Experiment: nitrate 2000 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2104 and Shew_2105 are separated by 379 nucleotides Shew_2105 and Shew_2106 are separated by 48 nucleotides Shew_2106 and Shew_2107 are separated by 229 nucleotides
Shew_2104: Shew_2104 - major facilitator transporter (RefSeq), at 2,435,305 to 2,437,026
_2104
Shew_2105: Shew_2105 - methylated-DNA-(protein)-cysteine S-methyltransferase (RefSeq), at 2,437,406 to 2,437,756
_2105
Shew_2106: Shew_2106 - hypothetical protein (RefSeq), at 2,437,805 to 2,438,596
_2106
Shew_2107: Shew_2107 - glutamine amidotransferase, class-II (RefSeq), at 2,438,826 to 2,439,611
_2107
Position (kb)
2437
2438
2439 Strain fitness (log2 ratio)
-1
0
1
2 at 2436.906 kb on - strand at 2436.906 kb on - strand at 2436.947 kb on + strand at 2437.697 kb on - strand, within Shew_2105 at 2437.750 kb on - strand at 2437.832 kb on - strand at 2438.279 kb on - strand, within Shew_2106 at 2438.294 kb on - strand, within Shew_2106 at 2438.339 kb on - strand, within Shew_2106 at 2438.357 kb on + strand, within Shew_2106 at 2438.477 kb on + strand, within Shew_2106 at 2438.509 kb on - strand, within Shew_2106 at 2438.554 kb on + strand at 2438.719 kb on - strand at 2438.719 kb on - strand at 2438.750 kb on - strand at 2438.848 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 2000 mM remove 2,436,906 - +0.3 2,436,906 - +0.1 2,436,947 + +0.5 2,437,697 - Shew_2105 0.83 -0.0 2,437,750 - +0.3 2,437,832 - +0.7 2,438,279 - Shew_2106 0.60 +0.7 2,438,294 - Shew_2106 0.62 +2.3 2,438,339 - Shew_2106 0.67 +1.0 2,438,357 + Shew_2106 0.70 -0.0 2,438,477 + Shew_2106 0.85 +0.3 2,438,509 - Shew_2106 0.89 +1.8 2,438,554 + +0.3 2,438,719 - -0.5 2,438,719 - -1.3 2,438,750 - -0.3 2,438,848 + +0.6
Or see this region's nucleotide sequence