Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0408 and Shew_0409 overlap by 77 nucleotides Shew_0409 and Shew_0410 are separated by 41 nucleotides Shew_0410 and Shew_0411 are separated by 192 nucleotides
Shew_0408: Shew_0408 - hypothetical protein (RefSeq), at 457,734 to 462,044
_0408
Shew_0409: Shew_0409 - Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase (RefSeq), at 461,968 to 462,798
_0409
Shew_0410: Shew_0410 - peptidase U62, modulator of DNA gyrase (RefSeq), at 462,840 to 464,288
_0410
Shew_0411: Shew_0411 - outer membrane efflux protein (RefSeq), at 464,481 to 465,884
_0411
Position (kb) 462
463
464
465 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 462.097 kb on - strand, within Shew_0409 at 462.391 kb on - strand, within Shew_0409 at 462.431 kb on + strand, within Shew_0409 at 462.446 kb on - strand, within Shew_0409 at 462.615 kb on + strand, within Shew_0409 at 462.717 kb on + strand at 463.192 kb on + strand, within Shew_0410 at 463.287 kb on - strand, within Shew_0410 at 463.869 kb on + strand, within Shew_0410 at 463.882 kb on - strand, within Shew_0410 at 463.940 kb on - strand, within Shew_0410 at 464.291 kb on - strand at 464.626 kb on - strand, within Shew_0411 at 464.665 kb on - strand, within Shew_0411 at 464.937 kb on + strand, within Shew_0411 at 465.036 kb on - strand, within Shew_0411 at 465.243 kb on - strand, within Shew_0411 at 465.268 kb on + strand, within Shew_0411 at 465.276 kb on - strand, within Shew_0411
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 462,097 - Shew_0409 0.16 -0.7 462,391 - Shew_0409 0.51 -0.2 462,431 + Shew_0409 0.56 -0.4 462,446 - Shew_0409 0.58 +0.0 462,615 + Shew_0409 0.78 +0.3 462,717 + +0.2 463,192 + Shew_0410 0.24 +1.6 463,287 - Shew_0410 0.31 -1.7 463,869 + Shew_0410 0.71 +0.5 463,882 - Shew_0410 0.72 +0.6 463,940 - Shew_0410 0.76 +1.1 464,291 - +0.3 464,626 - Shew_0411 0.10 +0.1 464,665 - Shew_0411 0.13 -1.7 464,937 + Shew_0411 0.32 -2.4 465,036 - Shew_0411 0.40 -3.0 465,243 - Shew_0411 0.54 -0.8 465,268 + Shew_0411 0.56 -1.1 465,276 - Shew_0411 0.57 -1.1
Or see this region's nucleotide sequence