Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04620 and MMJJ_RS09305 are separated by 25 nucleotides MMJJ_RS09305 and MMJJ_RS04625 are separated by 266 nucleotides MMJJ_RS04625 and MMJJ_RS04630 are separated by 381 nucleotides MMJJ_RS04630 and MMJJ_RS04635 are separated by 4 nucleotides
MMJJ_RS04620: MMJJ_RS04620 - homocitrate synthase family protein, at 860,538 to 861,698
_RS04620
MMJJ_RS09305: MMJJ_RS09305 - hypothetical protein, at 861,724 to 861,882
_RS09305
MMJJ_RS04625: MMJJ_RS04625 - SLC13 family permease, at 862,149 to 863,489
_RS04625
MMJJ_RS04630: MMJJ_RS04630 - 50S ribosomal protein L40e, at 863,871 to 864,014
_RS04630
MMJJ_RS04635: MMJJ_RS04635 - DUF367 family protein, at 864,019 to 864,534
_RS04635
Position (kb) 862
863
864 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 861.668 kb on - strand at 861.697 kb on + strand at 861.697 kb on + strand at 861.701 kb on - strand at 861.723 kb on - strand at 861.724 kb on + strand at 861.735 kb on - strand at 861.736 kb on + strand at 861.746 kb on - strand, within MMJJ_RS09305 at 861.746 kb on - strand, within MMJJ_RS09305 at 861.746 kb on - strand, within MMJJ_RS09305 at 861.746 kb on - strand, within MMJJ_RS09305 at 861.757 kb on + strand, within MMJJ_RS09305 at 861.757 kb on + strand, within MMJJ_RS09305 at 861.798 kb on - strand, within MMJJ_RS09305 at 861.810 kb on - strand, within MMJJ_RS09305 at 861.811 kb on + strand, within MMJJ_RS09305 at 861.811 kb on + strand, within MMJJ_RS09305 at 861.811 kb on + strand, within MMJJ_RS09305 at 861.818 kb on - strand, within MMJJ_RS09305 at 861.824 kb on - strand, within MMJJ_RS09305 at 861.825 kb on + strand, within MMJJ_RS09305 at 861.825 kb on + strand, within MMJJ_RS09305 at 861.827 kb on - strand, within MMJJ_RS09305 at 861.846 kb on + strand, within MMJJ_RS09305 at 861.851 kb on - strand, within MMJJ_RS09305 at 861.859 kb on - strand, within MMJJ_RS09305 at 861.860 kb on + strand, within MMJJ_RS09305 at 861.872 kb on - strand at 861.876 kb on + strand at 861.881 kb on + strand at 861.939 kb on + strand at 861.939 kb on + strand at 861.955 kb on - strand at 861.956 kb on + strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.964 kb on - strand at 861.965 kb on + strand at 861.965 kb on + strand at 861.965 kb on + strand at 861.965 kb on + strand at 861.965 kb on + strand at 861.968 kb on - strand at 861.968 kb on - strand at 861.968 kb on - strand at 861.968 kb on - strand at 861.968 kb on - strand at 861.969 kb on + strand at 861.969 kb on + strand at 861.996 kb on - strand at 861.996 kb on - strand at 861.997 kb on + strand at 861.997 kb on + strand at 861.997 kb on + strand at 861.997 kb on + strand at 862.000 kb on - strand at 862.000 kb on - strand at 862.000 kb on - strand at 862.000 kb on - strand at 862.001 kb on + strand at 862.001 kb on + strand at 862.001 kb on + strand at 862.042 kb on - strand at 862.042 kb on - strand at 862.042 kb on - strand at 862.042 kb on - strand at 862.042 kb on - strand at 862.042 kb on - strand at 862.043 kb on + strand at 862.043 kb on + strand at 862.049 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.053 kb on - strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.054 kb on + strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.055 kb on - strand at 862.056 kb on + strand at 862.056 kb on + strand at 862.056 kb on + strand at 862.056 kb on + strand at 862.056 kb on + strand at 862.058 kb on - strand at 862.059 kb on + strand at 862.062 kb on - strand at 862.150 kb on - strand at 862.189 kb on + strand at 862.235 kb on - strand at 862.260 kb on - strand at 862.261 kb on + strand at 862.261 kb on + strand at 862.293 kb on - strand, within MMJJ_RS04625 at 862.480 kb on - strand, within MMJJ_RS04625 at 862.481 kb on + strand, within MMJJ_RS04625 at 862.481 kb on + strand, within MMJJ_RS04625 at 862.508 kb on - strand, within MMJJ_RS04625 at 862.509 kb on + strand, within MMJJ_RS04625 at 862.623 kb on - strand, within MMJJ_RS04625 at 862.623 kb on - strand, within MMJJ_RS04625 at 862.623 kb on - strand, within MMJJ_RS04625 at 862.632 kb on + strand, within MMJJ_RS04625 at 862.632 kb on + strand, within MMJJ_RS04625 at 862.657 kb on + strand, within MMJJ_RS04625 at 862.668 kb on + strand, within MMJJ_RS04625 at 862.686 kb on - strand, within MMJJ_RS04625 at 862.689 kb on - strand, within MMJJ_RS04625 at 862.690 kb on + strand, within MMJJ_RS04625 at 862.700 kb on - strand, within MMJJ_RS04625 at 862.701 kb on + strand, within MMJJ_RS04625 at 862.701 kb on + strand, within MMJJ_RS04625 at 862.730 kb on - strand, within MMJJ_RS04625 at 862.730 kb on - strand, within MMJJ_RS04625 at 862.748 kb on - strand, within MMJJ_RS04625 at 862.748 kb on - strand, within MMJJ_RS04625 at 862.791 kb on - strand, within MMJJ_RS04625 at 862.817 kb on + strand, within MMJJ_RS04625 at 862.832 kb on - strand, within MMJJ_RS04625 at 862.836 kb on - strand, within MMJJ_RS04625 at 862.836 kb on - strand, within MMJJ_RS04625 at 862.836 kb on - strand, within MMJJ_RS04625 at 862.837 kb on + strand, within MMJJ_RS04625 at 862.837 kb on + strand, within MMJJ_RS04625 at 862.838 kb on - strand, within MMJJ_RS04625 at 862.838 kb on - strand, within MMJJ_RS04625 at 862.839 kb on + strand, within MMJJ_RS04625 at 862.839 kb on + strand, within MMJJ_RS04625 at 862.908 kb on - strand, within MMJJ_RS04625 at 862.909 kb on + strand, within MMJJ_RS04625 at 862.966 kb on - strand, within MMJJ_RS04625 at 862.966 kb on - strand, within MMJJ_RS04625 at 863.016 kb on - strand, within MMJJ_RS04625 at 863.016 kb on - strand, within MMJJ_RS04625 at 863.016 kb on - strand, within MMJJ_RS04625 at 863.017 kb on + strand, within MMJJ_RS04625 at 863.177 kb on - strand, within MMJJ_RS04625 at 863.178 kb on + strand, within MMJJ_RS04625 at 863.182 kb on + strand, within MMJJ_RS04625 at 863.196 kb on - strand, within MMJJ_RS04625 at 863.271 kb on - strand, within MMJJ_RS04625 at 863.271 kb on - strand, within MMJJ_RS04625 at 863.368 kb on + strand at 863.369 kb on - strand at 863.370 kb on + strand at 863.397 kb on - strand at 863.438 kb on - strand at 863.449 kb on + strand at 863.470 kb on + strand at 863.470 kb on + strand at 863.474 kb on - strand at 863.474 kb on - strand at 863.475 kb on + strand at 863.475 kb on + strand at 863.505 kb on + strand at 863.568 kb on - strand at 863.583 kb on - strand at 863.585 kb on - strand at 863.585 kb on - strand at 863.585 kb on - strand at 863.585 kb on - strand at 863.586 kb on + strand at 863.586 kb on + strand at 863.586 kb on + strand at 863.591 kb on - strand at 863.592 kb on + strand at 863.615 kb on + strand at 863.615 kb on + strand at 863.617 kb on - strand at 863.617 kb on - strand at 863.618 kb on + strand at 863.669 kb on - strand at 863.669 kb on - strand at 863.677 kb on - strand at 863.677 kb on - strand at 863.678 kb on + strand at 863.700 kb on - strand at 863.704 kb on - strand at 863.705 kb on + strand at 863.731 kb on + strand at 863.736 kb on - strand at 863.736 kb on - strand at 863.738 kb on - strand at 863.750 kb on - strand at 863.754 kb on - strand at 863.754 kb on - strand at 863.755 kb on + strand at 863.755 kb on + strand at 863.758 kb on + strand at 863.761 kb on + strand at 863.778 kb on - strand at 863.792 kb on + strand at 863.804 kb on - strand at 863.804 kb on - strand at 863.805 kb on + strand at 863.911 kb on - strand, within MMJJ_RS04630 at 864.015 kb on - strand at 864.182 kb on + strand, within MMJJ_RS04635 at 864.182 kb on + strand, within MMJJ_RS04635 at 864.186 kb on + strand, within MMJJ_RS04635 at 864.186 kb on + strand, within MMJJ_RS04635 at 864.199 kb on - strand, within MMJJ_RS04635 at 864.208 kb on - strand, within MMJJ_RS04635 at 864.208 kb on - strand, within MMJJ_RS04635 at 864.209 kb on + strand, within MMJJ_RS04635 at 864.213 kb on - strand, within MMJJ_RS04635 at 864.216 kb on - strand, within MMJJ_RS04635 at 864.444 kb on - strand, within MMJJ_RS04635 at 864.486 kb on - strand at 864.486 kb on - strand at 864.486 kb on - strand at 864.487 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 861,668 - -2.7 861,697 + +1.0 861,697 + +2.6 861,701 - +0.3 861,723 - -0.3 861,724 + -0.3 861,735 - -0.1 861,736 + -0.2 861,746 - MMJJ_RS09305 0.14 +0.3 861,746 - MMJJ_RS09305 0.14 -0.3 861,746 - MMJJ_RS09305 0.14 +0.4 861,746 - MMJJ_RS09305 0.14 +0.1 861,757 + MMJJ_RS09305 0.21 +0.7 861,757 + MMJJ_RS09305 0.21 +0.1 861,798 - MMJJ_RS09305 0.47 +0.1 861,810 - MMJJ_RS09305 0.54 +0.2 861,811 + MMJJ_RS09305 0.55 +0.9 861,811 + MMJJ_RS09305 0.55 -0.4 861,811 + MMJJ_RS09305 0.55 +0.2 861,818 - MMJJ_RS09305 0.59 +0.3 861,824 - MMJJ_RS09305 0.63 +0.3 861,825 + MMJJ_RS09305 0.64 -0.3 861,825 + MMJJ_RS09305 0.64 +0.1 861,827 - MMJJ_RS09305 0.65 +0.3 861,846 + MMJJ_RS09305 0.77 -0.2 861,851 - MMJJ_RS09305 0.80 -0.3 861,859 - MMJJ_RS09305 0.85 +0.3 861,860 + MMJJ_RS09305 0.86 -0.6 861,872 - -1.1 861,876 + -0.2 861,881 + +0.3 861,939 + +0.2 861,939 + +0.3 861,955 - +0.3 861,956 + +0.3 861,964 - +1.6 861,964 - -0.3 861,964 - -0.4 861,964 - +0.0 861,964 - +0.2 861,964 - -3.0 861,964 - -0.4 861,964 - -0.4 861,964 - -0.2 861,965 + +0.0 861,965 + +0.6 861,965 + +0.4 861,965 + +0.2 861,965 + +1.3 861,968 - +2.0 861,968 - +0.9 861,968 - -0.5 861,968 - -0.1 861,968 - +0.0 861,969 + +0.6 861,969 + +2.0 861,996 - -0.0 861,996 - +0.1 861,997 + -0.5 861,997 + -0.1 861,997 + -0.1 861,997 + -0.0 862,000 - -0.1 862,000 - +0.1 862,000 - -0.2 862,000 - -0.6 862,001 + -0.3 862,001 + +0.2 862,001 + -0.2 862,042 - -0.4 862,042 - -0.8 862,042 - -0.1 862,042 - +0.2 862,042 - -0.2 862,042 - -0.4 862,043 + +0.7 862,043 + -1.5 862,049 - +0.1 862,053 - +0.2 862,053 - -0.5 862,053 - -0.7 862,053 - +0.3 862,053 - -0.2 862,053 - +0.4 862,053 - +0.5 862,053 - -0.2 862,053 - -0.6 862,053 - -0.1 862,053 - +0.3 862,053 - +0.4 862,053 - -0.6 862,054 + +0.0 862,054 + -0.6 862,054 + +0.1 862,054 + -0.9 862,054 + +0.1 862,054 + +0.2 862,054 + -0.6 862,054 + +0.5 862,054 + +0.3 862,054 + -0.3 862,054 + -0.3 862,054 + -0.5 862,054 + -0.3 862,054 + -1.0 862,055 - +1.5 862,055 - +0.1 862,055 - +0.8 862,055 - -1.1 862,055 - +0.3 862,055 - -1.0 862,055 - -0.4 862,055 - +0.1 862,055 - -0.5 862,055 - +0.0 862,055 - +0.0 862,055 - -0.2 862,055 - +0.6 862,055 - -1.6 862,055 - +2.3 862,056 + +0.5 862,056 + +0.1 862,056 + -0.3 862,056 + -0.1 862,056 + +1.6 862,058 - +0.2 862,059 + +0.3 862,062 - +0.2 862,150 - -0.2 862,189 + +0.8 862,235 - -0.6 862,260 - -0.2 862,261 + -0.3 862,261 + -0.3 862,293 - MMJJ_RS04625 0.11 +0.1 862,480 - MMJJ_RS04625 0.25 -0.3 862,481 + MMJJ_RS04625 0.25 -0.9 862,481 + MMJJ_RS04625 0.25 +0.6 862,508 - MMJJ_RS04625 0.27 -0.3 862,509 + MMJJ_RS04625 0.27 -0.6 862,623 - MMJJ_RS04625 0.35 +0.1 862,623 - MMJJ_RS04625 0.35 +0.6 862,623 - MMJJ_RS04625 0.35 +0.2 862,632 + MMJJ_RS04625 0.36 +0.7 862,632 + MMJJ_RS04625 0.36 +0.4 862,657 + MMJJ_RS04625 0.38 -0.8 862,668 + MMJJ_RS04625 0.39 -0.3 862,686 - MMJJ_RS04625 0.40 +0.1 862,689 - MMJJ_RS04625 0.40 +0.8 862,690 + MMJJ_RS04625 0.40 +0.5 862,700 - MMJJ_RS04625 0.41 -1.3 862,701 + MMJJ_RS04625 0.41 -0.0 862,701 + MMJJ_RS04625 0.41 +0.7 862,730 - MMJJ_RS04625 0.43 +0.6 862,730 - MMJJ_RS04625 0.43 -0.7 862,748 - MMJJ_RS04625 0.45 -0.7 862,748 - MMJJ_RS04625 0.45 +0.5 862,791 - MMJJ_RS04625 0.48 +0.6 862,817 + MMJJ_RS04625 0.50 +0.0 862,832 - MMJJ_RS04625 0.51 +0.4 862,836 - MMJJ_RS04625 0.51 -0.5 862,836 - MMJJ_RS04625 0.51 +0.3 862,836 - MMJJ_RS04625 0.51 -0.4 862,837 + MMJJ_RS04625 0.51 +1.4 862,837 + MMJJ_RS04625 0.51 +0.2 862,838 - MMJJ_RS04625 0.51 +1.4 862,838 - MMJJ_RS04625 0.51 -0.6 862,839 + MMJJ_RS04625 0.51 +0.2 862,839 + MMJJ_RS04625 0.51 +1.0 862,908 - MMJJ_RS04625 0.57 +0.1 862,909 + MMJJ_RS04625 0.57 +0.2 862,966 - MMJJ_RS04625 0.61 +0.1 862,966 - MMJJ_RS04625 0.61 +0.3 863,016 - MMJJ_RS04625 0.65 -0.2 863,016 - MMJJ_RS04625 0.65 +0.1 863,016 - MMJJ_RS04625 0.65 +1.0 863,017 + MMJJ_RS04625 0.65 +0.6 863,177 - MMJJ_RS04625 0.77 -0.5 863,178 + MMJJ_RS04625 0.77 -0.0 863,182 + MMJJ_RS04625 0.77 -1.4 863,196 - MMJJ_RS04625 0.78 +0.6 863,271 - MMJJ_RS04625 0.84 +0.1 863,271 - MMJJ_RS04625 0.84 +1.1 863,368 + -0.2 863,369 - -0.2 863,370 + -0.2 863,397 - +0.2 863,438 - +0.2 863,449 + +0.2 863,470 + -0.5 863,470 + -0.3 863,474 - -0.2 863,474 - +0.2 863,475 + +0.1 863,475 + -0.0 863,505 + +0.3 863,568 - -0.0 863,583 - +0.9 863,585 - -0.0 863,585 - +0.8 863,585 - +0.3 863,585 - +0.4 863,586 + -0.4 863,586 + -0.0 863,586 + +1.0 863,591 - -0.2 863,592 + -0.5 863,615 + +0.5 863,615 + +0.1 863,617 - +0.0 863,617 - -0.5 863,618 + +0.8 863,669 - +0.0 863,669 - -0.6 863,677 - -0.4 863,677 - +0.6 863,678 + +0.3 863,700 - +0.0 863,704 - -1.6 863,705 + +0.3 863,731 + +0.4 863,736 - -0.1 863,736 - +0.4 863,738 - -0.2 863,750 - +0.6 863,754 - +0.5 863,754 - +0.4 863,755 + -0.6 863,755 + -1.1 863,758 + +0.3 863,761 + +0.2 863,778 - +0.6 863,792 + +0.3 863,804 - -0.2 863,804 - +0.9 863,805 + +0.6 863,911 - MMJJ_RS04630 0.28 +2.2 864,015 - +1.0 864,182 + MMJJ_RS04635 0.32 -0.0 864,182 + MMJJ_RS04635 0.32 +0.1 864,186 + MMJJ_RS04635 0.32 -0.1 864,186 + MMJJ_RS04635 0.32 +1.2 864,199 - MMJJ_RS04635 0.35 -0.6 864,208 - MMJJ_RS04635 0.37 +0.7 864,208 - MMJJ_RS04635 0.37 -0.0 864,209 + MMJJ_RS04635 0.37 -0.9 864,213 - MMJJ_RS04635 0.38 -0.3 864,216 - MMJJ_RS04635 0.38 +0.5 864,444 - MMJJ_RS04635 0.82 +0.5 864,486 - -0.4 864,486 - -1.5 864,486 - -0.1 864,487 + -0.9
Or see this region's nucleotide sequence