Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptrA and recB overlap by 8 nucleotides recB and recD overlap by 1 nucleotides
HEPCGN_19300: ptrA - pitrilysin, at 2,611,529 to 2,614,417
ptrA
HEPCGN_19305: recB - exodeoxyribonuclease V subunit beta, at 2,614,410 to 2,617,952
recB
HEPCGN_19310: recD - exodeoxyribonuclease V subunit alpha, at 2,617,952 to 2,619,778
recD
Position (kb)
2614
2615
2616
2617
2618 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2613.450 kb on - strand, within ptrA at 2613.452 kb on - strand, within ptrA at 2613.502 kb on + strand, within ptrA at 2613.503 kb on - strand, within ptrA at 2613.529 kb on - strand, within ptrA at 2613.529 kb on - strand, within ptrA at 2613.529 kb on - strand, within ptrA at 2613.533 kb on + strand, within ptrA at 2613.534 kb on - strand, within ptrA at 2613.575 kb on - strand, within ptrA at 2613.634 kb on + strand, within ptrA at 2613.738 kb on + strand, within ptrA at 2613.739 kb on - strand, within ptrA at 2613.739 kb on - strand, within ptrA at 2613.739 kb on - strand, within ptrA at 2613.816 kb on + strand, within ptrA at 2613.836 kb on + strand, within ptrA at 2613.853 kb on + strand, within ptrA at 2613.854 kb on - strand, within ptrA at 2613.854 kb on - strand, within ptrA at 2613.854 kb on - strand, within ptrA at 2613.876 kb on + strand, within ptrA at 2613.876 kb on + strand, within ptrA at 2613.876 kb on + strand, within ptrA at 2613.890 kb on + strand, within ptrA at 2613.891 kb on - strand, within ptrA at 2613.905 kb on - strand, within ptrA at 2614.007 kb on + strand, within ptrA at 2614.022 kb on + strand, within ptrA at 2614.022 kb on + strand, within ptrA at 2614.022 kb on + strand, within ptrA at 2614.101 kb on + strand, within ptrA at 2614.180 kb on + strand at 2614.181 kb on - strand at 2614.218 kb on + strand at 2614.282 kb on + strand at 2614.283 kb on - strand at 2614.432 kb on + strand at 2614.432 kb on + strand at 2614.432 kb on + strand at 2614.433 kb on - strand at 2614.519 kb on - strand at 2614.532 kb on - strand at 2614.640 kb on + strand at 2614.699 kb on - strand at 2615.105 kb on - strand, within recB at 2615.247 kb on + strand, within recB at 2615.404 kb on + strand, within recB at 2615.498 kb on + strand, within recB at 2615.606 kb on + strand, within recB at 2615.663 kb on + strand, within recB at 2615.664 kb on - strand, within recB at 2615.667 kb on + strand, within recB at 2615.698 kb on - strand, within recB at 2615.699 kb on + strand, within recB at 2615.699 kb on + strand, within recB at 2615.737 kb on + strand, within recB at 2615.737 kb on + strand, within recB at 2615.738 kb on - strand, within recB at 2615.835 kb on - strand, within recB at 2615.869 kb on + strand, within recB at 2615.957 kb on + strand, within recB at 2615.999 kb on - strand, within recB at 2615.999 kb on - strand, within recB at 2616.552 kb on - strand, within recB at 2616.569 kb on + strand, within recB at 2616.605 kb on + strand, within recB at 2617.001 kb on + strand, within recB at 2617.525 kb on + strand, within recB at 2617.583 kb on - strand, within recB at 2617.594 kb on + strand, within recB at 2617.638 kb on + strand at 2617.651 kb on - strand at 2617.652 kb on + strand at 2617.751 kb on + strand at 2617.752 kb on - strand at 2617.770 kb on + strand at 2617.794 kb on + strand at 2617.795 kb on - strand at 2617.827 kb on + strand at 2617.827 kb on + strand at 2617.875 kb on + strand at 2617.971 kb on + strand at 2617.999 kb on - strand at 2618.076 kb on + strand at 2618.077 kb on - strand at 2618.077 kb on - strand at 2618.273 kb on + strand, within recD at 2618.274 kb on - strand, within recD at 2618.404 kb on + strand, within recD at 2618.405 kb on - strand, within recD at 2618.405 kb on - strand, within recD at 2618.515 kb on - strand, within recD at 2618.665 kb on + strand, within recD at 2618.710 kb on - strand, within recD at 2618.897 kb on + strand, within recD at 2618.937 kb on + strand, within recD at 2618.938 kb on - strand, within recD at 2618.938 kb on - strand, within recD at 2618.938 kb on - strand, within recD
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 2,613,450 - ptrA HEPCGN_19300 0.66 -2.1 2,613,452 - ptrA HEPCGN_19300 0.67 +0.3 2,613,502 + ptrA HEPCGN_19300 0.68 +1.4 2,613,503 - ptrA HEPCGN_19300 0.68 +0.6 2,613,529 - ptrA HEPCGN_19300 0.69 +0.6 2,613,529 - ptrA HEPCGN_19300 0.69 -0.5 2,613,529 - ptrA HEPCGN_19300 0.69 +0.4 2,613,533 + ptrA HEPCGN_19300 0.69 +1.1 2,613,534 - ptrA HEPCGN_19300 0.69 +0.5 2,613,575 - ptrA HEPCGN_19300 0.71 +0.2 2,613,634 + ptrA HEPCGN_19300 0.73 -0.2 2,613,738 + ptrA HEPCGN_19300 0.76 +0.2 2,613,739 - ptrA HEPCGN_19300 0.76 +0.0 2,613,739 - ptrA HEPCGN_19300 0.76 -3.5 2,613,739 - ptrA HEPCGN_19300 0.76 -0.2 2,613,816 + ptrA HEPCGN_19300 0.79 -0.5 2,613,836 + ptrA HEPCGN_19300 0.80 +0.2 2,613,853 + ptrA HEPCGN_19300 0.80 -0.1 2,613,854 - ptrA HEPCGN_19300 0.80 -0.6 2,613,854 - ptrA HEPCGN_19300 0.80 +0.1 2,613,854 - ptrA HEPCGN_19300 0.80 -0.0 2,613,876 + ptrA HEPCGN_19300 0.81 -1.1 2,613,876 + ptrA HEPCGN_19300 0.81 -1.7 2,613,876 + ptrA HEPCGN_19300 0.81 -1.1 2,613,890 + ptrA HEPCGN_19300 0.82 -1.7 2,613,891 - ptrA HEPCGN_19300 0.82 +0.1 2,613,905 - ptrA HEPCGN_19300 0.82 -0.0 2,614,007 + ptrA HEPCGN_19300 0.86 -0.8 2,614,022 + ptrA HEPCGN_19300 0.86 +0.5 2,614,022 + ptrA HEPCGN_19300 0.86 +0.3 2,614,022 + ptrA HEPCGN_19300 0.86 -2.5 2,614,101 + ptrA HEPCGN_19300 0.89 +0.5 2,614,180 + -0.7 2,614,181 - +0.9 2,614,218 + -0.1 2,614,282 + +0.7 2,614,283 - -2.0 2,614,432 + +0.5 2,614,432 + -1.9 2,614,432 + -1.2 2,614,433 - -1.8 2,614,519 - -0.9 2,614,532 - +0.4 2,614,640 + +1.4 2,614,699 - -1.9 2,615,105 - recB HEPCGN_19305 0.20 -0.9 2,615,247 + recB HEPCGN_19305 0.24 -0.7 2,615,404 + recB HEPCGN_19305 0.28 -1.9 2,615,498 + recB HEPCGN_19305 0.31 -0.2 2,615,606 + recB HEPCGN_19305 0.34 -0.9 2,615,663 + recB HEPCGN_19305 0.35 -2.3 2,615,664 - recB HEPCGN_19305 0.35 -1.4 2,615,667 + recB HEPCGN_19305 0.35 -1.9 2,615,698 - recB HEPCGN_19305 0.36 -0.6 2,615,699 + recB HEPCGN_19305 0.36 -1.6 2,615,699 + recB HEPCGN_19305 0.36 -1.2 2,615,737 + recB HEPCGN_19305 0.37 -1.6 2,615,737 + recB HEPCGN_19305 0.37 -2.3 2,615,738 - recB HEPCGN_19305 0.37 +0.4 2,615,835 - recB HEPCGN_19305 0.40 -0.7 2,615,869 + recB HEPCGN_19305 0.41 -0.6 2,615,957 + recB HEPCGN_19305 0.44 -1.0 2,615,999 - recB HEPCGN_19305 0.45 +0.4 2,615,999 - recB HEPCGN_19305 0.45 -3.4 2,616,552 - recB HEPCGN_19305 0.60 -2.1 2,616,569 + recB HEPCGN_19305 0.61 -2.2 2,616,605 + recB HEPCGN_19305 0.62 -0.2 2,617,001 + recB HEPCGN_19305 0.73 -2.4 2,617,525 + recB HEPCGN_19305 0.88 -2.4 2,617,583 - recB HEPCGN_19305 0.90 -1.2 2,617,594 + recB HEPCGN_19305 0.90 -1.6 2,617,638 + -2.5 2,617,651 - -0.8 2,617,652 + -0.9 2,617,751 + -1.4 2,617,752 - -1.6 2,617,770 + -0.2 2,617,794 + +0.4 2,617,795 - -0.8 2,617,827 + -1.9 2,617,827 + -0.6 2,617,875 + +0.4 2,617,971 + -0.0 2,617,999 - -1.2 2,618,076 + -1.0 2,618,077 - -0.6 2,618,077 - -0.2 2,618,273 + recD HEPCGN_19310 0.18 +0.0 2,618,274 - recD HEPCGN_19310 0.18 -1.6 2,618,404 + recD HEPCGN_19310 0.25 +0.1 2,618,405 - recD HEPCGN_19310 0.25 -0.5 2,618,405 - recD HEPCGN_19310 0.25 -1.3 2,618,515 - recD HEPCGN_19310 0.31 -0.4 2,618,665 + recD HEPCGN_19310 0.39 -2.3 2,618,710 - recD HEPCGN_19310 0.41 +0.0 2,618,897 + recD HEPCGN_19310 0.52 -0.8 2,618,937 + recD HEPCGN_19310 0.54 +1.0 2,618,938 - recD HEPCGN_19310 0.54 -0.1 2,618,938 - recD HEPCGN_19310 0.54 -0.8 2,618,938 - recD HEPCGN_19310 0.54 -0.7
Or see this region's nucleotide sequence