Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_439 and Pf1N1B4_440 are separated by 676 nucleotides
Pf1N1B4_439 - hypothetical protein, at 521,273 to 521,464
_439
Pf1N1B4_440 - hypothetical protein, at 522,141 to 522,383
_440
Position (kb)
521
522 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 520.288 kb on - strand at 520.382 kb on - strand at 520.795 kb on + strand at 520.904 kb on + strand at 520.956 kb on + strand at 521.017 kb on - strand at 521.049 kb on + strand at 521.049 kb on + strand at 521.050 kb on - strand at 521.050 kb on - strand at 521.050 kb on - strand at 521.050 kb on - strand at 521.145 kb on + strand at 521.145 kb on + strand at 521.145 kb on + strand at 521.148 kb on + strand at 521.148 kb on + strand at 521.149 kb on - strand at 521.149 kb on - strand at 521.181 kb on + strand at 521.182 kb on - strand at 521.202 kb on + strand at 521.202 kb on + strand at 521.203 kb on - strand at 521.203 kb on - strand at 521.203 kb on - strand at 521.273 kb on - strand at 521.357 kb on + strand, within Pf1N1B4_439 at 521.357 kb on + strand, within Pf1N1B4_439 at 521.357 kb on + strand, within Pf1N1B4_439 at 521.357 kb on + strand, within Pf1N1B4_439 at 521.358 kb on - strand, within Pf1N1B4_439 at 521.358 kb on - strand, within Pf1N1B4_439 at 521.358 kb on - strand, within Pf1N1B4_439 at 521.358 kb on - strand, within Pf1N1B4_439 at 521.358 kb on - strand, within Pf1N1B4_439 at 521.363 kb on + strand, within Pf1N1B4_439 at 521.363 kb on + strand, within Pf1N1B4_439 at 521.363 kb on + strand, within Pf1N1B4_439 at 521.364 kb on - strand, within Pf1N1B4_439 at 521.364 kb on - strand, within Pf1N1B4_439 at 521.403 kb on - strand, within Pf1N1B4_439 at 521.403 kb on - strand, within Pf1N1B4_439 at 521.406 kb on - strand, within Pf1N1B4_439 at 521.444 kb on + strand, within Pf1N1B4_439 at 521.444 kb on + strand, within Pf1N1B4_439 at 521.444 kb on + strand, within Pf1N1B4_439 at 521.444 kb on + strand, within Pf1N1B4_439 at 521.445 kb on - strand at 521.463 kb on - strand at 521.463 kb on - strand at 521.533 kb on + strand at 521.534 kb on - strand at 521.534 kb on - strand at 521.571 kb on + strand at 521.604 kb on + strand at 521.604 kb on + strand at 521.604 kb on + strand at 521.604 kb on + strand at 521.608 kb on + strand at 521.608 kb on + strand at 521.608 kb on + strand at 521.608 kb on + strand at 521.608 kb on + strand at 521.608 kb on + strand at 521.609 kb on - strand at 521.610 kb on + strand at 521.610 kb on + strand at 521.610 kb on + strand at 521.610 kb on + strand at 521.610 kb on + strand at 521.611 kb on - strand at 521.668 kb on + strand at 521.668 kb on + strand at 521.694 kb on + strand at 521.694 kb on + strand at 521.694 kb on + strand at 521.694 kb on + strand at 521.695 kb on - strand at 521.728 kb on + strand at 521.728 kb on + strand at 521.729 kb on - strand at 521.735 kb on + strand at 521.736 kb on - strand at 521.750 kb on + strand at 521.774 kb on + strand at 521.775 kb on - strand at 521.802 kb on + strand at 521.803 kb on - strand at 521.828 kb on - strand at 521.829 kb on + strand at 521.829 kb on + strand at 521.830 kb on - strand at 521.830 kb on - strand at 521.830 kb on - strand at 521.830 kb on - strand at 521.833 kb on + strand at 521.834 kb on - strand at 521.834 kb on - strand at 521.882 kb on + strand at 521.883 kb on - strand at 521.929 kb on + strand at 521.990 kb on - strand at 522.021 kb on - strand at 522.021 kb on - strand at 522.080 kb on + strand at 522.081 kb on - strand at 522.081 kb on - strand at 522.116 kb on + strand at 522.116 kb on + strand at 522.116 kb on + strand at 522.117 kb on - strand at 522.182 kb on + strand, within Pf1N1B4_440 at 522.204 kb on + strand, within Pf1N1B4_440 at 522.226 kb on - strand, within Pf1N1B4_440 at 522.226 kb on - strand, within Pf1N1B4_440 at 522.282 kb on + strand at 522.351 kb on + strand, within Pf1N1B4_440 at 522.351 kb on + strand, within Pf1N1B4_440 at 522.351 kb on + strand, within Pf1N1B4_440 at 522.351 kb on + strand, within Pf1N1B4_440 at 522.352 kb on - strand, within Pf1N1B4_440 at 522.352 kb on - strand, within Pf1N1B4_440 at 522.352 kb on - strand, within Pf1N1B4_440 at 522.396 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 520,288 - +1.1 520,382 - +0.1 520,795 + +2.3 520,904 + +1.0 520,956 + -0.0 521,017 - -0.0 521,049 + -0.0 521,049 + -0.0 521,050 - -1.0 521,050 - +0.5 521,050 - -0.0 521,050 - -0.0 521,145 + -0.8 521,145 + +0.5 521,145 + -0.5 521,148 + +1.8 521,148 + -0.8 521,149 - +1.3 521,149 - -0.0 521,181 + -0.0 521,182 - +0.2 521,202 + -0.4 521,202 + -0.3 521,203 - +1.3 521,203 - +0.5 521,203 - -1.0 521,273 - +1.2 521,357 + Pf1N1B4_439 0.44 -1.0 521,357 + Pf1N1B4_439 0.44 +0.4 521,357 + Pf1N1B4_439 0.44 +2.3 521,357 + Pf1N1B4_439 0.44 +2.1 521,358 - Pf1N1B4_439 0.44 -1.0 521,358 - Pf1N1B4_439 0.44 -0.0 521,358 - Pf1N1B4_439 0.44 -0.0 521,358 - Pf1N1B4_439 0.44 -0.5 521,358 - Pf1N1B4_439 0.44 -1.6 521,363 + Pf1N1B4_439 0.47 +0.1 521,363 + Pf1N1B4_439 0.47 +1.5 521,363 + Pf1N1B4_439 0.47 +0.4 521,364 - Pf1N1B4_439 0.47 -0.0 521,364 - Pf1N1B4_439 0.47 +2.0 521,403 - Pf1N1B4_439 0.68 -0.0 521,403 - Pf1N1B4_439 0.68 +2.0 521,406 - Pf1N1B4_439 0.69 +1.4 521,444 + Pf1N1B4_439 0.89 +0.7 521,444 + Pf1N1B4_439 0.89 +2.0 521,444 + Pf1N1B4_439 0.89 +1.0 521,444 + Pf1N1B4_439 0.89 +0.7 521,445 - +1.0 521,463 - +2.3 521,463 - -1.6 521,533 + +1.3 521,534 - -0.0 521,534 - +2.3 521,571 + -3.0 521,604 + +1.5 521,604 + -0.6 521,604 + -1.0 521,604 + -1.3 521,608 + +1.4 521,608 + -1.0 521,608 + +0.4 521,608 + -0.9 521,608 + -0.2 521,608 + -1.0 521,609 - +0.5 521,610 + -0.6 521,610 + +1.0 521,610 + +1.4 521,610 + +0.6 521,610 + -0.0 521,611 - +0.5 521,668 + +2.0 521,668 + -0.1 521,694 + +2.0 521,694 + -0.5 521,694 + +0.4 521,694 + +1.5 521,695 - +0.6 521,728 + -1.4 521,728 + +0.5 521,729 - -1.0 521,735 + +1.0 521,736 - -0.6 521,750 + +0.7 521,774 + -2.0 521,775 - -0.9 521,802 + -0.8 521,803 - -0.8 521,828 - -1.0 521,829 + -0.5 521,829 + -0.6 521,830 - +0.1 521,830 - -0.6 521,830 - -0.1 521,830 - +0.5 521,833 + -1.6 521,834 - -1.0 521,834 - -0.0 521,882 + -0.0 521,883 - +3.0 521,929 + +2.8 521,990 - -0.0 522,021 - +0.5 522,021 - +1.0 522,080 + -0.0 522,081 - -2.4 522,081 - -0.0 522,116 + -0.0 522,116 + +0.7 522,116 + -2.4 522,117 - +1.0 522,182 + Pf1N1B4_440 0.17 +2.8 522,204 + Pf1N1B4_440 0.26 +0.4 522,226 - Pf1N1B4_440 0.35 +0.6 522,226 - Pf1N1B4_440 0.35 -0.5 522,282 + +0.5 522,351 + Pf1N1B4_440 0.86 -2.0 522,351 + Pf1N1B4_440 0.86 +0.5 522,351 + Pf1N1B4_440 0.86 +1.3 522,351 + Pf1N1B4_440 0.86 -0.4 522,352 - Pf1N1B4_440 0.87 -0.9 522,352 - Pf1N1B4_440 0.87 +1.3 522,352 - Pf1N1B4_440 0.87 +0.2 522,396 + -0.8
Or see this region's nucleotide sequence