Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04105 and MMJJ_RS04110 are separated by 13 nucleotides MMJJ_RS04110 and MMJJ_RS04115 are separated by 81 nucleotides MMJJ_RS04115 and MMJJ_RS04120 are separated by 40 nucleotides MMJJ_RS04120 and MMJJ_RS04125 are separated by 28 nucleotides
MMJJ_RS04105: MMJJ_RS04105 - prepilin peptidase, at 761,839 to 762,879
_RS04105
MMJJ_RS04110: MMJJ_RS04110 - hypothetical protein, at 762,893 to 763,261
_RS04110
MMJJ_RS04115: MMJJ_RS04115 - HDIG domain-containing protein, at 763,343 to 764,029
_RS04115
MMJJ_RS04120: MMJJ_RS04120 - amino acid ABC transporter ATP-binding protein, at 764,070 to 764,801
_RS04120
MMJJ_RS04125: MMJJ_RS04125 - tRNA (guanine(10)-N(2))-dimethyltransferase, at 764,830 to 765,951
_RS04125
Position (kb) 763
764
765 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 762.348 kb on - strand, within MMJJ_RS04105 at 762.349 kb on + strand, within MMJJ_RS04105 at 762.379 kb on + strand, within MMJJ_RS04105 at 762.399 kb on + strand, within MMJJ_RS04105 at 762.430 kb on + strand, within MMJJ_RS04105 at 762.434 kb on - strand, within MMJJ_RS04105 at 762.434 kb on - strand, within MMJJ_RS04105 at 762.437 kb on + strand, within MMJJ_RS04105 at 762.480 kb on + strand, within MMJJ_RS04105 at 762.480 kb on + strand, within MMJJ_RS04105 at 762.499 kb on + strand, within MMJJ_RS04105 at 762.499 kb on + strand, within MMJJ_RS04105 at 762.560 kb on + strand, within MMJJ_RS04105 at 762.560 kb on + strand, within MMJJ_RS04105 at 762.572 kb on + strand, within MMJJ_RS04105 at 762.580 kb on + strand, within MMJJ_RS04105 at 762.596 kb on - strand, within MMJJ_RS04105 at 762.596 kb on - strand, within MMJJ_RS04105 at 762.597 kb on + strand, within MMJJ_RS04105 at 762.597 kb on + strand, within MMJJ_RS04105 at 762.597 kb on + strand, within MMJJ_RS04105 at 762.598 kb on - strand, within MMJJ_RS04105 at 762.598 kb on - strand, within MMJJ_RS04105 at 762.599 kb on + strand, within MMJJ_RS04105 at 762.600 kb on - strand, within MMJJ_RS04105 at 762.600 kb on - strand, within MMJJ_RS04105 at 762.600 kb on - strand, within MMJJ_RS04105 at 762.600 kb on - strand, within MMJJ_RS04105 at 762.601 kb on + strand, within MMJJ_RS04105 at 762.625 kb on - strand, within MMJJ_RS04105 at 762.625 kb on - strand, within MMJJ_RS04105 at 762.625 kb on - strand, within MMJJ_RS04105 at 762.677 kb on + strand, within MMJJ_RS04105 at 762.677 kb on + strand, within MMJJ_RS04105 at 762.682 kb on - strand, within MMJJ_RS04105 at 762.683 kb on + strand, within MMJJ_RS04105 at 762.683 kb on + strand, within MMJJ_RS04105 at 762.710 kb on + strand, within MMJJ_RS04105 at 762.745 kb on - strand, within MMJJ_RS04105 at 762.746 kb on + strand, within MMJJ_RS04105 at 762.750 kb on - strand, within MMJJ_RS04105 at 762.757 kb on - strand, within MMJJ_RS04105 at 762.758 kb on + strand, within MMJJ_RS04105 at 762.763 kb on - strand, within MMJJ_RS04105 at 762.764 kb on + strand, within MMJJ_RS04105 at 762.766 kb on - strand, within MMJJ_RS04105 at 762.766 kb on - strand, within MMJJ_RS04105 at 762.766 kb on - strand, within MMJJ_RS04105 at 762.767 kb on + strand, within MMJJ_RS04105 at 762.767 kb on + strand, within MMJJ_RS04105 at 762.768 kb on - strand, within MMJJ_RS04105 at 762.768 kb on - strand, within MMJJ_RS04105 at 762.769 kb on - strand, within MMJJ_RS04105 at 762.807 kb on - strand at 762.807 kb on - strand at 762.807 kb on - strand at 762.807 kb on - strand at 762.807 kb on - strand at 762.808 kb on + strand at 762.808 kb on + strand at 762.809 kb on - strand at 762.809 kb on - strand at 762.809 kb on - strand at 762.809 kb on - strand at 762.809 kb on - strand at 762.809 kb on - strand at 762.809 kb on - strand at 762.809 kb on - strand at 762.809 kb on - strand at 762.810 kb on + strand at 762.810 kb on + strand at 762.810 kb on + strand at 762.810 kb on + strand at 762.810 kb on + strand at 762.810 kb on + strand at 762.824 kb on + strand at 762.837 kb on - strand at 762.841 kb on - strand at 762.853 kb on - strand at 762.865 kb on + strand at 762.904 kb on - strand at 762.905 kb on + strand at 762.938 kb on - strand, within MMJJ_RS04110 at 762.939 kb on + strand, within MMJJ_RS04110 at 762.941 kb on + strand, within MMJJ_RS04110 at 762.941 kb on + strand, within MMJJ_RS04110 at 762.947 kb on - strand, within MMJJ_RS04110 at 762.961 kb on - strand, within MMJJ_RS04110 at 762.986 kb on - strand at 762.986 kb on - strand at 762.986 kb on - strand, within MMJJ_RS04110 at 762.986 kb on - strand, within MMJJ_RS04110 at 762.986 kb on - strand, within MMJJ_RS04110 at 762.987 kb on + strand, within MMJJ_RS04110 at 762.987 kb on + strand, within MMJJ_RS04110 at 762.995 kb on + strand, within MMJJ_RS04110 at 763.004 kb on + strand, within MMJJ_RS04110 at 763.022 kb on - strand, within MMJJ_RS04110 at 763.069 kb on - strand, within MMJJ_RS04110 at 763.104 kb on + strand, within MMJJ_RS04110 at 763.138 kb on + strand, within MMJJ_RS04110 at 763.176 kb on - strand, within MMJJ_RS04110 at 763.192 kb on + strand, within MMJJ_RS04110 at 763.235 kb on - strand at 763.251 kb on + strand at 763.257 kb on + strand at 763.261 kb on - strand at 763.261 kb on - strand at 763.261 kb on - strand at 763.261 kb on - strand at 763.261 kb on - strand at 763.261 kb on - strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.262 kb on + strand at 763.263 kb on - strand at 763.263 kb on - strand at 763.263 kb on - strand at 763.263 kb on - strand at 763.264 kb on + strand at 763.264 kb on + strand at 763.264 kb on + strand at 763.264 kb on + strand at 763.264 kb on + strand at 763.264 kb on + strand at 763.280 kb on - strand at 763.280 kb on - strand at 763.292 kb on - strand at 763.292 kb on - strand at 763.292 kb on - strand at 763.292 kb on - strand at 763.292 kb on - strand at 763.292 kb on - strand at 763.293 kb on + strand at 763.293 kb on + strand at 763.293 kb on + strand at 763.293 kb on + strand at 763.294 kb on - strand at 763.294 kb on - strand at 763.295 kb on + strand at 763.295 kb on + strand at 763.295 kb on + strand at 763.295 kb on + strand at 763.297 kb on + strand at 763.297 kb on + strand at 763.297 kb on + strand at 763.304 kb on - strand at 763.304 kb on - strand at 763.304 kb on - strand at 763.304 kb on - strand at 763.305 kb on + strand at 763.309 kb on - strand at 763.309 kb on - strand at 763.310 kb on + strand at 763.310 kb on + strand at 763.321 kb on - strand at 763.321 kb on - strand at 763.321 kb on - strand at 763.321 kb on - strand at 763.322 kb on + strand at 763.323 kb on - strand at 763.324 kb on + strand at 763.324 kb on + strand at 763.324 kb on + strand at 763.325 kb on - strand at 763.325 kb on - strand at 763.325 kb on - strand at 763.341 kb on - strand at 763.341 kb on - strand at 763.376 kb on + strand at 763.403 kb on + strand at 763.410 kb on + strand at 763.410 kb on + strand at 763.411 kb on - strand at 763.412 kb on + strand, within MMJJ_RS04115 at 763.414 kb on + strand, within MMJJ_RS04115 at 763.414 kb on + strand, within MMJJ_RS04115 at 763.414 kb on + strand, within MMJJ_RS04115 at 763.417 kb on - strand, within MMJJ_RS04115 at 763.417 kb on - strand, within MMJJ_RS04115 at 763.417 kb on - strand, within MMJJ_RS04115 at 763.459 kb on + strand, within MMJJ_RS04115 at 763.499 kb on + strand, within MMJJ_RS04115 at 763.499 kb on + strand, within MMJJ_RS04115 at 763.499 kb on + strand, within MMJJ_RS04115 at 763.499 kb on + strand, within MMJJ_RS04115 at 763.525 kb on - strand, within MMJJ_RS04115 at 763.537 kb on - strand, within MMJJ_RS04115 at 763.594 kb on + strand, within MMJJ_RS04115 at 763.594 kb on + strand, within MMJJ_RS04115 at 763.636 kb on - strand, within MMJJ_RS04115 at 763.659 kb on - strand, within MMJJ_RS04115 at 763.707 kb on - strand, within MMJJ_RS04115 at 763.767 kb on - strand, within MMJJ_RS04115 at 763.797 kb on - strand, within MMJJ_RS04115 at 763.797 kb on - strand, within MMJJ_RS04115 at 763.845 kb on - strand, within MMJJ_RS04115 at 763.845 kb on - strand, within MMJJ_RS04115 at 763.845 kb on - strand, within MMJJ_RS04115 at 763.846 kb on + strand, within MMJJ_RS04115 at 763.846 kb on + strand, within MMJJ_RS04115 at 763.859 kb on + strand, within MMJJ_RS04115 at 763.867 kb on + strand, within MMJJ_RS04115 at 763.878 kb on - strand, within MMJJ_RS04115 at 763.879 kb on + strand, within MMJJ_RS04115 at 763.894 kb on - strand, within MMJJ_RS04115 at 764.119 kb on - strand at 764.129 kb on + strand at 764.159 kb on + strand, within MMJJ_RS04120 at 764.201 kb on - strand, within MMJJ_RS04120 at 764.240 kb on - strand, within MMJJ_RS04120 at 764.261 kb on - strand, within MMJJ_RS04120 at 764.328 kb on + strand, within MMJJ_RS04120 at 764.363 kb on - strand, within MMJJ_RS04120 at 764.363 kb on - strand, within MMJJ_RS04120 at 764.363 kb on - strand, within MMJJ_RS04120 at 764.387 kb on + strand, within MMJJ_RS04120 at 764.428 kb on - strand, within MMJJ_RS04120 at 764.428 kb on - strand, within MMJJ_RS04120 at 764.429 kb on + strand, within MMJJ_RS04120 at 764.431 kb on - strand, within MMJJ_RS04120 at 764.546 kb on + strand, within MMJJ_RS04120 at 764.546 kb on + strand, within MMJJ_RS04120 at 764.594 kb on + strand, within MMJJ_RS04120 at 764.602 kb on - strand, within MMJJ_RS04120 at 764.602 kb on - strand, within MMJJ_RS04120 at 764.602 kb on - strand, within MMJJ_RS04120 at 764.644 kb on - strand, within MMJJ_RS04120 at 764.741 kb on - strand at 764.741 kb on - strand at 764.742 kb on + strand at 764.751 kb on + strand at 764.751 kb on + strand at 764.795 kb on - strand at 764.795 kb on - strand at 764.796 kb on + strand at 764.799 kb on - strand at 764.799 kb on - strand at 764.799 kb on - strand at 764.800 kb on + strand at 764.800 kb on + strand at 764.802 kb on - strand at 764.815 kb on + strand at 764.820 kb on - strand at 764.820 kb on - strand at 764.820 kb on - strand at 764.925 kb on - strand at 764.931 kb on - strand at 764.962 kb on + strand, within MMJJ_RS04125 at 764.992 kb on + strand, within MMJJ_RS04125
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 762,348 - MMJJ_RS04105 0.49 -2.7 762,349 + MMJJ_RS04105 0.49 -3.0 762,379 + MMJJ_RS04105 0.52 -0.8 762,399 + MMJJ_RS04105 0.54 -1.4 762,430 + MMJJ_RS04105 0.57 +1.2 762,434 - MMJJ_RS04105 0.57 +0.3 762,434 - MMJJ_RS04105 0.57 +0.1 762,437 + MMJJ_RS04105 0.57 -0.4 762,480 + MMJJ_RS04105 0.62 -0.3 762,480 + MMJJ_RS04105 0.62 -2.1 762,499 + MMJJ_RS04105 0.63 -1.5 762,499 + MMJJ_RS04105 0.63 +0.3 762,560 + MMJJ_RS04105 0.69 +1.4 762,560 + MMJJ_RS04105 0.69 -0.3 762,572 + MMJJ_RS04105 0.70 -2.0 762,580 + MMJJ_RS04105 0.71 -0.6 762,596 - MMJJ_RS04105 0.73 +0.8 762,596 - MMJJ_RS04105 0.73 -0.7 762,597 + MMJJ_RS04105 0.73 -2.8 762,597 + MMJJ_RS04105 0.73 +1.8 762,597 + MMJJ_RS04105 0.73 +0.1 762,598 - MMJJ_RS04105 0.73 -2.9 762,598 - MMJJ_RS04105 0.73 -0.5 762,599 + MMJJ_RS04105 0.73 -2.9 762,600 - MMJJ_RS04105 0.73 -2.5 762,600 - MMJJ_RS04105 0.73 -0.1 762,600 - MMJJ_RS04105 0.73 -3.4 762,600 - MMJJ_RS04105 0.73 -0.7 762,601 + MMJJ_RS04105 0.73 -2.1 762,625 - MMJJ_RS04105 0.76 -0.1 762,625 - MMJJ_RS04105 0.76 -0.1 762,625 - MMJJ_RS04105 0.76 +1.9 762,677 + MMJJ_RS04105 0.80 +0.7 762,677 + MMJJ_RS04105 0.80 -0.6 762,682 - MMJJ_RS04105 0.81 -1.7 762,683 + MMJJ_RS04105 0.81 +1.0 762,683 + MMJJ_RS04105 0.81 -0.6 762,710 + MMJJ_RS04105 0.84 -1.0 762,745 - MMJJ_RS04105 0.87 -0.3 762,746 + MMJJ_RS04105 0.87 -0.0 762,750 - MMJJ_RS04105 0.88 -0.8 762,757 - MMJJ_RS04105 0.88 -2.3 762,758 + MMJJ_RS04105 0.88 +1.2 762,763 - MMJJ_RS04105 0.89 -0.0 762,764 + MMJJ_RS04105 0.89 -1.8 762,766 - MMJJ_RS04105 0.89 +0.5 762,766 - MMJJ_RS04105 0.89 -0.3 762,766 - MMJJ_RS04105 0.89 +0.4 762,767 + MMJJ_RS04105 0.89 -3.7 762,767 + MMJJ_RS04105 0.89 -0.1 762,768 - MMJJ_RS04105 0.89 -0.8 762,768 - MMJJ_RS04105 0.89 +0.0 762,769 - MMJJ_RS04105 0.89 -3.6 762,807 - -3.4 762,807 - +0.7 762,807 - +1.1 762,807 - +0.1 762,807 - -4.3 762,808 + +1.2 762,808 + +1.6 762,809 - -2.0 762,809 - -0.1 762,809 - -1.7 762,809 - +0.8 762,809 - -5.3 762,809 - -2.3 762,809 - -1.0 762,809 - +0.3 762,809 - -1.5 762,810 + +0.5 762,810 + -1.5 762,810 + -4.9 762,810 + -3.3 762,810 + -3.5 762,810 + -2.3 762,824 + +0.5 762,837 - +0.2 762,841 - -0.2 762,853 - -0.3 762,865 + -1.0 762,904 - +0.4 762,905 + +0.5 762,938 - MMJJ_RS04110 0.12 -0.0 762,939 + MMJJ_RS04110 0.12 +0.0 762,941 + MMJJ_RS04110 0.13 +2.4 762,941 + MMJJ_RS04110 0.13 -1.2 762,947 - MMJJ_RS04110 0.15 -0.8 762,961 - MMJJ_RS04110 0.18 -1.0 762,986 - +1.6 762,986 - +0.6 762,986 - MMJJ_RS04110 0.25 +1.6 762,986 - MMJJ_RS04110 0.25 -1.0 762,986 - MMJJ_RS04110 0.25 +0.6 762,987 + MMJJ_RS04110 0.25 +0.6 762,987 + MMJJ_RS04110 0.25 -2.0 762,995 + MMJJ_RS04110 0.28 -1.2 763,004 + MMJJ_RS04110 0.30 +1.6 763,022 - MMJJ_RS04110 0.35 -1.0 763,069 - MMJJ_RS04110 0.48 +1.6 763,104 + MMJJ_RS04110 0.57 +0.6 763,138 + MMJJ_RS04110 0.66 +0.6 763,176 - MMJJ_RS04110 0.77 -0.4 763,192 + MMJJ_RS04110 0.81 -0.0 763,235 - +0.6 763,251 + +1.6 763,257 + +0.6 763,261 - -1.4 763,261 - +0.6 763,261 - -0.0 763,261 - +1.6 763,261 - -0.4 763,261 - -0.0 763,262 + -0.8 763,262 + +0.6 763,262 + -2.1 763,262 + -0.0 763,262 + -0.4 763,262 + -0.0 763,262 + -1.6 763,262 + +0.6 763,262 + -0.0 763,263 - -0.4 763,263 - -0.0 763,263 - +0.6 763,263 - +0.6 763,264 + -0.4 763,264 + -2.3 763,264 + -2.5 763,264 + -2.1 763,264 + -1.8 763,264 + +1.6 763,280 - -0.0 763,280 - +0.6 763,292 - -1.6 763,292 - -0.5 763,292 - +1.6 763,292 - -1.6 763,292 - +1.6 763,292 - +1.1 763,293 + +1.5 763,293 + -0.8 763,293 + -2.5 763,293 + -2.3 763,294 - +2.3 763,294 - +1.7 763,295 + -0.3 763,295 + +0.6 763,295 + +1.0 763,295 + +1.0 763,297 + -0.0 763,297 + -3.6 763,297 + +0.4 763,304 - -1.5 763,304 - +0.6 763,304 - +0.2 763,304 - -0.8 763,305 + +0.8 763,309 - -0.1 763,309 - +0.6 763,310 + +0.9 763,310 + +0.7 763,321 - +1.4 763,321 - -0.4 763,321 - +2.6 763,321 - +1.3 763,322 + +1.1 763,323 - +3.4 763,324 + +2.2 763,324 + +1.5 763,324 + +0.6 763,325 - -4.4 763,325 - -0.7 763,325 - +1.2 763,341 - +0.8 763,341 - +1.5 763,376 + +1.2 763,403 + +1.0 763,410 + -0.5 763,410 + -1.9 763,411 - +1.3 763,412 + MMJJ_RS04115 0.10 +1.4 763,414 + MMJJ_RS04115 0.10 -2.9 763,414 + MMJJ_RS04115 0.10 -0.8 763,414 + MMJJ_RS04115 0.10 -1.2 763,417 - MMJJ_RS04115 0.11 -1.3 763,417 - MMJJ_RS04115 0.11 +2.4 763,417 - MMJJ_RS04115 0.11 -0.3 763,459 + MMJJ_RS04115 0.17 +1.1 763,499 + MMJJ_RS04115 0.23 +3.9 763,499 + MMJJ_RS04115 0.23 -2.8 763,499 + MMJJ_RS04115 0.23 -0.1 763,499 + MMJJ_RS04115 0.23 +0.7 763,525 - MMJJ_RS04115 0.26 +1.3 763,537 - MMJJ_RS04115 0.28 +0.8 763,594 + MMJJ_RS04115 0.37 -0.6 763,594 + MMJJ_RS04115 0.37 +0.8 763,636 - MMJJ_RS04115 0.43 +0.2 763,659 - MMJJ_RS04115 0.46 +0.1 763,707 - MMJJ_RS04115 0.53 +1.9 763,767 - MMJJ_RS04115 0.62 -1.7 763,797 - MMJJ_RS04115 0.66 +0.4 763,797 - MMJJ_RS04115 0.66 +3.8 763,845 - MMJJ_RS04115 0.73 -0.1 763,845 - MMJJ_RS04115 0.73 -0.9 763,845 - MMJJ_RS04115 0.73 +1.0 763,846 + MMJJ_RS04115 0.73 +1.3 763,846 + MMJJ_RS04115 0.73 -3.6 763,859 + MMJJ_RS04115 0.75 -0.3 763,867 + MMJJ_RS04115 0.76 +0.8 763,878 - MMJJ_RS04115 0.78 +1.3 763,879 + MMJJ_RS04115 0.78 +1.2 763,894 - MMJJ_RS04115 0.80 -1.1 764,119 - +1.8 764,129 + -1.8 764,159 + MMJJ_RS04120 0.12 +1.7 764,201 - MMJJ_RS04120 0.18 +1.2 764,240 - MMJJ_RS04120 0.23 -1.2 764,261 - MMJJ_RS04120 0.26 +0.3 764,328 + MMJJ_RS04120 0.35 +2.4 764,363 - MMJJ_RS04120 0.40 -2.2 764,363 - MMJJ_RS04120 0.40 -0.1 764,363 - MMJJ_RS04120 0.40 -2.8 764,387 + MMJJ_RS04120 0.43 -0.3 764,428 - MMJJ_RS04120 0.49 +0.2 764,428 - MMJJ_RS04120 0.49 +0.3 764,429 + MMJJ_RS04120 0.49 +1.1 764,431 - MMJJ_RS04120 0.49 +1.1 764,546 + MMJJ_RS04120 0.65 -2.7 764,546 + MMJJ_RS04120 0.65 -1.5 764,594 + MMJJ_RS04120 0.72 -0.0 764,602 - MMJJ_RS04120 0.73 +1.5 764,602 - MMJJ_RS04120 0.73 -0.3 764,602 - MMJJ_RS04120 0.73 -0.4 764,644 - MMJJ_RS04120 0.78 +1.8 764,741 - +0.9 764,741 - +0.3 764,742 + +0.9 764,751 + -2.1 764,751 + +0.6 764,795 - -0.1 764,795 - +1.1 764,796 + +0.1 764,799 - +1.2 764,799 - -1.2 764,799 - -0.3 764,800 + -0.7 764,800 + +0.6 764,802 - +1.1 764,815 + -0.3 764,820 - -0.2 764,820 - +0.1 764,820 - +0.3 764,925 - -0.7 764,931 - -3.3 764,962 + MMJJ_RS04125 0.12 -2.1 764,992 + MMJJ_RS04125 0.14 -1.3
Or see this region's nucleotide sequence