Experiment: 2-Furfuraldehyde 0.125 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt obgE and yhbE are separated by 15 nucleotides yhbE and rpmA are separated by 126 nucleotides rpmA and rplU are separated by 20 nucleotides rplU and ispB are separated by 258 nucleotides
b3183: obgE - GTPase involved in cell partioning and DNA repair (NCBI), at 3,328,604 to 3,329,776
obgE
b3184: yhbE - conserved inner membrane protein (NCBI), at 3,329,792 to 3,330,757
yhbE
b3185: rpmA - 50S ribosomal protein L27 (NCBI), at 3,330,884 to 3,331,141
rpmA
b3186: rplU - 50S ribosomal protein L21 (NCBI), at 3,331,162 to 3,331,473
rplU
b3187: ispB - octaprenyl diphosphate synthase (NCBI), at 3,331,732 to 3,332,703
ispB
Position (kb)
3329
3330
3331 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3328.805 kb on - strand, within obgE at 3328.813 kb on - strand, within obgE at 3329.785 kb on + strand at 3329.811 kb on + strand at 3329.871 kb on - strand at 3329.893 kb on - strand, within yhbE at 3329.893 kb on - strand, within yhbE at 3329.904 kb on + strand at 3329.910 kb on + strand, within yhbE at 3329.946 kb on + strand, within yhbE at 3330.050 kb on - strand, within yhbE at 3330.119 kb on - strand, within yhbE at 3330.119 kb on - strand, within yhbE at 3330.129 kb on + strand, within yhbE at 3330.130 kb on + strand, within yhbE at 3330.130 kb on + strand, within yhbE at 3330.137 kb on - strand, within yhbE at 3330.198 kb on - strand, within yhbE at 3330.232 kb on + strand, within yhbE at 3330.234 kb on + strand, within yhbE at 3330.240 kb on - strand, within yhbE at 3330.240 kb on - strand, within yhbE at 3330.240 kb on - strand, within yhbE at 3330.271 kb on - strand, within yhbE at 3330.272 kb on - strand, within yhbE at 3330.285 kb on + strand, within yhbE at 3330.292 kb on - strand, within yhbE at 3330.347 kb on + strand, within yhbE at 3330.355 kb on - strand, within yhbE at 3330.437 kb on - strand, within yhbE at 3330.451 kb on + strand, within yhbE at 3330.491 kb on + strand, within yhbE at 3330.547 kb on + strand, within yhbE at 3330.608 kb on - strand, within yhbE at 3330.610 kb on + strand, within yhbE at 3330.618 kb on - strand, within yhbE at 3330.618 kb on - strand, within yhbE at 3330.624 kb on + strand, within yhbE at 3330.672 kb on - strand at 3330.679 kb on + strand at 3330.733 kb on + strand at 3330.786 kb on + strand at 3330.791 kb on + strand at 3330.799 kb on - strand at 3330.844 kb on - strand at 3330.844 kb on - strand at 3331.575 kb on + strand at 3331.575 kb on + strand at 3331.609 kb on + strand at 3331.621 kb on + strand at 3331.703 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Furfuraldehyde 0.125 vol% remove 3,328,805 - obgE b3183 0.17 -0.4 3,328,813 - obgE b3183 0.18 -1.7 3,329,785 + -0.7 3,329,811 + +0.8 3,329,871 - +0.2 3,329,893 - yhbE b3184 0.10 +1.6 3,329,893 - yhbE b3184 0.10 -0.3 3,329,904 + +2.2 3,329,910 + yhbE b3184 0.12 -1.4 3,329,946 + yhbE b3184 0.16 -1.2 3,330,050 - yhbE b3184 0.27 -0.6 3,330,119 - yhbE b3184 0.34 -0.2 3,330,119 - yhbE b3184 0.34 -0.7 3,330,129 + yhbE b3184 0.35 -0.8 3,330,130 + yhbE b3184 0.35 -0.4 3,330,130 + yhbE b3184 0.35 +1.2 3,330,137 - yhbE b3184 0.36 +0.5 3,330,198 - yhbE b3184 0.42 -0.7 3,330,232 + yhbE b3184 0.46 -1.7 3,330,234 + yhbE b3184 0.46 -0.9 3,330,240 - yhbE b3184 0.46 -0.8 3,330,240 - yhbE b3184 0.46 +0.4 3,330,240 - yhbE b3184 0.46 -1.4 3,330,271 - yhbE b3184 0.50 +0.4 3,330,272 - yhbE b3184 0.50 +0.5 3,330,285 + yhbE b3184 0.51 -1.2 3,330,292 - yhbE b3184 0.52 -1.2 3,330,347 + yhbE b3184 0.57 -1.8 3,330,355 - yhbE b3184 0.58 +0.1 3,330,437 - yhbE b3184 0.67 -0.9 3,330,451 + yhbE b3184 0.68 -0.4 3,330,491 + yhbE b3184 0.72 -0.8 3,330,547 + yhbE b3184 0.78 -1.2 3,330,608 - yhbE b3184 0.84 -0.2 3,330,610 + yhbE b3184 0.85 -1.0 3,330,618 - yhbE b3184 0.86 -1.1 3,330,618 - yhbE b3184 0.86 -0.4 3,330,624 + yhbE b3184 0.86 -2.2 3,330,672 - +0.2 3,330,679 + -1.0 3,330,733 + -0.8 3,330,786 + -2.7 3,330,791 + -1.4 3,330,799 - +0.2 3,330,844 - +0.8 3,330,844 - -0.7 3,331,575 + +0.4 3,331,575 + +0.9 3,331,609 + -1.3 3,331,621 + -0.1 3,331,703 - +0.2
Or see this region's nucleotide sequence