Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4494 and Pf6N2E2_4495 are separated by 24 nucleotides Pf6N2E2_4495 and Pf6N2E2_4496 are separated by 14 nucleotides Pf6N2E2_4496 and Pf6N2E2_4497 are separated by 132 nucleotides
Pf6N2E2_4494 - Adenylate cyclase (EC 4.6.1.1), at 5,226,306 to 5,227,673
_4494
Pf6N2E2_4495 - hypothetical protein, at 5,227,698 to 5,227,817
_4495
Pf6N2E2_4496 - Acetylornithine deacetylase (EC 3.5.1.16), at 5,227,832 to 5,228,989
_4496
Pf6N2E2_4497 - N-acetylglutamate synthase (EC 2.3.1.1), at 5,229,122 to 5,230,420
_4497
Position (kb)
5227
5228
5229 Strain fitness (log2 ratio)
-1
0
1
2 at 5227.049 kb on - strand, within Pf6N2E2_4494 at 5227.049 kb on - strand, within Pf6N2E2_4494 at 5227.064 kb on - strand, within Pf6N2E2_4494 at 5227.417 kb on + strand, within Pf6N2E2_4494 at 5227.417 kb on + strand, within Pf6N2E2_4494 at 5227.418 kb on - strand, within Pf6N2E2_4494 at 5227.549 kb on + strand at 5227.549 kb on + strand at 5227.550 kb on - strand at 5227.550 kb on - strand at 5227.739 kb on + strand, within Pf6N2E2_4495 at 5227.739 kb on + strand, within Pf6N2E2_4495 at 5227.739 kb on + strand, within Pf6N2E2_4495 at 5227.739 kb on + strand, within Pf6N2E2_4495 at 5227.739 kb on + strand, within Pf6N2E2_4495 at 5227.758 kb on - strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.815 kb on + strand at 5227.815 kb on + strand at 5227.815 kb on + strand at 5227.816 kb on - strand at 5227.816 kb on - strand at 5228.046 kb on + strand, within Pf6N2E2_4496 at 5228.686 kb on + strand, within Pf6N2E2_4496 at 5228.686 kb on + strand, within Pf6N2E2_4496 at 5228.702 kb on + strand, within Pf6N2E2_4496 at 5228.702 kb on + strand, within Pf6N2E2_4496 at 5228.825 kb on + strand, within Pf6N2E2_4496 at 5228.825 kb on + strand, within Pf6N2E2_4496 at 5228.825 kb on + strand, within Pf6N2E2_4496 at 5228.825 kb on + strand, within Pf6N2E2_4496 at 5228.825 kb on + strand, within Pf6N2E2_4496 at 5228.826 kb on - strand, within Pf6N2E2_4496 at 5228.826 kb on - strand, within Pf6N2E2_4496 at 5228.900 kb on + strand at 5228.901 kb on - strand at 5228.901 kb on - strand at 5228.960 kb on + strand at 5229.100 kb on + strand at 5229.100 kb on + strand at 5229.100 kb on + strand at 5229.104 kb on + strand at 5229.107 kb on + strand at 5229.107 kb on + strand at 5229.107 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 5,227,049 - Pf6N2E2_4494 0.54 -0.0 5,227,049 - Pf6N2E2_4494 0.54 +0.2 5,227,064 - Pf6N2E2_4494 0.55 -1.2 5,227,417 + Pf6N2E2_4494 0.81 -0.9 5,227,417 + Pf6N2E2_4494 0.81 +0.1 5,227,418 - Pf6N2E2_4494 0.81 +1.1 5,227,549 + +0.9 5,227,549 + +0.6 5,227,550 - -0.7 5,227,550 - +0.1 5,227,739 + Pf6N2E2_4495 0.34 +1.6 5,227,739 + Pf6N2E2_4495 0.34 +0.2 5,227,739 + Pf6N2E2_4495 0.34 -1.4 5,227,739 + Pf6N2E2_4495 0.34 -0.2 5,227,739 + Pf6N2E2_4495 0.34 +0.6 5,227,758 - Pf6N2E2_4495 0.50 +0.0 5,227,796 + Pf6N2E2_4495 0.82 +1.0 5,227,796 + Pf6N2E2_4495 0.82 +0.3 5,227,796 + Pf6N2E2_4495 0.82 +1.2 5,227,796 + Pf6N2E2_4495 0.82 +0.8 5,227,796 + Pf6N2E2_4495 0.82 +0.2 5,227,796 + Pf6N2E2_4495 0.82 -0.8 5,227,796 + Pf6N2E2_4495 0.82 +1.3 5,227,796 + Pf6N2E2_4495 0.82 -1.0 5,227,796 + Pf6N2E2_4495 0.82 +1.3 5,227,796 + -0.5 5,227,797 - Pf6N2E2_4495 0.82 +0.5 5,227,797 - Pf6N2E2_4495 0.82 +0.2 5,227,797 - Pf6N2E2_4495 0.82 -0.3 5,227,797 - Pf6N2E2_4495 0.82 +0.8 5,227,797 - Pf6N2E2_4495 0.82 -0.4 5,227,797 - Pf6N2E2_4495 0.82 +1.1 5,227,797 - Pf6N2E2_4495 0.82 +0.4 5,227,815 + +1.6 5,227,815 + -1.0 5,227,815 + -0.5 5,227,816 - -0.1 5,227,816 - +0.6 5,228,046 + Pf6N2E2_4496 0.18 +0.3 5,228,686 + Pf6N2E2_4496 0.74 +1.8 5,228,686 + Pf6N2E2_4496 0.74 +1.8 5,228,702 + Pf6N2E2_4496 0.75 +0.9 5,228,702 + Pf6N2E2_4496 0.75 +0.4 5,228,825 + Pf6N2E2_4496 0.86 +0.5 5,228,825 + Pf6N2E2_4496 0.86 +0.6 5,228,825 + Pf6N2E2_4496 0.86 +0.0 5,228,825 + Pf6N2E2_4496 0.86 +1.2 5,228,825 + Pf6N2E2_4496 0.86 +1.5 5,228,826 - Pf6N2E2_4496 0.86 +0.5 5,228,826 - Pf6N2E2_4496 0.86 -0.3 5,228,900 + +0.7 5,228,901 - +0.8 5,228,901 - -0.1 5,228,960 + +0.1 5,229,100 + -0.3 5,229,100 + -0.4 5,229,100 + +0.7 5,229,104 + -0.3 5,229,107 + +0.8 5,229,107 + -0.4 5,229,107 + +1.7
Or see this region's nucleotide sequence