Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01115 and MMJJ_RS01120 are separated by 199 nucleotides MMJJ_RS01120 and MMJJ_RS01125 are separated by 9 nucleotides MMJJ_RS01125 and MMJJ_RS09255 are separated by 170 nucleotides MMJJ_RS09255 and MMJJ_RS01135 are separated by 64 nucleotides
MMJJ_RS01115: MMJJ_RS01115 - hypothetical protein, at 217,014 to 217,340
_RS01115
MMJJ_RS01120: MMJJ_RS01120 - hypothetical protein, at 217,540 to 217,821
_RS01120
MMJJ_RS01125: MMJJ_RS01125 - hypothetical protein, at 217,831 to 218,205
_RS01125
MMJJ_RS09255: MMJJ_RS09255 - hypothetical protein, at 218,376 to 218,561
_RS09255
MMJJ_RS01135: MMJJ_RS01135 - hypothetical protein, at 218,626 to 222,324
_RS01135
Position (kb) 217
218
219 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 216.866 kb on + strand at 216.960 kb on - strand at 216.960 kb on - strand at 216.961 kb on + strand at 216.962 kb on - strand at 217.121 kb on - strand, within MMJJ_RS01115 at 217.213 kb on - strand, within MMJJ_RS01115 at 217.215 kb on - strand, within MMJJ_RS01115 at 217.215 kb on - strand, within MMJJ_RS01115 at 217.215 kb on - strand, within MMJJ_RS01115 at 217.216 kb on + strand, within MMJJ_RS01115 at 217.270 kb on - strand, within MMJJ_RS01115 at 217.281 kb on - strand, within MMJJ_RS01115 at 217.281 kb on - strand, within MMJJ_RS01115 at 217.282 kb on + strand, within MMJJ_RS01115 at 217.282 kb on + strand, within MMJJ_RS01115 at 217.283 kb on - strand, within MMJJ_RS01115 at 217.283 kb on - strand, within MMJJ_RS01115 at 217.335 kb on + strand at 217.469 kb on + strand at 217.469 kb on + strand at 217.469 kb on + strand at 217.472 kb on + strand at 217.472 kb on + strand at 217.474 kb on - strand at 217.480 kb on - strand at 217.480 kb on - strand at 217.480 kb on - strand at 217.481 kb on + strand at 217.481 kb on + strand at 217.482 kb on - strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.484 kb on - strand at 217.484 kb on - strand at 217.484 kb on - strand at 217.484 kb on - strand at 217.484 kb on - strand at 217.485 kb on + strand at 217.485 kb on + strand at 217.485 kb on + strand at 217.485 kb on + strand at 217.485 kb on + strand at 217.486 kb on - strand at 217.486 kb on - strand at 217.486 kb on - strand at 217.486 kb on - strand at 217.486 kb on - strand at 217.487 kb on + strand at 217.487 kb on + strand at 217.487 kb on + strand at 217.487 kb on + strand at 217.578 kb on - strand, within MMJJ_RS01120 at 217.579 kb on + strand, within MMJJ_RS01120 at 217.580 kb on - strand, within MMJJ_RS01120 at 217.581 kb on + strand, within MMJJ_RS01120 at 217.599 kb on - strand, within MMJJ_RS01120 at 217.600 kb on + strand, within MMJJ_RS01120 at 217.607 kb on - strand, within MMJJ_RS01120 at 217.616 kb on - strand, within MMJJ_RS01120 at 217.616 kb on - strand, within MMJJ_RS01120 at 217.616 kb on - strand, within MMJJ_RS01120 at 217.616 kb on - strand, within MMJJ_RS01120 at 217.616 kb on - strand, within MMJJ_RS01120 at 217.617 kb on + strand, within MMJJ_RS01120 at 217.617 kb on + strand, within MMJJ_RS01120 at 217.617 kb on + strand, within MMJJ_RS01120 at 217.624 kb on - strand, within MMJJ_RS01120 at 217.624 kb on - strand, within MMJJ_RS01120 at 217.624 kb on - strand, within MMJJ_RS01120 at 217.625 kb on + strand at 217.625 kb on + strand, within MMJJ_RS01120 at 217.626 kb on - strand, within MMJJ_RS01120 at 217.626 kb on - strand, within MMJJ_RS01120 at 217.626 kb on - strand, within MMJJ_RS01120 at 217.626 kb on - strand, within MMJJ_RS01120 at 217.626 kb on - strand, within MMJJ_RS01120 at 217.627 kb on + strand, within MMJJ_RS01120 at 217.627 kb on + strand, within MMJJ_RS01120 at 217.628 kb on - strand, within MMJJ_RS01120 at 217.628 kb on - strand, within MMJJ_RS01120 at 217.667 kb on + strand, within MMJJ_RS01120 at 217.686 kb on - strand, within MMJJ_RS01120 at 217.686 kb on - strand, within MMJJ_RS01120 at 217.687 kb on + strand, within MMJJ_RS01120 at 217.687 kb on + strand, within MMJJ_RS01120 at 217.687 kb on + strand, within MMJJ_RS01120 at 217.687 kb on + strand, within MMJJ_RS01120 at 217.693 kb on - strand, within MMJJ_RS01120 at 217.694 kb on + strand, within MMJJ_RS01120 at 217.712 kb on + strand, within MMJJ_RS01120 at 217.712 kb on + strand, within MMJJ_RS01120 at 217.722 kb on + strand, within MMJJ_RS01120 at 217.733 kb on + strand, within MMJJ_RS01120 at 217.733 kb on + strand, within MMJJ_RS01120 at 217.774 kb on - strand, within MMJJ_RS01120 at 217.775 kb on + strand, within MMJJ_RS01120 at 217.780 kb on + strand, within MMJJ_RS01120 at 217.819 kb on - strand at 217.820 kb on + strand at 217.851 kb on - strand at 217.851 kb on - strand at 217.851 kb on - strand at 217.852 kb on + strand at 217.855 kb on + strand at 218.067 kb on + strand, within MMJJ_RS01125 at 218.089 kb on - strand, within MMJJ_RS01125 at 218.091 kb on - strand, within MMJJ_RS01125 at 218.094 kb on - strand, within MMJJ_RS01125 at 218.095 kb on + strand, within MMJJ_RS01125 at 218.095 kb on + strand, within MMJJ_RS01125 at 218.095 kb on + strand, within MMJJ_RS01125 at 218.096 kb on - strand, within MMJJ_RS01125 at 218.144 kb on - strand, within MMJJ_RS01125 at 218.216 kb on + strand at 218.216 kb on + strand at 218.241 kb on - strand at 218.242 kb on + strand at 218.242 kb on + strand at 218.244 kb on - strand at 218.250 kb on - strand at 218.258 kb on - strand at 218.314 kb on - strand at 218.315 kb on + strand at 218.377 kb on - strand at 218.379 kb on - strand at 218.380 kb on + strand at 218.412 kb on + strand, within MMJJ_RS09255 at 218.414 kb on - strand, within MMJJ_RS09255 at 218.414 kb on - strand, within MMJJ_RS09255 at 218.442 kb on - strand, within MMJJ_RS09255 at 218.502 kb on + strand, within MMJJ_RS09255 at 218.634 kb on - strand at 218.653 kb on - strand at 218.654 kb on + strand at 218.659 kb on + strand at 218.675 kb on + strand at 218.677 kb on + strand at 218.677 kb on + strand at 218.682 kb on - strand at 218.731 kb on + strand at 218.914 kb on + strand at 218.917 kb on - strand at 219.034 kb on - strand, within MMJJ_RS01135 at 219.109 kb on + strand, within MMJJ_RS01135 at 219.121 kb on + strand, within MMJJ_RS01135
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 216,866 + +2.3 216,960 - -1.6 216,960 - +2.0 216,961 + +1.2 216,962 - -0.7 217,121 - MMJJ_RS01115 0.33 -1.0 217,213 - MMJJ_RS01115 0.61 -1.4 217,215 - MMJJ_RS01115 0.61 +0.9 217,215 - MMJJ_RS01115 0.61 +0.6 217,215 - MMJJ_RS01115 0.61 +0.2 217,216 + MMJJ_RS01115 0.62 +0.3 217,270 - MMJJ_RS01115 0.78 +1.3 217,281 - MMJJ_RS01115 0.82 +2.1 217,281 - MMJJ_RS01115 0.82 +0.8 217,282 + MMJJ_RS01115 0.82 -1.3 217,282 + MMJJ_RS01115 0.82 +1.9 217,283 - MMJJ_RS01115 0.82 -0.7 217,283 - MMJJ_RS01115 0.82 -1.2 217,335 + -0.7 217,469 + -3.3 217,469 + +1.8 217,469 + -3.2 217,472 + +1.2 217,472 + -0.3 217,474 - -0.7 217,480 - +0.0 217,480 - +0.8 217,480 - -1.5 217,481 + -1.7 217,481 + -0.0 217,482 - -0.7 217,483 + -0.8 217,483 + +1.7 217,483 + -0.7 217,483 + +0.0 217,483 + +0.4 217,483 + +1.3 217,483 + -0.1 217,483 + +0.5 217,484 - -2.7 217,484 - +1.0 217,484 - -1.5 217,484 - +0.4 217,484 - -2.6 217,485 + -0.7 217,485 + -0.6 217,485 + +0.1 217,485 + +1.1 217,485 + -0.6 217,486 - +0.7 217,486 - +0.4 217,486 - -0.7 217,486 - -0.1 217,486 - -0.7 217,487 + -0.6 217,487 + -0.1 217,487 + +0.9 217,487 + -0.6 217,578 - MMJJ_RS01120 0.13 +0.4 217,579 + MMJJ_RS01120 0.14 -3.1 217,580 - MMJJ_RS01120 0.14 -2.9 217,581 + MMJJ_RS01120 0.15 -0.2 217,599 - MMJJ_RS01120 0.21 +2.1 217,600 + MMJJ_RS01120 0.21 -1.3 217,607 - MMJJ_RS01120 0.24 -0.3 217,616 - MMJJ_RS01120 0.27 +1.0 217,616 - MMJJ_RS01120 0.27 -0.8 217,616 - MMJJ_RS01120 0.27 +1.0 217,616 - MMJJ_RS01120 0.27 +0.6 217,616 - MMJJ_RS01120 0.27 +2.6 217,617 + MMJJ_RS01120 0.27 +0.5 217,617 + MMJJ_RS01120 0.27 -2.7 217,617 + MMJJ_RS01120 0.27 -1.7 217,624 - MMJJ_RS01120 0.30 -1.3 217,624 - MMJJ_RS01120 0.30 +1.0 217,624 - MMJJ_RS01120 0.30 +1.1 217,625 + +1.3 217,625 + MMJJ_RS01120 0.30 +0.9 217,626 - MMJJ_RS01120 0.30 -2.1 217,626 - MMJJ_RS01120 0.30 +0.2 217,626 - MMJJ_RS01120 0.30 -2.0 217,626 - MMJJ_RS01120 0.30 -0.1 217,626 - MMJJ_RS01120 0.30 -0.2 217,627 + MMJJ_RS01120 0.31 -1.4 217,627 + MMJJ_RS01120 0.31 +0.5 217,628 - MMJJ_RS01120 0.31 +2.7 217,628 - MMJJ_RS01120 0.31 +0.1 217,667 + MMJJ_RS01120 0.45 -2.7 217,686 - MMJJ_RS01120 0.52 -3.6 217,686 - MMJJ_RS01120 0.52 -1.9 217,687 + MMJJ_RS01120 0.52 +2.5 217,687 + MMJJ_RS01120 0.52 -0.2 217,687 + MMJJ_RS01120 0.52 +1.2 217,687 + MMJJ_RS01120 0.52 +1.3 217,693 - MMJJ_RS01120 0.54 -4.3 217,694 + MMJJ_RS01120 0.55 +1.3 217,712 + MMJJ_RS01120 0.61 +2.0 217,712 + MMJJ_RS01120 0.61 -2.1 217,722 + MMJJ_RS01120 0.65 +0.8 217,733 + MMJJ_RS01120 0.68 -3.2 217,733 + MMJJ_RS01120 0.68 -0.5 217,774 - MMJJ_RS01120 0.83 +2.0 217,775 + MMJJ_RS01120 0.83 -0.1 217,780 + MMJJ_RS01120 0.85 -0.3 217,819 - -2.0 217,820 + +1.6 217,851 - +0.6 217,851 - +3.0 217,851 - -1.1 217,852 + -3.2 217,855 + -1.3 218,067 + MMJJ_RS01125 0.63 +1.3 218,089 - MMJJ_RS01125 0.69 +1.4 218,091 - MMJJ_RS01125 0.69 -0.5 218,094 - MMJJ_RS01125 0.70 -2.5 218,095 + MMJJ_RS01125 0.70 +2.0 218,095 + MMJJ_RS01125 0.70 +0.4 218,095 + MMJJ_RS01125 0.70 +2.8 218,096 - MMJJ_RS01125 0.71 +0.5 218,144 - MMJJ_RS01125 0.83 +2.0 218,216 + -4.1 218,216 + +1.2 218,241 - +1.3 218,242 + -1.7 218,242 + -1.3 218,244 - -0.1 218,250 - +1.4 218,258 - +0.8 218,314 - -0.0 218,315 + -3.3 218,377 - +0.3 218,379 - +0.6 218,380 + -3.3 218,412 + MMJJ_RS09255 0.19 +0.3 218,414 - MMJJ_RS09255 0.20 +0.5 218,414 - MMJJ_RS09255 0.20 -0.0 218,442 - MMJJ_RS09255 0.35 +1.7 218,502 + MMJJ_RS09255 0.68 -2.7 218,634 - -0.1 218,653 - +2.1 218,654 + -0.7 218,659 + -2.4 218,675 + +0.1 218,677 + -0.7 218,677 + +0.7 218,682 - +1.0 218,731 + +0.6 218,914 + -1.5 218,917 - -1.7 219,034 - MMJJ_RS01135 0.11 -2.1 219,109 + MMJJ_RS01135 0.13 +1.4 219,121 + MMJJ_RS01135 0.13 +0.0
Or see this region's nucleotide sequence