Experiment: Parafilmed volatile R2A_HEPES agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS06975 and ABIE51_RS06980 overlap by 4 nucleotides ABIE51_RS06980 and ABIE51_RS06985 are separated by 0 nucleotides ABIE51_RS06985 and ABIE51_RS06990 are separated by 4 nucleotides
ABIE51_RS06975: ABIE51_RS06975 - type I methionyl aminopeptidase, at 1,504,529 to 1,505,305
_RS06975
ABIE51_RS06980: ABIE51_RS06980 - [protein-PII] uridylyltransferase, at 1,505,302 to 1,507,936
_RS06980
ABIE51_RS06985: ABIE51_RS06985 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 1,507,937 to 1,508,836
_RS06985
ABIE51_RS06990: ABIE51_RS06990 - Spx/MgsR family RNA polymerase-binding regulatory protein, at 1,508,841 to 1,509,206
_RS06990
Position (kb)
1505
1506
1507
1508 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1504.361 kb on - strand at 1504.361 kb on - strand at 1504.361 kb on - strand at 1504.361 kb on - strand at 1504.451 kb on + strand at 1504.468 kb on + strand at 1504.469 kb on - strand at 1504.519 kb on + strand at 1504.520 kb on - strand at 1504.520 kb on - strand at 1505.301 kb on + strand at 1505.301 kb on + strand at 1505.301 kb on + strand at 1505.302 kb on - strand at 1505.302 kb on - strand at 1505.390 kb on + strand at 1505.390 kb on + strand at 1506.645 kb on - strand, within ABIE51_RS06980 at 1506.645 kb on - strand, within ABIE51_RS06980 at 1508.853 kb on + strand at 1508.919 kb on + strand, within ABIE51_RS06990 at 1508.920 kb on - strand, within ABIE51_RS06990 at 1508.934 kb on + strand, within ABIE51_RS06990
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile R2A_HEPES agar plate with no fungus remove 1,504,361 - +0.8 1,504,361 - +0.2 1,504,361 - -0.3 1,504,361 - +0.6 1,504,451 + +0.7 1,504,468 + -0.3 1,504,469 - -0.5 1,504,519 + +0.2 1,504,520 - +0.5 1,504,520 - +1.8 1,505,301 + +0.1 1,505,301 + +0.1 1,505,301 + +0.2 1,505,302 - -0.1 1,505,302 - +0.3 1,505,390 + -0.5 1,505,390 + +0.3 1,506,645 - ABIE51_RS06980 0.51 -2.4 1,506,645 - ABIE51_RS06980 0.51 +0.7 1,508,853 + +0.4 1,508,919 + ABIE51_RS06990 0.21 +0.9 1,508,920 - ABIE51_RS06990 0.22 -0.0 1,508,934 + ABIE51_RS06990 0.25 -0.5
Or see this region's nucleotide sequence