb on + strand at 1483.702 kb on - strand, within SGL_RS08825 at 1483.840 kb on - strand, within SGL_RS08825 at 1484.597 kb on + strand at 1484.755 kb on - strand, within SGL_RS08835 at 1484.762 kb on - strand, within SGL_RS08835
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 1,483,255 + -0.3 1,483,257 - -0.3 1,483,276 - -0.1 1,483,366 + +0.1 1,483,421 - -0.2 1,483,422 + -0.1 1,483,702 - SGL_RS08825 0.48 -0.5 1,483,840 - SGL_RS08825 0.69 +0.0 1,484,597 + -1.7 1,484,755 - SGL_RS08835 0.11 -0.1 1,484,762 - SGL_RS08835 0.12 +0.0
Or see this region's nucleotide sequence