Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3612 and Pf6N2E2_3613 are separated by 85 nucleotides Pf6N2E2_3613 and Pf6N2E2_3614 are separated by 12 nucleotides Pf6N2E2_3614 and Pf6N2E2_3615 are separated by 91 nucleotides
Pf6N2E2_3612 - HflK protein, at 4,206,586 to 4,207,758
_3612
Pf6N2E2_3613 - GTP-binding protein HflX, at 4,207,844 to 4,209,145
_3613
Pf6N2E2_3614 - RNA-binding protein Hfq, at 4,209,158 to 4,209,418
_3614
Pf6N2E2_3615 - tRNA dimethylallyltransferase (EC 2.5.1.75), at 4,209,510 to 4,210,481
_3615
Position (kb)
4207
4208
4209
4210 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4206.981 kb on - strand, within Pf6N2E2_3612 at 4207.394 kb on + strand, within Pf6N2E2_3612 at 4207.394 kb on + strand, within Pf6N2E2_3612 at 4207.394 kb on + strand, within Pf6N2E2_3612 at 4207.395 kb on - strand, within Pf6N2E2_3612 at 4207.395 kb on - strand, within Pf6N2E2_3612 at 4207.449 kb on - strand, within Pf6N2E2_3612 at 4207.813 kb on - strand at 4207.813 kb on - strand at 4207.813 kb on - strand at 4207.813 kb on - strand at 4207.813 kb on - strand at 4207.846 kb on - strand at 4208.196 kb on + strand, within Pf6N2E2_3613 at 4208.197 kb on - strand, within Pf6N2E2_3613 at 4208.197 kb on - strand, within Pf6N2E2_3613 at 4208.197 kb on - strand, within Pf6N2E2_3613 at 4208.197 kb on - strand, within Pf6N2E2_3613 at 4208.197 kb on - strand, within Pf6N2E2_3613 at 4208.197 kb on - strand, within Pf6N2E2_3613 at 4208.197 kb on - strand, within Pf6N2E2_3613 at 4208.197 kb on - strand, within Pf6N2E2_3613 at 4208.197 kb on - strand, within Pf6N2E2_3613 at 4208.197 kb on - strand, within Pf6N2E2_3613 at 4208.197 kb on - strand, within Pf6N2E2_3613 at 4208.197 kb on - strand, within Pf6N2E2_3613 at 4208.199 kb on - strand, within Pf6N2E2_3613 at 4208.199 kb on - strand, within Pf6N2E2_3613 at 4208.336 kb on - strand, within Pf6N2E2_3613 at 4208.359 kb on + strand, within Pf6N2E2_3613 at 4208.475 kb on + strand, within Pf6N2E2_3613 at 4208.476 kb on - strand, within Pf6N2E2_3613 at 4208.476 kb on - strand, within Pf6N2E2_3613 at 4208.476 kb on - strand, within Pf6N2E2_3613 at 4208.476 kb on - strand, within Pf6N2E2_3613 at 4208.476 kb on - strand, within Pf6N2E2_3613 at 4208.476 kb on - strand, within Pf6N2E2_3613 at 4208.497 kb on + strand, within Pf6N2E2_3613 at 4208.816 kb on - strand, within Pf6N2E2_3613 at 4208.834 kb on - strand, within Pf6N2E2_3613 at 4209.157 kb on - strand at 4209.157 kb on - strand at 4209.434 kb on - strand at 4209.444 kb on - strand at 4209.491 kb on - strand at 4209.520 kb on - strand at 4209.539 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 4,206,981 - Pf6N2E2_3612 0.34 -2.6 4,207,394 + Pf6N2E2_3612 0.69 +0.1 4,207,394 + Pf6N2E2_3612 0.69 -0.0 4,207,394 + Pf6N2E2_3612 0.69 +0.5 4,207,395 - Pf6N2E2_3612 0.69 +0.4 4,207,395 - Pf6N2E2_3612 0.69 +0.7 4,207,449 - Pf6N2E2_3612 0.74 -2.1 4,207,813 - -1.9 4,207,813 - -3.0 4,207,813 - -1.6 4,207,813 - -1.2 4,207,813 - -0.4 4,207,846 - -2.2 4,208,196 + Pf6N2E2_3613 0.27 -1.1 4,208,197 - Pf6N2E2_3613 0.27 +0.1 4,208,197 - Pf6N2E2_3613 0.27 +0.3 4,208,197 - Pf6N2E2_3613 0.27 -1.3 4,208,197 - Pf6N2E2_3613 0.27 -0.4 4,208,197 - Pf6N2E2_3613 0.27 -1.9 4,208,197 - Pf6N2E2_3613 0.27 -0.8 4,208,197 - Pf6N2E2_3613 0.27 -2.2 4,208,197 - Pf6N2E2_3613 0.27 -1.3 4,208,197 - Pf6N2E2_3613 0.27 -0.1 4,208,197 - Pf6N2E2_3613 0.27 -3.0 4,208,197 - Pf6N2E2_3613 0.27 -0.8 4,208,197 - Pf6N2E2_3613 0.27 +0.1 4,208,199 - Pf6N2E2_3613 0.27 -1.1 4,208,199 - Pf6N2E2_3613 0.27 +0.0 4,208,336 - Pf6N2E2_3613 0.38 -1.8 4,208,359 + Pf6N2E2_3613 0.40 -2.9 4,208,475 + Pf6N2E2_3613 0.48 -1.4 4,208,476 - Pf6N2E2_3613 0.49 -2.0 4,208,476 - Pf6N2E2_3613 0.49 -4.6 4,208,476 - Pf6N2E2_3613 0.49 -0.9 4,208,476 - Pf6N2E2_3613 0.49 +0.4 4,208,476 - Pf6N2E2_3613 0.49 -3.2 4,208,476 - Pf6N2E2_3613 0.49 -3.8 4,208,497 + Pf6N2E2_3613 0.50 +0.4 4,208,816 - Pf6N2E2_3613 0.75 -3.1 4,208,834 - Pf6N2E2_3613 0.76 -1.8 4,209,157 - -1.9 4,209,157 - -0.4 4,209,434 - -2.2 4,209,444 - -3.5 4,209,491 - +1.7 4,209,520 - +3.5 4,209,539 - +0.8
Or see this region's nucleotide sequence