Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4926 and Pf1N1B4_4927 overlap by 4 nucleotides Pf1N1B4_4927 and Pf1N1B4_4928 overlap by 19 nucleotides Pf1N1B4_4928 and Pf1N1B4_4929 overlap by 4 nucleotides
Pf1N1B4_4926 - ATPase components of various ABC-type transport systems, contain duplicated ATPase, at 5,459,110 to 5,460,951
_4926
Pf1N1B4_4927 - Hypothetical, similar to sarcosine oxidase alpha subunit, 2Fe-2S domain, at 5,460,948 to 5,461,256
_4927
Pf1N1B4_4928 - Ferredoxin reductase, at 5,461,238 to 5,462,629
_4928
Pf1N1B4_4929 - Opine oxidase subunit B, at 5,462,626 to 5,463,795
_4929
Position (kb)
5461
5462
5463 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5460.280 kb on + strand, within Pf1N1B4_4926 at 5460.280 kb on + strand, within Pf1N1B4_4926 at 5460.280 kb on + strand, within Pf1N1B4_4926 at 5460.281 kb on - strand, within Pf1N1B4_4926 at 5460.281 kb on - strand, within Pf1N1B4_4926 at 5460.281 kb on - strand, within Pf1N1B4_4926 at 5460.326 kb on + strand at 5460.327 kb on - strand, within Pf1N1B4_4926 at 5460.327 kb on - strand, within Pf1N1B4_4926 at 5460.538 kb on + strand, within Pf1N1B4_4926 at 5460.601 kb on + strand at 5460.665 kb on - strand, within Pf1N1B4_4926 at 5460.665 kb on - strand, within Pf1N1B4_4926 at 5460.665 kb on - strand, within Pf1N1B4_4926 at 5461.379 kb on + strand, within Pf1N1B4_4928 at 5461.402 kb on + strand, within Pf1N1B4_4928 at 5461.412 kb on + strand at 5461.425 kb on - strand, within Pf1N1B4_4928 at 5461.649 kb on + strand, within Pf1N1B4_4928 at 5461.649 kb on + strand, within Pf1N1B4_4928 at 5461.649 kb on + strand, within Pf1N1B4_4928 at 5461.649 kb on + strand, within Pf1N1B4_4928 at 5461.650 kb on - strand, within Pf1N1B4_4928 at 5461.650 kb on - strand at 5461.739 kb on + strand, within Pf1N1B4_4928 at 5461.740 kb on - strand, within Pf1N1B4_4928 at 5461.757 kb on + strand, within Pf1N1B4_4928 at 5461.935 kb on + strand, within Pf1N1B4_4928 at 5462.077 kb on + strand, within Pf1N1B4_4928 at 5462.154 kb on - strand at 5462.154 kb on - strand, within Pf1N1B4_4928 at 5462.634 kb on - strand at 5463.325 kb on + strand, within Pf1N1B4_4929 at 5463.353 kb on - strand, within Pf1N1B4_4929 at 5463.353 kb on - strand, within Pf1N1B4_4929 at 5463.379 kb on + strand, within Pf1N1B4_4929 at 5463.379 kb on + strand, within Pf1N1B4_4929 at 5463.460 kb on + strand at 5463.566 kb on - strand, within Pf1N1B4_4929 at 5463.619 kb on + strand, within Pf1N1B4_4929 at 5463.620 kb on - strand, within Pf1N1B4_4929 at 5463.623 kb on - strand, within Pf1N1B4_4929
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,460,280 + Pf1N1B4_4926 0.64 +0.0 5,460,280 + Pf1N1B4_4926 0.64 -0.7 5,460,280 + Pf1N1B4_4926 0.64 +0.4 5,460,281 - Pf1N1B4_4926 0.64 -1.6 5,460,281 - Pf1N1B4_4926 0.64 -0.5 5,460,281 - Pf1N1B4_4926 0.64 +0.5 5,460,326 + +1.7 5,460,327 - Pf1N1B4_4926 0.66 +0.5 5,460,327 - Pf1N1B4_4926 0.66 +1.7 5,460,538 + Pf1N1B4_4926 0.78 +0.3 5,460,601 + +2.0 5,460,665 - Pf1N1B4_4926 0.84 +0.4 5,460,665 - Pf1N1B4_4926 0.84 -1.6 5,460,665 - Pf1N1B4_4926 0.84 +0.3 5,461,379 + Pf1N1B4_4928 0.10 +0.9 5,461,402 + Pf1N1B4_4928 0.12 +1.5 5,461,412 + -2.3 5,461,425 - Pf1N1B4_4928 0.13 +0.7 5,461,649 + Pf1N1B4_4928 0.30 -1.1 5,461,649 + Pf1N1B4_4928 0.30 +1.7 5,461,649 + Pf1N1B4_4928 0.30 +1.3 5,461,649 + Pf1N1B4_4928 0.30 +1.3 5,461,650 - Pf1N1B4_4928 0.30 +1.0 5,461,650 - -0.3 5,461,739 + Pf1N1B4_4928 0.36 +0.7 5,461,740 - Pf1N1B4_4928 0.36 +1.0 5,461,757 + Pf1N1B4_4928 0.37 +0.5 5,461,935 + Pf1N1B4_4928 0.50 -0.8 5,462,077 + Pf1N1B4_4928 0.60 +0.3 5,462,154 - +0.7 5,462,154 - Pf1N1B4_4928 0.66 +0.3 5,462,634 - -0.2 5,463,325 + Pf1N1B4_4929 0.60 +0.3 5,463,353 - Pf1N1B4_4929 0.62 +0.4 5,463,353 - Pf1N1B4_4929 0.62 -1.3 5,463,379 + Pf1N1B4_4929 0.64 +0.3 5,463,379 + Pf1N1B4_4929 0.64 +0.9 5,463,460 + -0.3 5,463,566 - Pf1N1B4_4929 0.80 -0.3 5,463,619 + Pf1N1B4_4929 0.85 +0.3 5,463,620 - Pf1N1B4_4929 0.85 -0.3 5,463,623 - Pf1N1B4_4929 0.85 -1.9
Or see this region's nucleotide sequence