Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16255 and RR42_RS16260 are separated by 11 nucleotides RR42_RS16260 and RR42_RS16265 overlap by 4 nucleotides RR42_RS16265 and RR42_RS16270 are separated by 17 nucleotides
RR42_RS16255: RR42_RS16255 - sulfate adenylyltransferase, at 3,518,915 to 3,519,877
_RS16255
RR42_RS16260: RR42_RS16260 - phosphoadenosine phosphosulfate reductase, at 3,519,889 to 3,520,677
_RS16260
RR42_RS16265: RR42_RS16265 - required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) , at 3,520,674 to 3,521,255
_RS16265
RR42_RS16270: RR42_RS16270 - sulfite reductase, at 3,521,273 to 3,522,952
_RS16270
Position (kb)
3519
3520
3521 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3518.916 kb on + strand at 3518.922 kb on + strand at 3518.923 kb on - strand at 3519.199 kb on - strand, within RR42_RS16255 at 3519.234 kb on + strand, within RR42_RS16255 at 3519.234 kb on + strand, within RR42_RS16255 at 3519.235 kb on - strand, within RR42_RS16255 at 3519.312 kb on + strand, within RR42_RS16255 at 3519.313 kb on - strand, within RR42_RS16255 at 3519.313 kb on - strand, within RR42_RS16255 at 3519.634 kb on + strand, within RR42_RS16255 at 3519.634 kb on + strand, within RR42_RS16255 at 3519.635 kb on - strand, within RR42_RS16255 at 3519.780 kb on + strand, within RR42_RS16255 at 3519.780 kb on + strand, within RR42_RS16255 at 3519.781 kb on - strand at 3519.781 kb on - strand at 3519.993 kb on + strand, within RR42_RS16260 at 3520.068 kb on - strand, within RR42_RS16260 at 3520.751 kb on - strand, within RR42_RS16265 at 3520.918 kb on + strand, within RR42_RS16265 at 3520.919 kb on - strand, within RR42_RS16265 at 3521.244 kb on - strand at 3521.261 kb on + strand at 3521.388 kb on + strand at 3521.388 kb on + strand at 3521.388 kb on + strand at 3521.389 kb on - strand at 3521.389 kb on - strand at 3521.389 kb on - strand at 3521.522 kb on - strand, within RR42_RS16270 at 3521.588 kb on - strand, within RR42_RS16270 at 3521.588 kb on - strand, within RR42_RS16270 at 3521.619 kb on + strand, within RR42_RS16270 at 3521.620 kb on - strand, within RR42_RS16270
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 3,518,916 + -5.6 3,518,922 + -3.9 3,518,923 - -3.3 3,519,199 - RR42_RS16255 0.29 -6.4 3,519,234 + RR42_RS16255 0.33 -2.5 3,519,234 + RR42_RS16255 0.33 -1.5 3,519,235 - RR42_RS16255 0.33 -3.5 3,519,312 + RR42_RS16255 0.41 -3.6 3,519,313 - RR42_RS16255 0.41 -4.3 3,519,313 - RR42_RS16255 0.41 -3.9 3,519,634 + RR42_RS16255 0.75 -3.1 3,519,634 + RR42_RS16255 0.75 -3.3 3,519,635 - RR42_RS16255 0.75 -5.3 3,519,780 + RR42_RS16255 0.90 -3.6 3,519,780 + RR42_RS16255 0.90 -4.8 3,519,781 - -2.1 3,519,781 - -4.5 3,519,993 + RR42_RS16260 0.13 -4.3 3,520,068 - RR42_RS16260 0.23 -1.4 3,520,751 - RR42_RS16265 0.13 -5.5 3,520,918 + RR42_RS16265 0.42 -3.6 3,520,919 - RR42_RS16265 0.42 -5.0 3,521,244 - -3.1 3,521,261 + -1.2 3,521,388 + -3.1 3,521,388 + -5.5 3,521,388 + -2.4 3,521,389 - -2.6 3,521,389 - -3.8 3,521,389 - -4.6 3,521,522 - RR42_RS16270 0.15 -4.0 3,521,588 - RR42_RS16270 0.19 -4.3 3,521,588 - RR42_RS16270 0.19 -3.9 3,521,619 + RR42_RS16270 0.21 -3.7 3,521,620 - RR42_RS16270 0.21 -3.7
Or see this region's nucleotide sequence