Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04345 and MMJJ_RS04350 overlap by 8 nucleotides MMJJ_RS04350 and MMJJ_RS09405 are separated by 18 nucleotides MMJJ_RS09405 and MMJJ_RS04360 are separated by 206 nucleotides MMJJ_RS04360 and MMJJ_RS04365 are separated by 690 nucleotides
MMJJ_RS04345: MMJJ_RS04345 - DUF2080 family transposase-associated protein, at 811,442 to 811,666
_RS04345
MMJJ_RS04350: MMJJ_RS04350 - hypothetical protein, at 811,659 to 812,162
_RS04350
MMJJ_RS09405: MMJJ_RS09405 - hypothetical protein, at 812,181 to 812,360
_RS09405
MMJJ_RS04360: MMJJ_RS04360 - winged helix-turn-helix transcriptional regulator, at 812,567 to 812,869
_RS04360
MMJJ_RS04365: MMJJ_RS04365 - helix-turn-helix transcriptional regulator, at 813,560 to 813,838
_RS04365
Position (kb) 812
813 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 811.571 kb on - strand, within MMJJ_RS04345 at 811.582 kb on - strand, within MMJJ_RS04345 at 811.582 kb on - strand, within MMJJ_RS04345 at 811.583 kb on + strand, within MMJJ_RS04345 at 811.583 kb on + strand, within MMJJ_RS04345 at 811.635 kb on + strand, within MMJJ_RS04345 at 811.655 kb on - strand at 811.656 kb on + strand at 811.788 kb on - strand, within MMJJ_RS04350 at 811.789 kb on + strand, within MMJJ_RS04350 at 811.789 kb on + strand, within MMJJ_RS04350 at 811.932 kb on + strand, within MMJJ_RS04350 at 811.932 kb on + strand, within MMJJ_RS04350 at 811.965 kb on - strand, within MMJJ_RS04350 at 811.966 kb on + strand, within MMJJ_RS04350 at 811.966 kb on + strand, within MMJJ_RS04350 at 812.059 kb on - strand, within MMJJ_RS04350 at 812.076 kb on - strand, within MMJJ_RS04350 at 812.076 kb on - strand, within MMJJ_RS04350 at 812.089 kb on + strand, within MMJJ_RS04350 at 812.124 kb on + strand at 812.127 kb on - strand at 812.127 kb on - strand at 812.128 kb on + strand at 812.163 kb on + strand at 812.180 kb on + strand at 812.183 kb on + strand at 812.203 kb on + strand, within MMJJ_RS09405 at 812.221 kb on + strand, within MMJJ_RS09405 at 812.279 kb on - strand, within MMJJ_RS09405 at 812.280 kb on + strand, within MMJJ_RS09405 at 812.313 kb on - strand, within MMJJ_RS09405 at 812.314 kb on + strand, within MMJJ_RS09405 at 812.314 kb on + strand, within MMJJ_RS09405 at 812.354 kb on + strand at 812.446 kb on + strand at 812.450 kb on - strand at 812.450 kb on - strand at 812.451 kb on + strand at 812.451 kb on + strand at 812.451 kb on + strand at 812.455 kb on + strand at 812.455 kb on + strand at 812.459 kb on - strand at 812.459 kb on - strand at 812.460 kb on + strand at 812.514 kb on - strand at 812.519 kb on + strand at 812.519 kb on + strand at 812.523 kb on + strand at 812.549 kb on - strand at 812.563 kb on - strand at 812.563 kb on - strand at 812.567 kb on + strand at 812.567 kb on + strand at 812.567 kb on + strand at 812.637 kb on - strand, within MMJJ_RS04360 at 812.657 kb on + strand, within MMJJ_RS04360 at 812.660 kb on - strand, within MMJJ_RS04360 at 812.660 kb on - strand, within MMJJ_RS04360 at 812.722 kb on + strand, within MMJJ_RS04360 at 812.768 kb on - strand, within MMJJ_RS04360 at 812.772 kb on + strand, within MMJJ_RS04360 at 812.773 kb on - strand, within MMJJ_RS04360 at 812.773 kb on - strand, within MMJJ_RS04360 at 812.773 kb on - strand, within MMJJ_RS04360 at 812.774 kb on + strand, within MMJJ_RS04360 at 812.774 kb on + strand, within MMJJ_RS04360 at 812.774 kb on + strand, within MMJJ_RS04360 at 812.836 kb on - strand, within MMJJ_RS04360 at 812.836 kb on - strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.837 kb on + strand, within MMJJ_RS04360 at 812.840 kb on - strand at 812.841 kb on + strand at 812.841 kb on + strand at 812.857 kb on - strand at 812.889 kb on + strand at 812.895 kb on - strand at 812.917 kb on - strand at 812.917 kb on - strand at 813.158 kb on + strand at 813.158 kb on + strand at 813.158 kb on + strand at 813.158 kb on + strand at 813.162 kb on + strand at 813.169 kb on + strand at 813.169 kb on + strand at 813.216 kb on - strand at 813.216 kb on - strand at 813.217 kb on + strand at 813.219 kb on - strand at 813.235 kb on + strand at 813.269 kb on - strand at 813.270 kb on + strand at 813.270 kb on + strand at 813.293 kb on + strand at 813.306 kb on - strand at 813.306 kb on - strand at 813.307 kb on + strand at 813.311 kb on + strand at 813.330 kb on + strand at 813.345 kb on - strand at 813.346 kb on + strand at 813.346 kb on + strand at 813.346 kb on + strand at 813.346 kb on + strand at 813.346 kb on + strand at 813.353 kb on + strand at 813.354 kb on - strand at 813.354 kb on - strand at 813.355 kb on + strand at 813.355 kb on + strand at 813.366 kb on + strand at 813.379 kb on - strand at 813.413 kb on + strand at 813.416 kb on - strand at 813.432 kb on - strand at 813.448 kb on + strand at 813.448 kb on + strand at 813.457 kb on - strand at 813.464 kb on - strand at 813.465 kb on + strand at 813.566 kb on + strand at 813.568 kb on - strand at 813.588 kb on - strand, within MMJJ_RS04365 at 813.591 kb on - strand, within MMJJ_RS04365 at 813.591 kb on - strand, within MMJJ_RS04365 at 813.592 kb on + strand, within MMJJ_RS04365 at 813.593 kb on - strand, within MMJJ_RS04365 at 813.593 kb on - strand, within MMJJ_RS04365 at 813.593 kb on - strand, within MMJJ_RS04365 at 813.598 kb on + strand, within MMJJ_RS04365 at 813.598 kb on + strand, within MMJJ_RS04365 at 813.633 kb on - strand, within MMJJ_RS04365 at 813.637 kb on - strand, within MMJJ_RS04365 at 813.637 kb on - strand, within MMJJ_RS04365 at 813.638 kb on + strand, within MMJJ_RS04365 at 813.641 kb on + strand, within MMJJ_RS04365 at 813.667 kb on - strand, within MMJJ_RS04365 at 813.690 kb on - strand, within MMJJ_RS04365 at 813.691 kb on + strand, within MMJJ_RS04365 at 813.701 kb on + strand, within MMJJ_RS04365 at 813.821 kb on - strand at 813.822 kb on + strand at 813.826 kb on - strand at 813.826 kb on - strand at 813.827 kb on + strand at 813.827 kb on + strand at 813.827 kb on + strand at 813.827 kb on + strand at 813.827 kb on + strand at 813.827 kb on + strand at 813.827 kb on + strand at 813.827 kb on + strand at 813.837 kb on + strand at 813.839 kb on - strand at 813.839 kb on - strand at 813.840 kb on + strand at 813.840 kb on + strand at 813.840 kb on + strand at 813.845 kb on + strand at 813.847 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 811,571 - MMJJ_RS04345 0.57 +0.3 811,582 - MMJJ_RS04345 0.62 -1.4 811,582 - MMJJ_RS04345 0.62 -0.7 811,583 + MMJJ_RS04345 0.63 -0.3 811,583 + MMJJ_RS04345 0.63 +2.2 811,635 + MMJJ_RS04345 0.86 -1.1 811,655 - -0.8 811,656 + +1.6 811,788 - MMJJ_RS04350 0.26 -0.9 811,789 + MMJJ_RS04350 0.26 +0.4 811,789 + MMJJ_RS04350 0.26 +0.3 811,932 + MMJJ_RS04350 0.54 -1.2 811,932 + MMJJ_RS04350 0.54 -0.3 811,965 - MMJJ_RS04350 0.61 -1.3 811,966 + MMJJ_RS04350 0.61 +1.7 811,966 + MMJJ_RS04350 0.61 -0.5 812,059 - MMJJ_RS04350 0.79 +0.3 812,076 - MMJJ_RS04350 0.83 -2.2 812,076 - MMJJ_RS04350 0.83 +0.3 812,089 + MMJJ_RS04350 0.85 -0.8 812,124 + -0.7 812,127 - -0.2 812,127 - -0.5 812,128 + -0.0 812,163 + -0.3 812,180 + -0.3 812,183 + +1.0 812,203 + MMJJ_RS09405 0.12 -0.4 812,221 + MMJJ_RS09405 0.22 +0.3 812,279 - MMJJ_RS09405 0.54 +0.5 812,280 + MMJJ_RS09405 0.55 +0.3 812,313 - MMJJ_RS09405 0.73 -1.2 812,314 + MMJJ_RS09405 0.74 +0.5 812,314 + MMJJ_RS09405 0.74 +0.1 812,354 + +2.3 812,446 + +2.1 812,450 - +0.2 812,450 - +0.4 812,451 + +0.1 812,451 + -0.1 812,451 + -0.1 812,455 + -0.0 812,455 + +0.4 812,459 - +0.3 812,459 - -0.1 812,460 + -0.1 812,514 - -3.5 812,519 + -1.8 812,519 + -1.2 812,523 + -3.7 812,549 - -1.7 812,563 - -3.6 812,563 - -4.8 812,567 + -3.9 812,567 + -4.5 812,567 + -4.5 812,637 - MMJJ_RS04360 0.23 -3.1 812,657 + MMJJ_RS04360 0.30 -3.2 812,660 - MMJJ_RS04360 0.31 -4.0 812,660 - MMJJ_RS04360 0.31 -2.8 812,722 + MMJJ_RS04360 0.51 -4.0 812,768 - MMJJ_RS04360 0.66 -3.5 812,772 + MMJJ_RS04360 0.68 -1.7 812,773 - MMJJ_RS04360 0.68 -3.2 812,773 - MMJJ_RS04360 0.68 -3.6 812,773 - MMJJ_RS04360 0.68 -2.9 812,774 + MMJJ_RS04360 0.68 -3.7 812,774 + MMJJ_RS04360 0.68 -3.8 812,774 + MMJJ_RS04360 0.68 -5.0 812,836 - MMJJ_RS04360 0.89 +0.3 812,836 - MMJJ_RS04360 0.89 +0.3 812,837 + MMJJ_RS04360 0.89 +2.2 812,837 + MMJJ_RS04360 0.89 +0.2 812,837 + MMJJ_RS04360 0.89 +0.9 812,837 + MMJJ_RS04360 0.89 +0.1 812,837 + MMJJ_RS04360 0.89 -0.5 812,837 + MMJJ_RS04360 0.89 +1.8 812,837 + MMJJ_RS04360 0.89 -0.6 812,837 + MMJJ_RS04360 0.89 +1.3 812,837 + MMJJ_RS04360 0.89 +0.2 812,837 + MMJJ_RS04360 0.89 -3.5 812,837 + MMJJ_RS04360 0.89 +1.2 812,840 - +0.2 812,841 + -0.8 812,841 + -0.2 812,857 - -0.1 812,889 + +0.2 812,895 - -0.3 812,917 - +0.1 812,917 - -1.0 813,158 + -0.8 813,158 + -0.2 813,158 + -0.2 813,158 + -0.1 813,162 + +1.9 813,169 + -0.6 813,169 + +0.2 813,216 - -0.5 813,216 - +0.5 813,217 + +1.2 813,219 - -0.8 813,235 + -0.8 813,269 - -0.5 813,270 + -0.0 813,270 + -1.6 813,293 + -0.1 813,306 - +0.3 813,306 - +0.5 813,307 + -0.6 813,311 + +0.6 813,330 + -0.6 813,345 - -1.0 813,346 + +2.1 813,346 + +0.3 813,346 + -0.0 813,346 + +0.1 813,346 + -0.5 813,353 + -1.6 813,354 - -1.0 813,354 - -0.2 813,355 + -0.8 813,355 + -1.1 813,366 + -1.2 813,379 - -0.3 813,413 + +0.2 813,416 - -0.8 813,432 - +0.2 813,448 + -0.1 813,448 + +1.7 813,457 - +0.1 813,464 - -0.6 813,465 + +0.6 813,566 + -1.1 813,568 - +0.1 813,588 - MMJJ_RS04365 0.10 +0.2 813,591 - MMJJ_RS04365 0.11 +0.6 813,591 - MMJJ_RS04365 0.11 +0.4 813,592 + MMJJ_RS04365 0.11 +0.9 813,593 - MMJJ_RS04365 0.12 -1.5 813,593 - MMJJ_RS04365 0.12 -0.7 813,593 - MMJJ_RS04365 0.12 +0.2 813,598 + MMJJ_RS04365 0.14 -0.9 813,598 + MMJJ_RS04365 0.14 +0.5 813,633 - MMJJ_RS04365 0.26 -0.2 813,637 - MMJJ_RS04365 0.28 +0.2 813,637 - MMJJ_RS04365 0.28 +0.3 813,638 + MMJJ_RS04365 0.28 +2.5 813,641 + MMJJ_RS04365 0.29 -0.5 813,667 - MMJJ_RS04365 0.38 +1.2 813,690 - MMJJ_RS04365 0.47 +0.7 813,691 + MMJJ_RS04365 0.47 -0.6 813,701 + MMJJ_RS04365 0.51 -0.2 813,821 - -0.0 813,822 + -0.7 813,826 - -0.4 813,826 - -0.3 813,827 + +1.2 813,827 + +0.1 813,827 + +1.2 813,827 + +0.2 813,827 + -0.4 813,827 + +2.1 813,827 + +0.4 813,827 + -1.5 813,837 + +1.0 813,839 - +1.2 813,839 - -0.9 813,840 + -0.2 813,840 + -0.9 813,840 + +1.9 813,845 + +1.1 813,847 - -0.5
Or see this region's nucleotide sequence