Experiment: nitrite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2060 and Shew_2061 are separated by 141 nucleotides Shew_2061 and Shew_2062 are separated by 184 nucleotides Shew_2062 and Shew_2063 overlap by 7 nucleotides Shew_2063 and Shew_2064 are separated by 126 nucleotides
Shew_2060: Shew_2060 - pyridoxal-5'-phosphate-dependent enzyme, beta subunit (RefSeq), at 2,379,396 to 2,380,457
_2060
Shew_2061: Shew_2061 - radical SAM domain-containing protein (RefSeq), at 2,380,599 to 2,381,267
_2061
Shew_2062: Shew_2062 - exsB protein (RefSeq), at 2,381,452 to 2,382,135
_2062
Shew_2063: Shew_2063 - rhomboid family protein (RefSeq), at 2,382,129 to 2,382,713
_2063
Shew_2064: Shew_2064 - excinuclease ABC subunit B (RefSeq), at 2,382,840 to 2,384,858
_2064
Position (kb)
2381
2382
2383 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2381.019 kb on + strand, within Shew_2061 at 2381.118 kb on + strand, within Shew_2061 at 2381.331 kb on - strand at 2381.663 kb on + strand, within Shew_2062 at 2381.730 kb on + strand, within Shew_2062 at 2381.978 kb on + strand, within Shew_2062 at 2382.216 kb on - strand, within Shew_2063 at 2382.559 kb on + strand, within Shew_2063 at 2383.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 200 mM remove 2,381,019 + Shew_2061 0.63 +0.9 2,381,118 + Shew_2061 0.78 +1.2 2,381,331 - +0.6 2,381,663 + Shew_2062 0.31 -0.5 2,381,730 + Shew_2062 0.41 +0.9 2,381,978 + Shew_2062 0.77 -1.7 2,382,216 - Shew_2063 0.15 -3.0 2,382,559 + Shew_2063 0.74 -2.7 2,383,002 - -0.2
Or see this region's nucleotide sequence