Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2026 and DORF15085 are separated by 20 nucleotides DORF15085 and DVU2028 are separated by 82 nucleotides DVU2028 and DVU2029 are separated by 44 nucleotides DVU2029 and DVU2030 overlap by 4 nucleotides
DVU2026: DVU2026 - conserved hypothetical protein (TIGR), at 2,109,863 to 2,110,453
DVU2026
DORF15085: DORF15085 - no description, at 2,110,474 to 2,111,085
DORF15085
DVU2028: DVU2028 - conserved domain protein (TIGR), at 2,111,168 to 2,111,395
DVU2028
DVU2029: DVU2029 - ISDvu2, transposase OrfA (TIGR), at 2,111,440 to 2,111,838
DVU2029
DVU2030: DVU2030 - ISDvu2, transposase OrfB (TIGR), at 2,111,835 to 2,112,677
DVU2030
Position (kb)
2111
2112 Strain fitness (log2 ratio)
-1
0
1 at 2110.169 kb on - strand, within DVU2026 at 2110.179 kb on - strand, within DVU2026 at 2110.293 kb on - strand, within DVU2026 at 2110.417 kb on + strand at 2110.429 kb on + strand at 2111.229 kb on + strand, within DVU2028 at 2111.244 kb on + strand, within DVU2028 at 2111.258 kb on + strand, within DVU2028 at 2111.266 kb on + strand, within DVU2028 at 2111.302 kb on + strand, within DVU2028 at 2111.302 kb on + strand, within DVU2028 at 2111.310 kb on - strand, within DVU2028 at 2111.311 kb on - strand, within DVU2028 at 2111.334 kb on + strand, within DVU2028 at 2111.334 kb on + strand, within DVU2028 at 2111.342 kb on - strand, within DVU2028
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 2,110,169 - DVU2026 0.52 -1.7 2,110,179 - DVU2026 0.53 -1.4 2,110,293 - DVU2026 0.73 +1.1 2,110,417 + +0.8 2,110,429 + +0.3 2,111,229 + DVU2028 0.27 +1.1 2,111,244 + DVU2028 0.33 +0.4 2,111,258 + DVU2028 0.39 +0.1 2,111,266 + DVU2028 0.43 +0.2 2,111,302 + DVU2028 0.59 +0.3 2,111,302 + DVU2028 0.59 -0.6 2,111,310 - DVU2028 0.62 +0.2 2,111,311 - DVU2028 0.63 -0.3 2,111,334 + DVU2028 0.73 +0.5 2,111,334 + DVU2028 0.73 -0.1 2,111,342 - DVU2028 0.76 -0.2
Or see this region's nucleotide sequence