Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09300 and BPHYT_RS09305 are separated by 87 nucleotides BPHYT_RS09305 and BPHYT_RS09310 are separated by 159 nucleotides BPHYT_RS09310 and BPHYT_RS09315 are separated by 44 nucleotides
BPHYT_RS09300: BPHYT_RS09300 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 2,109,091 to 2,109,570
_RS09300
BPHYT_RS09305: BPHYT_RS09305 - alkyl hydroperoxide reductase, at 2,109,658 to 2,110,206
_RS09305
BPHYT_RS09310: BPHYT_RS09310 - sensor histidine kinase, at 2,110,366 to 2,111,715
_RS09310
BPHYT_RS09315: BPHYT_RS09315 - chemotaxis protein CheY, at 2,111,760 to 2,112,485
_RS09315
Position (kb)
2110
2111
2112 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2109.893 kb on + strand, within BPHYT_RS09305 at 2110.067 kb on + strand, within BPHYT_RS09305 at 2110.068 kb on - strand, within BPHYT_RS09305 at 2110.068 kb on - strand, within BPHYT_RS09305 at 2110.188 kb on + strand at 2110.276 kb on + strand at 2110.276 kb on + strand at 2110.276 kb on + strand at 2110.277 kb on - strand at 2110.277 kb on - strand at 2110.529 kb on + strand, within BPHYT_RS09310 at 2110.529 kb on + strand, within BPHYT_RS09310 at 2110.530 kb on - strand, within BPHYT_RS09310 at 2110.667 kb on + strand, within BPHYT_RS09310 at 2111.426 kb on + strand, within BPHYT_RS09310 at 2111.427 kb on - strand, within BPHYT_RS09310 at 2111.427 kb on - strand, within BPHYT_RS09310 at 2112.386 kb on - strand, within BPHYT_RS09315 at 2112.507 kb on - strand at 2112.542 kb on - strand at 2112.597 kb on - strand at 2112.679 kb on - strand at 2112.681 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,109,893 + BPHYT_RS09305 0.43 +0.7 2,110,067 + BPHYT_RS09305 0.74 +0.5 2,110,068 - BPHYT_RS09305 0.75 -0.1 2,110,068 - BPHYT_RS09305 0.75 -0.7 2,110,188 + +0.1 2,110,276 + -0.0 2,110,276 + +0.2 2,110,276 + -0.4 2,110,277 - +2.0 2,110,277 - -0.5 2,110,529 + BPHYT_RS09310 0.12 -2.0 2,110,529 + BPHYT_RS09310 0.12 -0.4 2,110,530 - BPHYT_RS09310 0.12 -1.4 2,110,667 + BPHYT_RS09310 0.22 -0.2 2,111,426 + BPHYT_RS09310 0.79 -1.9 2,111,427 - BPHYT_RS09310 0.79 -3.0 2,111,427 - BPHYT_RS09310 0.79 -1.9 2,112,386 - BPHYT_RS09315 0.86 -2.8 2,112,507 - -1.4 2,112,542 - -3.5 2,112,597 - -5.7 2,112,679 - -6.7 2,112,681 - -4.1
Or see this region's nucleotide sequence