Experiment: Thymine 0.793 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS09275 and A4249_RS09280 are separated by 74 nucleotides A4249_RS09280 and A4249_RS09285 are separated by 108 nucleotides A4249_RS09285 and A4249_RS09290 overlap by 1 nucleotides
A4249_RS09275: A4249_RS09275 - DUF3035 domain-containing protein, at 1,856,438 to 1,857,004
_RS09275
A4249_RS09280: A4249_RS09280 - PaaI family thioesterase, at 1,857,079 to 1,857,522
_RS09280
A4249_RS09285: A4249_RS09285 - Tat pathway signal sequence domain protein, at 1,857,631 to 1,858,317
_RS09285
A4249_RS09290: A4249_RS09290 - arginine--tRNA ligase, at 1,858,317 to 1,860,119
_RS09290
Position (kb)
1857
1858
1859 Strain fitness (log2 ratio)
-1
0
1 at 1856.762 kb on + strand, within A4249_RS09275 at 1856.762 kb on + strand, within A4249_RS09275 at 1856.763 kb on - strand, within A4249_RS09275 at 1856.763 kb on - strand, within A4249_RS09275 at 1856.763 kb on - strand, within A4249_RS09275 at 1856.763 kb on - strand, within A4249_RS09275 at 1856.763 kb on - strand, within A4249_RS09275 at 1857.002 kb on + strand at 1857.003 kb on - strand at 1857.047 kb on - strand at 1857.047 kb on - strand at 1857.047 kb on - strand at 1857.123 kb on - strand at 1857.123 kb on - strand at 1857.123 kb on - strand at 1857.123 kb on - strand at 1857.123 kb on - strand at 1857.241 kb on + strand, within A4249_RS09280 at 1857.241 kb on + strand, within A4249_RS09280 at 1857.241 kb on + strand, within A4249_RS09280 at 1857.242 kb on - strand, within A4249_RS09280 at 1857.242 kb on - strand, within A4249_RS09280 at 1857.242 kb on - strand, within A4249_RS09280 at 1857.242 kb on - strand, within A4249_RS09280 at 1857.242 kb on - strand, within A4249_RS09280 at 1857.243 kb on + strand, within A4249_RS09280 at 1857.243 kb on + strand, within A4249_RS09280 at 1857.243 kb on + strand, within A4249_RS09280 at 1857.243 kb on + strand, within A4249_RS09280 at 1857.244 kb on - strand, within A4249_RS09280 at 1857.244 kb on - strand, within A4249_RS09280 at 1857.244 kb on - strand, within A4249_RS09280 at 1857.244 kb on - strand, within A4249_RS09280 at 1857.244 kb on - strand, within A4249_RS09280 at 1857.340 kb on + strand, within A4249_RS09280 at 1857.340 kb on + strand, within A4249_RS09280 at 1857.340 kb on + strand, within A4249_RS09280 at 1857.341 kb on - strand, within A4249_RS09280 at 1857.341 kb on - strand, within A4249_RS09280 at 1857.341 kb on - strand, within A4249_RS09280 at 1857.341 kb on - strand, within A4249_RS09280 at 1857.342 kb on + strand, within A4249_RS09280 at 1857.343 kb on - strand, within A4249_RS09280 at 1857.343 kb on - strand, within A4249_RS09280 at 1857.343 kb on - strand, within A4249_RS09280 at 1857.481 kb on + strand at 1857.481 kb on + strand at 1857.481 kb on + strand at 1857.482 kb on - strand at 1857.552 kb on + strand at 1857.553 kb on - strand at 1857.559 kb on + strand at 1857.829 kb on + strand, within A4249_RS09285 at 1857.829 kb on + strand, within A4249_RS09285 at 1857.844 kb on + strand, within A4249_RS09285 at 1857.844 kb on + strand, within A4249_RS09285 at 1857.859 kb on + strand, within A4249_RS09285 at 1857.859 kb on + strand, within A4249_RS09285 at 1858.030 kb on + strand, within A4249_RS09285 at 1858.030 kb on + strand, within A4249_RS09285 at 1858.030 kb on + strand, within A4249_RS09285 at 1858.030 kb on + strand, within A4249_RS09285 at 1858.030 kb on + strand, within A4249_RS09285 at 1858.084 kb on + strand, within A4249_RS09285 at 1858.084 kb on + strand, within A4249_RS09285 at 1858.084 kb on + strand, within A4249_RS09285 at 1858.315 kb on + strand at 1858.315 kb on + strand at 1858.315 kb on + strand at 1858.315 kb on + strand at 1858.315 kb on + strand at 1858.316 kb on - strand at 1858.316 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine 0.793 mM (C) remove 1,856,762 + A4249_RS09275 0.57 +0.1 1,856,762 + A4249_RS09275 0.57 -1.1 1,856,763 - A4249_RS09275 0.57 -0.5 1,856,763 - A4249_RS09275 0.57 -0.6 1,856,763 - A4249_RS09275 0.57 +0.3 1,856,763 - A4249_RS09275 0.57 -1.0 1,856,763 - A4249_RS09275 0.57 -0.9 1,857,002 + +0.3 1,857,003 - +0.2 1,857,047 - -0.8 1,857,047 - +0.2 1,857,047 - +1.0 1,857,123 - -0.0 1,857,123 - +0.4 1,857,123 - +0.2 1,857,123 - -0.3 1,857,123 - +0.5 1,857,241 + A4249_RS09280 0.36 -0.5 1,857,241 + A4249_RS09280 0.36 -1.1 1,857,241 + A4249_RS09280 0.36 +0.1 1,857,242 - A4249_RS09280 0.37 -0.2 1,857,242 - A4249_RS09280 0.37 -0.1 1,857,242 - A4249_RS09280 0.37 -0.5 1,857,242 - A4249_RS09280 0.37 +0.3 1,857,242 - A4249_RS09280 0.37 -0.8 1,857,243 + A4249_RS09280 0.37 +0.6 1,857,243 + A4249_RS09280 0.37 +0.7 1,857,243 + A4249_RS09280 0.37 -0.0 1,857,243 + A4249_RS09280 0.37 +0.2 1,857,244 - A4249_RS09280 0.37 -0.2 1,857,244 - A4249_RS09280 0.37 +0.2 1,857,244 - A4249_RS09280 0.37 -0.7 1,857,244 - A4249_RS09280 0.37 +0.3 1,857,244 - A4249_RS09280 0.37 +0.5 1,857,340 + A4249_RS09280 0.59 +1.4 1,857,340 + A4249_RS09280 0.59 -0.6 1,857,340 + A4249_RS09280 0.59 +0.2 1,857,341 - A4249_RS09280 0.59 -0.1 1,857,341 - A4249_RS09280 0.59 -0.3 1,857,341 - A4249_RS09280 0.59 -1.0 1,857,341 - A4249_RS09280 0.59 +0.2 1,857,342 + A4249_RS09280 0.59 +0.4 1,857,343 - A4249_RS09280 0.59 -0.4 1,857,343 - A4249_RS09280 0.59 -0.5 1,857,343 - A4249_RS09280 0.59 +0.5 1,857,481 + -0.4 1,857,481 + -0.3 1,857,481 + +0.3 1,857,482 - -0.1 1,857,552 + +0.8 1,857,553 - +0.3 1,857,559 + -0.2 1,857,829 + A4249_RS09285 0.29 -0.6 1,857,829 + A4249_RS09285 0.29 -1.3 1,857,844 + A4249_RS09285 0.31 +0.4 1,857,844 + A4249_RS09285 0.31 -1.6 1,857,859 + A4249_RS09285 0.33 +0.2 1,857,859 + A4249_RS09285 0.33 +0.4 1,858,030 + A4249_RS09285 0.58 -0.8 1,858,030 + A4249_RS09285 0.58 -0.7 1,858,030 + A4249_RS09285 0.58 +0.2 1,858,030 + A4249_RS09285 0.58 -0.2 1,858,030 + A4249_RS09285 0.58 +0.6 1,858,084 + A4249_RS09285 0.66 -0.0 1,858,084 + A4249_RS09285 0.66 -0.5 1,858,084 + A4249_RS09285 0.66 -1.6 1,858,315 + +0.2 1,858,315 + -0.3 1,858,315 + -0.8 1,858,315 + -0.7 1,858,315 + -0.1 1,858,316 - +0.3 1,858,316 - +0.1
Or see this region's nucleotide sequence