Experiment: HMW_DNA_from_Pseudomonas_EB271-B4-1 46.9 ppm (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS04340 and A4249_RS04345 are separated by 2 nucleotides A4249_RS04345 and A4249_RS04350 overlap by 4 nucleotides A4249_RS04350 and A4249_RS04355 are separated by 11 nucleotides
A4249_RS04340: A4249_RS04340 - glycosyltransferase family 2 protein, at 898,634 to 899,566
_RS04340
A4249_RS04345: A4249_RS04345 - glycosyltransferase family A protein, at 899,569 to 900,501
_RS04345
A4249_RS04350: A4249_RS04350 - lipopolysaccharide biosynthesis protein, at 900,498 to 901,943
_RS04350
A4249_RS04355: A4249_RS04355 - exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase, at 901,955 to 903,439
_RS04355
Position (kb)
900
901
902 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 899.665 kb on - strand, within A4249_RS04345 at 899.720 kb on + strand, within A4249_RS04345 at 899.720 kb on + strand, within A4249_RS04345 at 899.720 kb on + strand, within A4249_RS04345 at 899.721 kb on - strand, within A4249_RS04345 at 899.721 kb on - strand, within A4249_RS04345 at 899.729 kb on + strand, within A4249_RS04345 at 899.729 kb on + strand, within A4249_RS04345 at 899.729 kb on + strand, within A4249_RS04345 at 899.830 kb on - strand, within A4249_RS04345 at 899.976 kb on + strand, within A4249_RS04345 at 899.976 kb on + strand, within A4249_RS04345 at 899.976 kb on + strand, within A4249_RS04345 at 899.978 kb on + strand, within A4249_RS04345 at 899.978 kb on + strand, within A4249_RS04345 at 899.979 kb on - strand, within A4249_RS04345 at 899.979 kb on - strand, within A4249_RS04345 at 899.984 kb on + strand, within A4249_RS04345 at 899.985 kb on - strand, within A4249_RS04345 at 900.194 kb on + strand, within A4249_RS04345 at 900.195 kb on - strand, within A4249_RS04345 at 900.195 kb on - strand, within A4249_RS04345 at 900.195 kb on - strand, within A4249_RS04345 at 900.292 kb on - strand, within A4249_RS04345 at 900.491 kb on + strand at 900.492 kb on - strand at 900.492 kb on - strand at 900.492 kb on - strand at 900.586 kb on + strand at 900.586 kb on + strand at 900.587 kb on - strand at 900.587 kb on - strand at 900.916 kb on + strand, within A4249_RS04350 at 900.916 kb on + strand, within A4249_RS04350 at 900.917 kb on - strand, within A4249_RS04350 at 900.919 kb on + strand, within A4249_RS04350 at 901.204 kb on + strand, within A4249_RS04350 at 901.205 kb on - strand, within A4249_RS04350 at 901.205 kb on - strand, within A4249_RS04350 at 901.205 kb on - strand, within A4249_RS04350 at 901.318 kb on + strand, within A4249_RS04350 at 901.327 kb on + strand, within A4249_RS04350 at 901.327 kb on + strand, within A4249_RS04350 at 901.328 kb on - strand, within A4249_RS04350 at 901.479 kb on + strand, within A4249_RS04350 at 901.700 kb on - strand, within A4249_RS04350 at 901.918 kb on + strand at 901.919 kb on - strand at 901.919 kb on - strand at 901.919 kb on - strand at 901.919 kb on - strand at 901.993 kb on + strand at 901.994 kb on - strand at 901.994 kb on - strand at 902.041 kb on + strand at 902.041 kb on + strand at 902.043 kb on + strand at 902.043 kb on + strand at 902.043 kb on + strand at 902.043 kb on + strand at 902.043 kb on + strand at 902.044 kb on - strand at 902.044 kb on - strand at 902.044 kb on - strand at 902.044 kb on - strand at 902.044 kb on - strand at 902.044 kb on - strand at 902.044 kb on - strand at 902.160 kb on + strand, within A4249_RS04355 at 902.160 kb on + strand, within A4249_RS04355 at 902.161 kb on - strand, within A4249_RS04355 at 902.401 kb on + strand, within A4249_RS04355 at 902.401 kb on + strand, within A4249_RS04355 at 902.401 kb on + strand, within A4249_RS04355 at 902.401 kb on + strand, within A4249_RS04355 at 902.401 kb on + strand, within A4249_RS04355 at 902.401 kb on + strand, within A4249_RS04355 at 902.401 kb on + strand, within A4249_RS04355 at 902.401 kb on + strand, within A4249_RS04355 at 902.401 kb on + strand, within A4249_RS04355 at 902.401 kb on + strand, within A4249_RS04355 at 902.402 kb on - strand, within A4249_RS04355 at 902.402 kb on - strand, within A4249_RS04355 at 902.402 kb on - strand, within A4249_RS04355 at 902.402 kb on - strand, within A4249_RS04355 at 902.402 kb on - strand, within A4249_RS04355 at 902.402 kb on - strand, within A4249_RS04355 at 902.402 kb on - strand, within A4249_RS04355 at 902.402 kb on - strand, within A4249_RS04355 at 902.402 kb on - strand, within A4249_RS04355 at 902.526 kb on - strand, within A4249_RS04355 at 902.526 kb on - strand, within A4249_RS04355 at 902.723 kb on + strand, within A4249_RS04355 at 902.723 kb on + strand, within A4249_RS04355 at 902.723 kb on + strand, within A4249_RS04355 at 902.723 kb on + strand, within A4249_RS04355 at 902.723 kb on + strand, within A4249_RS04355 at 902.723 kb on + strand, within A4249_RS04355 at 902.723 kb on + strand, within A4249_RS04355 at 902.724 kb on - strand, within A4249_RS04355 at 902.724 kb on - strand, within A4249_RS04355 at 902.724 kb on - strand, within A4249_RS04355 at 902.748 kb on + strand, within A4249_RS04355 at 902.748 kb on + strand, within A4249_RS04355 at 902.748 kb on + strand, within A4249_RS04355 at 902.749 kb on - strand, within A4249_RS04355 at 902.749 kb on - strand, within A4249_RS04355 at 902.749 kb on - strand, within A4249_RS04355 at 902.749 kb on - strand, within A4249_RS04355
Per-strain Table
Position Strand Gene LocusTag Fraction HMW_DNA_from_Pseudomonas_EB271-B4-1 46.9 ppm (C) remove 899,665 - A4249_RS04345 0.10 -1.1 899,720 + A4249_RS04345 0.16 +1.1 899,720 + A4249_RS04345 0.16 +1.0 899,720 + A4249_RS04345 0.16 -1.0 899,721 - A4249_RS04345 0.16 +0.1 899,721 - A4249_RS04345 0.16 -0.5 899,729 + A4249_RS04345 0.17 +0.3 899,729 + A4249_RS04345 0.17 +0.4 899,729 + A4249_RS04345 0.17 +0.2 899,830 - A4249_RS04345 0.28 +1.1 899,976 + A4249_RS04345 0.44 +1.3 899,976 + A4249_RS04345 0.44 -0.5 899,976 + A4249_RS04345 0.44 +0.1 899,978 + A4249_RS04345 0.44 +0.5 899,978 + A4249_RS04345 0.44 -0.1 899,979 - A4249_RS04345 0.44 -0.0 899,979 - A4249_RS04345 0.44 -0.3 899,984 + A4249_RS04345 0.44 +1.2 899,985 - A4249_RS04345 0.45 -0.8 900,194 + A4249_RS04345 0.67 -0.9 900,195 - A4249_RS04345 0.67 -1.0 900,195 - A4249_RS04345 0.67 -0.3 900,195 - A4249_RS04345 0.67 +1.1 900,292 - A4249_RS04345 0.77 +0.2 900,491 + +0.7 900,492 - -0.3 900,492 - +0.1 900,492 - -0.2 900,586 + +0.9 900,586 + +0.1 900,587 - +2.2 900,587 - +0.8 900,916 + A4249_RS04350 0.29 +2.7 900,916 + A4249_RS04350 0.29 -0.3 900,917 - A4249_RS04350 0.29 -0.5 900,919 + A4249_RS04350 0.29 +0.7 901,204 + A4249_RS04350 0.49 -0.6 901,205 - A4249_RS04350 0.49 +0.4 901,205 - A4249_RS04350 0.49 -0.5 901,205 - A4249_RS04350 0.49 +0.2 901,318 + A4249_RS04350 0.57 +1.5 901,327 + A4249_RS04350 0.57 +0.1 901,327 + A4249_RS04350 0.57 -0.8 901,328 - A4249_RS04350 0.57 -0.5 901,479 + A4249_RS04350 0.68 -0.1 901,700 - A4249_RS04350 0.83 -0.4 901,918 + -1.8 901,919 - +1.9 901,919 - -0.6 901,919 - +2.1 901,919 - +1.8 901,993 + +0.5 901,994 - +0.1 901,994 - +0.0 902,041 + -0.2 902,041 + -0.3 902,043 + -0.2 902,043 + -0.4 902,043 + -0.2 902,043 + -0.6 902,043 + -0.2 902,044 - +0.8 902,044 - -0.0 902,044 - -1.6 902,044 - -0.1 902,044 - +0.4 902,044 - -0.2 902,044 - +1.0 902,160 + A4249_RS04355 0.14 +1.2 902,160 + A4249_RS04355 0.14 -0.6 902,161 - A4249_RS04355 0.14 +0.5 902,401 + A4249_RS04355 0.30 +0.1 902,401 + A4249_RS04355 0.30 +1.2 902,401 + A4249_RS04355 0.30 -0.8 902,401 + A4249_RS04355 0.30 +0.6 902,401 + A4249_RS04355 0.30 -0.8 902,401 + A4249_RS04355 0.30 +0.5 902,401 + A4249_RS04355 0.30 -0.1 902,401 + A4249_RS04355 0.30 +0.7 902,401 + A4249_RS04355 0.30 +1.3 902,401 + A4249_RS04355 0.30 +0.5 902,402 - A4249_RS04355 0.30 -0.9 902,402 - A4249_RS04355 0.30 -0.5 902,402 - A4249_RS04355 0.30 -0.3 902,402 - A4249_RS04355 0.30 +0.5 902,402 - A4249_RS04355 0.30 +0.2 902,402 - A4249_RS04355 0.30 +0.3 902,402 - A4249_RS04355 0.30 -0.5 902,402 - A4249_RS04355 0.30 +0.5 902,402 - A4249_RS04355 0.30 +0.0 902,526 - A4249_RS04355 0.38 -0.6 902,526 - A4249_RS04355 0.38 -0.9 902,723 + A4249_RS04355 0.52 -0.5 902,723 + A4249_RS04355 0.52 -0.6 902,723 + A4249_RS04355 0.52 +0.3 902,723 + A4249_RS04355 0.52 -0.0 902,723 + A4249_RS04355 0.52 +0.8 902,723 + A4249_RS04355 0.52 +0.6 902,723 + A4249_RS04355 0.52 +0.5 902,724 - A4249_RS04355 0.52 -1.5 902,724 - A4249_RS04355 0.52 -0.4 902,724 - A4249_RS04355 0.52 -0.4 902,748 + A4249_RS04355 0.53 -1.8 902,748 + A4249_RS04355 0.53 -0.4 902,748 + A4249_RS04355 0.53 -0.2 902,749 - A4249_RS04355 0.53 -1.1 902,749 - A4249_RS04355 0.53 -0.6 902,749 - A4249_RS04355 0.53 -0.4 902,749 - A4249_RS04355 0.53 -0.3
Or see this region's nucleotide sequence