Experiment: L-Lysine 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygbI and ygbJ are separated by 195 nucleotides ygbJ and ygbK overlap by 4 nucleotides
b2735: ygbI - predicted DNA-binding transcriptional regulator (RefSeq), at 2,858,489 to 2,859,256
ygbI
b2736: ygbJ - predicted dehydrogenase, with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,859,452 to 2,860,360
ygbJ
b2737: ygbK - hypothetical protein (NCBI), at 2,860,357 to 2,861,523
ygbK
Position (kb)
2859
2860
2861 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2858.457 kb on + strand at 2858.457 kb on - strand at 2858.466 kb on - strand at 2858.471 kb on - strand at 2858.471 kb on - strand at 2858.473 kb on - strand at 2858.476 kb on - strand at 2858.514 kb on + strand at 2858.518 kb on + strand at 2858.568 kb on + strand, within ygbI at 2858.568 kb on + strand, within ygbI at 2858.618 kb on - strand, within ygbI at 2858.719 kb on + strand, within ygbI at 2858.822 kb on - strand, within ygbI at 2858.827 kb on - strand, within ygbI at 2858.827 kb on - strand, within ygbI at 2858.912 kb on - strand, within ygbI at 2859.114 kb on - strand, within ygbI at 2859.160 kb on - strand, within ygbI at 2859.528 kb on - strand at 2859.528 kb on - strand at 2859.539 kb on - strand at 2859.664 kb on + strand, within ygbJ at 2859.852 kb on - strand, within ygbJ at 2859.962 kb on - strand, within ygbJ at 2859.965 kb on + strand, within ygbJ at 2860.243 kb on + strand, within ygbJ at 2860.243 kb on + strand, within ygbJ at 2860.296 kb on + strand at 2860.464 kb on - strand at 2860.540 kb on - strand, within ygbK at 2860.598 kb on - strand, within ygbK at 2860.598 kb on - strand, within ygbK at 2860.657 kb on + strand, within ygbK at 2860.721 kb on + strand, within ygbK at 2860.847 kb on - strand, within ygbK at 2860.851 kb on + strand, within ygbK at 2860.895 kb on - strand, within ygbK at 2860.990 kb on + strand, within ygbK at 2860.990 kb on + strand, within ygbK at 2860.997 kb on + strand, within ygbK at 2860.998 kb on - strand, within ygbK at 2861.029 kb on - strand, within ygbK at 2861.044 kb on + strand, within ygbK at 2861.044 kb on + strand, within ygbK at 2861.080 kb on + strand, within ygbK at 2861.111 kb on + strand, within ygbK at 2861.166 kb on - strand, within ygbK at 2861.195 kb on + strand, within ygbK at 2861.301 kb on + strand, within ygbK at 2861.301 kb on + strand, within ygbK
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 200 mM remove 2,858,457 + -0.7 2,858,457 - +1.1 2,858,466 - +1.1 2,858,471 - -0.3 2,858,471 - +0.3 2,858,473 - +0.6 2,858,476 - +0.5 2,858,514 + -1.1 2,858,518 + -1.7 2,858,568 + ygbI b2735 0.10 +0.7 2,858,568 + ygbI b2735 0.10 -0.1 2,858,618 - ygbI b2735 0.17 -1.9 2,858,719 + ygbI b2735 0.30 +1.4 2,858,822 - ygbI b2735 0.43 -0.2 2,858,827 - ygbI b2735 0.44 -0.3 2,858,827 - ygbI b2735 0.44 +0.5 2,858,912 - ygbI b2735 0.55 -0.4 2,859,114 - ygbI b2735 0.81 -0.7 2,859,160 - ygbI b2735 0.87 +0.1 2,859,528 - -1.9 2,859,528 - +0.6 2,859,539 - -0.7 2,859,664 + ygbJ b2736 0.23 -0.1 2,859,852 - ygbJ b2736 0.44 -1.1 2,859,962 - ygbJ b2736 0.56 -0.6 2,859,965 + ygbJ b2736 0.56 -0.4 2,860,243 + ygbJ b2736 0.87 -0.8 2,860,243 + ygbJ b2736 0.87 -1.5 2,860,296 + -0.6 2,860,464 - +1.1 2,860,540 - ygbK b2737 0.16 -0.3 2,860,598 - ygbK b2737 0.21 -0.9 2,860,598 - ygbK b2737 0.21 +0.8 2,860,657 + ygbK b2737 0.26 -0.6 2,860,721 + ygbK b2737 0.31 +0.1 2,860,847 - ygbK b2737 0.42 +0.7 2,860,851 + ygbK b2737 0.42 -0.5 2,860,895 - ygbK b2737 0.46 -1.3 2,860,990 + ygbK b2737 0.54 -0.9 2,860,990 + ygbK b2737 0.54 +0.1 2,860,997 + ygbK b2737 0.55 +1.8 2,860,998 - ygbK b2737 0.55 +0.4 2,861,029 - ygbK b2737 0.58 -0.0 2,861,044 + ygbK b2737 0.59 +1.6 2,861,044 + ygbK b2737 0.59 -0.5 2,861,080 + ygbK b2737 0.62 -0.0 2,861,111 + ygbK b2737 0.65 +0.2 2,861,166 - ygbK b2737 0.69 +0.5 2,861,195 + ygbK b2737 0.72 -0.4 2,861,301 + ygbK b2737 0.81 +1.7 2,861,301 + ygbK b2737 0.81 -0.3
Or see this region's nucleotide sequence