Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0741 and Shew_0742 are separated by 141 nucleotides Shew_0742 and Shew_0743 are separated by 164 nucleotides Shew_0743 and Shew_0744 are separated by 103 nucleotides Shew_0744 and Shew_0745 are separated by 183 nucleotides Shew_0745 and Shew_0746 are separated by 25 nucleotides
Shew_0741: Shew_0741 - GCN5-related N-acetyltransferase (RefSeq), at 843,511 to 843,954
_0741
Shew_0742: Shew_0742 - hypothetical protein (RefSeq), at 844,096 to 844,401
_0742
Shew_0743: Shew_0743 - sodium:dicarboxylate symporter (RefSeq), at 844,566 to 845,867
_0743
Shew_0744: Shew_0744 - protein of unknown function DUF306, Meta and HslJ (RefSeq), at 845,971 to 846,384
_0744
Shew_0745: Shew_0745 - hypothetical protein (RefSeq), at 846,568 to 846,807
_0745
Shew_0746: Shew_0746 - diguanylate cyclase (RefSeq), at 846,833 to 847,777
_0746
Position (kb) 844
845
846 Strain fitness (log2 ratio) -2
-1
0
1
2 at 843.716 kb on + strand, within Shew_0741 at 843.846 kb on + strand, within Shew_0741 at 843.874 kb on - strand, within Shew_0741 at 843.998 kb on - strand at 844.056 kb on - strand at 844.432 kb on + strand at 844.447 kb on + strand at 845.145 kb on - strand, within Shew_0743 at 845.304 kb on + strand, within Shew_0743 at 845.312 kb on - strand, within Shew_0743 at 845.324 kb on + strand, within Shew_0743 at 845.324 kb on + strand, within Shew_0743 at 845.332 kb on - strand, within Shew_0743 at 845.332 kb on - strand, within Shew_0743 at 845.332 kb on - strand, within Shew_0743 at 845.332 kb on - strand, within Shew_0743 at 845.372 kb on + strand, within Shew_0743 at 845.518 kb on - strand, within Shew_0743 at 845.786 kb on + strand at 846.029 kb on + strand, within Shew_0744 at 846.254 kb on + strand, within Shew_0744 at 846.262 kb on - strand, within Shew_0744 at 846.262 kb on - strand, within Shew_0744 at 846.262 kb on - strand, within Shew_0744 at 846.262 kb on - strand, within Shew_0744 at 846.540 kb on + strand at 846.831 kb on + strand at 846.831 kb on + strand at 846.831 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 843,716 + Shew_0741 0.46 +0.9 843,846 + Shew_0741 0.75 -0.2 843,874 - Shew_0741 0.82 -1.3 843,998 - +0.6 844,056 - -0.3 844,432 + +0.8 844,447 + +0.1 845,145 - Shew_0743 0.44 +0.7 845,304 + Shew_0743 0.57 +0.2 845,312 - Shew_0743 0.57 +2.5 845,324 + Shew_0743 0.58 -1.3 845,324 + Shew_0743 0.58 -0.2 845,332 - Shew_0743 0.59 -0.3 845,332 - Shew_0743 0.59 -0.4 845,332 - Shew_0743 0.59 +0.1 845,332 - Shew_0743 0.59 -2.3 845,372 + Shew_0743 0.62 +1.0 845,518 - Shew_0743 0.73 +0.2 845,786 + +0.5 846,029 + Shew_0744 0.14 -0.0 846,254 + Shew_0744 0.68 -0.6 846,262 - Shew_0744 0.70 -2.3 846,262 - Shew_0744 0.70 +0.4 846,262 - Shew_0744 0.70 -0.7 846,262 - Shew_0744 0.70 -0.0 846,540 + -0.3 846,831 + +0.0 846,831 + +0.6 846,831 + +0.2
Or see this region's nucleotide sequence