Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00720 and MMJJ_RS00725 are separated by 62 nucleotides MMJJ_RS00725 and MMJJ_RS00730 are separated by 253 nucleotides MMJJ_RS00730 and MMJJ_RS00735 are separated by 73 nucleotides MMJJ_RS00735 and MMJJ_RS00740 are separated by 48 nucleotides
MMJJ_RS00720: MMJJ_RS00720 - APC family permease, at 139,929 to 141,182
_RS00720
MMJJ_RS00725: MMJJ_RS00725 - LysE family translocator, at 141,245 to 141,868
_RS00725
MMJJ_RS00730: MMJJ_RS00730 - peptide-methionine (S)-S-oxide reductase MsrA, at 142,122 to 142,595
_RS00730
MMJJ_RS00735: MMJJ_RS00735 - hypothetical protein, at 142,669 to 143,268
_RS00735
MMJJ_RS00740: MMJJ_RS00740 - hypothetical protein, at 143,317 to 143,586
_RS00740
Position (kb) 142
143 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 141.155 kb on - strand at 141.155 kb on - strand at 141.155 kb on - strand at 141.156 kb on + strand at 141.156 kb on + strand at 141.156 kb on + strand at 141.158 kb on + strand at 141.183 kb on + strand at 141.194 kb on - strand at 141.204 kb on - strand at 141.205 kb on + strand at 141.209 kb on + strand at 141.228 kb on + strand at 141.232 kb on - strand at 141.243 kb on - strand at 141.243 kb on - strand at 141.247 kb on + strand at 141.288 kb on + strand at 141.312 kb on - strand, within MMJJ_RS00725 at 141.313 kb on + strand, within MMJJ_RS00725 at 141.313 kb on + strand, within MMJJ_RS00725 at 141.365 kb on - strand, within MMJJ_RS00725 at 141.366 kb on + strand, within MMJJ_RS00725 at 141.387 kb on + strand, within MMJJ_RS00725 at 141.419 kb on - strand, within MMJJ_RS00725 at 141.423 kb on + strand, within MMJJ_RS00725 at 141.510 kb on + strand, within MMJJ_RS00725 at 141.572 kb on - strand, within MMJJ_RS00725 at 141.575 kb on - strand, within MMJJ_RS00725 at 141.613 kb on + strand, within MMJJ_RS00725 at 141.621 kb on - strand, within MMJJ_RS00725 at 141.641 kb on - strand, within MMJJ_RS00725 at 141.649 kb on - strand, within MMJJ_RS00725 at 141.669 kb on - strand, within MMJJ_RS00725 at 141.713 kb on - strand, within MMJJ_RS00725 at 141.713 kb on - strand, within MMJJ_RS00725 at 141.714 kb on + strand, within MMJJ_RS00725 at 141.715 kb on - strand, within MMJJ_RS00725 at 141.715 kb on - strand, within MMJJ_RS00725 at 141.715 kb on - strand, within MMJJ_RS00725 at 141.715 kb on - strand, within MMJJ_RS00725 at 141.716 kb on + strand, within MMJJ_RS00725 at 141.716 kb on + strand, within MMJJ_RS00725 at 141.716 kb on + strand, within MMJJ_RS00725 at 141.724 kb on - strand, within MMJJ_RS00725 at 141.772 kb on - strand, within MMJJ_RS00725 at 141.785 kb on - strand, within MMJJ_RS00725 at 141.786 kb on + strand, within MMJJ_RS00725 at 141.786 kb on + strand, within MMJJ_RS00725 at 141.791 kb on - strand, within MMJJ_RS00725 at 141.792 kb on + strand, within MMJJ_RS00725 at 141.794 kb on - strand, within MMJJ_RS00725 at 141.794 kb on - strand, within MMJJ_RS00725 at 141.794 kb on - strand, within MMJJ_RS00725 at 141.795 kb on + strand, within MMJJ_RS00725 at 141.818 kb on + strand at 141.818 kb on + strand at 141.857 kb on + strand at 141.857 kb on + strand at 141.891 kb on - strand at 141.899 kb on - strand at 141.899 kb on - strand at 141.899 kb on - strand at 141.900 kb on + strand at 141.903 kb on - strand at 141.904 kb on + strand at 141.904 kb on + strand at 141.904 kb on + strand at 141.912 kb on - strand at 141.913 kb on + strand at 141.973 kb on - strand at 141.974 kb on + strand at 141.974 kb on + strand at 141.992 kb on - strand at 141.992 kb on - strand at 141.993 kb on + strand at 141.993 kb on + strand at 141.993 kb on + strand at 141.993 kb on + strand at 142.004 kb on - strand at 142.005 kb on + strand at 142.005 kb on + strand at 142.005 kb on + strand at 142.008 kb on - strand at 142.009 kb on + strand at 142.043 kb on + strand at 142.061 kb on - strand at 142.077 kb on + strand at 142.077 kb on + strand at 142.077 kb on + strand at 142.096 kb on - strand at 142.097 kb on + strand at 142.100 kb on + strand at 142.100 kb on + strand at 142.101 kb on - strand at 142.181 kb on + strand, within MMJJ_RS00730 at 142.202 kb on + strand, within MMJJ_RS00730 at 142.252 kb on + strand, within MMJJ_RS00730 at 142.284 kb on + strand, within MMJJ_RS00730 at 142.292 kb on + strand, within MMJJ_RS00730 at 142.306 kb on + strand, within MMJJ_RS00730 at 142.318 kb on - strand, within MMJJ_RS00730 at 142.318 kb on - strand, within MMJJ_RS00730 at 142.318 kb on - strand, within MMJJ_RS00730 at 142.318 kb on - strand, within MMJJ_RS00730 at 142.319 kb on + strand, within MMJJ_RS00730 at 142.326 kb on - strand, within MMJJ_RS00730 at 142.326 kb on - strand, within MMJJ_RS00730 at 142.327 kb on + strand, within MMJJ_RS00730 at 142.327 kb on + strand, within MMJJ_RS00730 at 142.327 kb on + strand, within MMJJ_RS00730 at 142.327 kb on + strand, within MMJJ_RS00730 at 142.340 kb on + strand, within MMJJ_RS00730 at 142.356 kb on + strand at 142.370 kb on - strand, within MMJJ_RS00730 at 142.407 kb on - strand, within MMJJ_RS00730 at 142.417 kb on + strand, within MMJJ_RS00730 at 142.419 kb on + strand, within MMJJ_RS00730 at 142.425 kb on - strand, within MMJJ_RS00730 at 142.447 kb on - strand, within MMJJ_RS00730 at 142.485 kb on + strand, within MMJJ_RS00730 at 142.499 kb on + strand, within MMJJ_RS00730 at 142.530 kb on - strand, within MMJJ_RS00730 at 142.557 kb on - strand at 142.574 kb on - strand at 142.665 kb on - strand at 142.666 kb on + strand at 142.666 kb on + strand at 142.704 kb on + strand at 142.732 kb on - strand, within MMJJ_RS00735 at 142.836 kb on + strand, within MMJJ_RS00735 at 142.836 kb on + strand, within MMJJ_RS00735 at 142.839 kb on + strand, within MMJJ_RS00735 at 142.841 kb on - strand, within MMJJ_RS00735 at 142.841 kb on - strand, within MMJJ_RS00735 at 142.842 kb on + strand, within MMJJ_RS00735 at 142.847 kb on - strand, within MMJJ_RS00735 at 142.889 kb on - strand, within MMJJ_RS00735 at 142.926 kb on - strand at 142.964 kb on - strand, within MMJJ_RS00735 at 142.964 kb on - strand, within MMJJ_RS00735 at 142.964 kb on - strand, within MMJJ_RS00735 at 142.964 kb on - strand, within MMJJ_RS00735 at 142.964 kb on - strand, within MMJJ_RS00735 at 142.964 kb on - strand, within MMJJ_RS00735 at 142.965 kb on + strand, within MMJJ_RS00735 at 142.965 kb on + strand, within MMJJ_RS00735 at 142.965 kb on + strand, within MMJJ_RS00735 at 142.965 kb on + strand, within MMJJ_RS00735 at 142.965 kb on + strand, within MMJJ_RS00735 at 142.965 kb on + strand, within MMJJ_RS00735 at 142.965 kb on + strand, within MMJJ_RS00735 at 142.965 kb on + strand, within MMJJ_RS00735 at 142.990 kb on - strand, within MMJJ_RS00735 at 143.007 kb on - strand, within MMJJ_RS00735 at 143.007 kb on - strand, within MMJJ_RS00735 at 143.035 kb on + strand, within MMJJ_RS00735 at 143.086 kb on + strand, within MMJJ_RS00735 at 143.086 kb on + strand, within MMJJ_RS00735 at 143.089 kb on + strand, within MMJJ_RS00735 at 143.090 kb on - strand, within MMJJ_RS00735 at 143.091 kb on + strand, within MMJJ_RS00735 at 143.138 kb on + strand, within MMJJ_RS00735 at 143.166 kb on + strand, within MMJJ_RS00735 at 143.222 kb on + strand at 143.222 kb on + strand at 143.288 kb on + strand at 143.314 kb on + strand at 143.314 kb on + strand at 143.345 kb on + strand, within MMJJ_RS00740 at 143.406 kb on - strand, within MMJJ_RS00740 at 143.406 kb on - strand, within MMJJ_RS00740 at 143.410 kb on - strand, within MMJJ_RS00740 at 143.413 kb on - strand, within MMJJ_RS00740 at 143.414 kb on + strand, within MMJJ_RS00740 at 143.416 kb on - strand, within MMJJ_RS00740 at 143.416 kb on - strand, within MMJJ_RS00740 at 143.416 kb on - strand, within MMJJ_RS00740 at 143.416 kb on - strand, within MMJJ_RS00740 at 143.416 kb on - strand, within MMJJ_RS00740 at 143.417 kb on + strand, within MMJJ_RS00740 at 143.417 kb on + strand, within MMJJ_RS00740 at 143.432 kb on - strand, within MMJJ_RS00740 at 143.432 kb on - strand, within MMJJ_RS00740 at 143.433 kb on + strand, within MMJJ_RS00740 at 143.443 kb on - strand, within MMJJ_RS00740 at 143.465 kb on - strand, within MMJJ_RS00740 at 143.466 kb on + strand, within MMJJ_RS00740 at 143.507 kb on - strand, within MMJJ_RS00740 at 143.508 kb on + strand, within MMJJ_RS00740 at 143.517 kb on - strand, within MMJJ_RS00740 at 143.518 kb on + strand, within MMJJ_RS00740 at 143.527 kb on - strand, within MMJJ_RS00740 at 143.527 kb on - strand, within MMJJ_RS00740 at 143.556 kb on + strand, within MMJJ_RS00740
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE remove 141,155 - -0.7 141,155 - +0.4 141,155 - +0.5 141,156 + +0.4 141,156 + -0.7 141,156 + -0.7 141,158 + -0.8 141,183 + -0.1 141,194 - +0.9 141,204 - -1.0 141,205 + -1.3 141,209 + -1.2 141,228 + -1.6 141,232 - -0.6 141,243 - +0.2 141,243 - -0.2 141,247 + -0.5 141,288 + +0.6 141,312 - MMJJ_RS00725 0.11 +2.1 141,313 + MMJJ_RS00725 0.11 +0.3 141,313 + MMJJ_RS00725 0.11 -0.8 141,365 - MMJJ_RS00725 0.19 +0.3 141,366 + MMJJ_RS00725 0.19 -0.7 141,387 + MMJJ_RS00725 0.23 +0.1 141,419 - MMJJ_RS00725 0.28 +0.5 141,423 + MMJJ_RS00725 0.29 -2.1 141,510 + MMJJ_RS00725 0.42 +0.7 141,572 - MMJJ_RS00725 0.52 +1.9 141,575 - MMJJ_RS00725 0.53 +0.5 141,613 + MMJJ_RS00725 0.59 -0.9 141,621 - MMJJ_RS00725 0.60 +0.5 141,641 - MMJJ_RS00725 0.63 +2.4 141,649 - MMJJ_RS00725 0.65 +0.8 141,669 - MMJJ_RS00725 0.68 +0.1 141,713 - MMJJ_RS00725 0.75 +0.7 141,713 - MMJJ_RS00725 0.75 +1.5 141,714 + MMJJ_RS00725 0.75 -1.1 141,715 - MMJJ_RS00725 0.75 +2.1 141,715 - MMJJ_RS00725 0.75 +0.1 141,715 - MMJJ_RS00725 0.75 +2.3 141,715 - MMJJ_RS00725 0.75 +0.5 141,716 + MMJJ_RS00725 0.75 -0.2 141,716 + MMJJ_RS00725 0.75 -1.0 141,716 + MMJJ_RS00725 0.75 -0.1 141,724 - MMJJ_RS00725 0.77 -1.0 141,772 - MMJJ_RS00725 0.84 -0.9 141,785 - MMJJ_RS00725 0.87 +2.0 141,786 + MMJJ_RS00725 0.87 -1.1 141,786 + MMJJ_RS00725 0.87 +1.2 141,791 - MMJJ_RS00725 0.88 +0.1 141,792 + MMJJ_RS00725 0.88 -1.5 141,794 - MMJJ_RS00725 0.88 +0.6 141,794 - MMJJ_RS00725 0.88 +0.1 141,794 - MMJJ_RS00725 0.88 -0.7 141,795 + MMJJ_RS00725 0.88 +0.2 141,818 + +0.1 141,818 + +0.6 141,857 + +0.2 141,857 + -0.7 141,891 - +1.3 141,899 - +0.1 141,899 - -1.3 141,899 - +0.2 141,900 + +0.8 141,903 - +0.3 141,904 + -0.1 141,904 + -0.9 141,904 + +1.8 141,912 - +0.6 141,913 + +0.3 141,973 - -0.5 141,974 + -0.1 141,974 + -1.6 141,992 - -0.9 141,992 - +0.9 141,993 + -1.8 141,993 + -0.8 141,993 + +0.4 141,993 + +1.0 142,004 - +0.1 142,005 + +0.2 142,005 + -1.2 142,005 + +0.7 142,008 - -1.8 142,009 + -0.7 142,043 + +0.8 142,061 - -0.7 142,077 + -0.9 142,077 + -1.2 142,077 + -1.2 142,096 - +1.2 142,097 + +1.4 142,100 + -0.9 142,100 + +0.7 142,101 - -0.7 142,181 + MMJJ_RS00730 0.12 -2.3 142,202 + MMJJ_RS00730 0.17 +0.9 142,252 + MMJJ_RS00730 0.27 -2.0 142,284 + MMJJ_RS00730 0.34 +0.5 142,292 + MMJJ_RS00730 0.36 -0.6 142,306 + MMJJ_RS00730 0.39 -1.4 142,318 - MMJJ_RS00730 0.41 +1.4 142,318 - MMJJ_RS00730 0.41 +0.3 142,318 - MMJJ_RS00730 0.41 -0.7 142,318 - MMJJ_RS00730 0.41 +0.6 142,319 + MMJJ_RS00730 0.42 -1.0 142,326 - MMJJ_RS00730 0.43 -1.4 142,326 - MMJJ_RS00730 0.43 -0.8 142,327 + MMJJ_RS00730 0.43 -2.3 142,327 + MMJJ_RS00730 0.43 -0.6 142,327 + MMJJ_RS00730 0.43 -0.6 142,327 + MMJJ_RS00730 0.43 +4.9 142,340 + MMJJ_RS00730 0.46 +0.6 142,356 + +2.0 142,370 - MMJJ_RS00730 0.52 +1.6 142,407 - MMJJ_RS00730 0.60 +0.3 142,417 + MMJJ_RS00730 0.62 +0.4 142,419 + MMJJ_RS00730 0.63 -0.9 142,425 - MMJJ_RS00730 0.64 +0.5 142,447 - MMJJ_RS00730 0.69 +1.1 142,485 + MMJJ_RS00730 0.77 -1.6 142,499 + MMJJ_RS00730 0.80 -0.7 142,530 - MMJJ_RS00730 0.86 +0.4 142,557 - +1.7 142,574 - -1.2 142,665 - +0.4 142,666 + -1.3 142,666 + -0.6 142,704 + +1.8 142,732 - MMJJ_RS00735 0.10 +0.8 142,836 + MMJJ_RS00735 0.28 -0.3 142,836 + MMJJ_RS00735 0.28 +1.2 142,839 + MMJJ_RS00735 0.28 -1.7 142,841 - MMJJ_RS00735 0.29 +1.4 142,841 - MMJJ_RS00735 0.29 +0.5 142,842 + MMJJ_RS00735 0.29 +1.1 142,847 - MMJJ_RS00735 0.30 +0.4 142,889 - MMJJ_RS00735 0.37 -0.7 142,926 - -0.6 142,964 - MMJJ_RS00735 0.49 -1.3 142,964 - MMJJ_RS00735 0.49 +0.5 142,964 - MMJJ_RS00735 0.49 -1.1 142,964 - MMJJ_RS00735 0.49 +0.3 142,964 - MMJJ_RS00735 0.49 -0.7 142,964 - MMJJ_RS00735 0.49 +0.5 142,965 + MMJJ_RS00735 0.49 -1.0 142,965 + MMJJ_RS00735 0.49 +1.0 142,965 + MMJJ_RS00735 0.49 +0.1 142,965 + MMJJ_RS00735 0.49 +0.4 142,965 + MMJJ_RS00735 0.49 +0.7 142,965 + MMJJ_RS00735 0.49 +0.1 142,965 + MMJJ_RS00735 0.49 -1.3 142,965 + MMJJ_RS00735 0.49 +0.5 142,990 - MMJJ_RS00735 0.54 -0.9 143,007 - MMJJ_RS00735 0.56 +1.6 143,007 - MMJJ_RS00735 0.56 -0.1 143,035 + MMJJ_RS00735 0.61 -0.5 143,086 + MMJJ_RS00735 0.69 +0.5 143,086 + MMJJ_RS00735 0.69 -0.9 143,089 + MMJJ_RS00735 0.70 +1.9 143,090 - MMJJ_RS00735 0.70 +1.1 143,091 + MMJJ_RS00735 0.70 -1.6 143,138 + MMJJ_RS00735 0.78 +0.3 143,166 + MMJJ_RS00735 0.83 +0.2 143,222 + +1.4 143,222 + +1.1 143,288 + -2.1 143,314 + +1.4 143,314 + -0.2 143,345 + MMJJ_RS00740 0.10 -1.1 143,406 - MMJJ_RS00740 0.33 +1.0 143,406 - MMJJ_RS00740 0.33 +0.6 143,410 - MMJJ_RS00740 0.34 +2.0 143,413 - MMJJ_RS00740 0.36 +0.4 143,414 + MMJJ_RS00740 0.36 +0.3 143,416 - MMJJ_RS00740 0.37 -0.5 143,416 - MMJJ_RS00740 0.37 -1.0 143,416 - MMJJ_RS00740 0.37 -1.9 143,416 - MMJJ_RS00740 0.37 -1.2 143,416 - MMJJ_RS00740 0.37 -1.5 143,417 + MMJJ_RS00740 0.37 -0.7 143,417 + MMJJ_RS00740 0.37 -1.0 143,432 - MMJJ_RS00740 0.43 +0.5 143,432 - MMJJ_RS00740 0.43 -1.3 143,433 + MMJJ_RS00740 0.43 +2.4 143,443 - MMJJ_RS00740 0.47 -2.2 143,465 - MMJJ_RS00740 0.55 +0.3 143,466 + MMJJ_RS00740 0.55 -1.0 143,507 - MMJJ_RS00740 0.70 +0.6 143,508 + MMJJ_RS00740 0.71 -0.4 143,517 - MMJJ_RS00740 0.74 +0.4 143,518 + MMJJ_RS00740 0.74 -1.2 143,527 - MMJJ_RS00740 0.78 +0.4 143,527 - MMJJ_RS00740 0.78 +0.6 143,556 + MMJJ_RS00740 0.89 -0.8
Or see this region's nucleotide sequence