Experiment: R2A with Lomefloxacin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS06605 and CA265_RS06610 are separated by 190 nucleotides CA265_RS06610 and CA265_RS06615 are separated by 79 nucleotides CA265_RS06615 and CA265_RS06620 are separated by 101 nucleotides CA265_RS06620 and CA265_RS06625 are separated by 47 nucleotides
CA265_RS06605: CA265_RS06605 - hypothetical protein, at 1,632,136 to 1,632,993
_RS06605
CA265_RS06610: CA265_RS06610 - transmembrane HD family protein, at 1,633,184 to 1,635,190
_RS06610
CA265_RS06615: CA265_RS06615 - tRNA-Ser, at 1,635,270 to 1,635,356
_RS06615
CA265_RS06620: CA265_RS06620 - tRNA-Pro, at 1,635,458 to 1,635,534
_RS06620
CA265_RS06625: CA265_RS06625 - tRNA-Pro, at 1,635,582 to 1,635,655
_RS06625
Position (kb)
1633
1634
1635
1636 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1632.202 kb on + strand at 1632.203 kb on - strand at 1632.262 kb on + strand, within CA265_RS06605 at 1632.284 kb on - strand, within CA265_RS06605 at 1632.293 kb on - strand, within CA265_RS06605 at 1632.305 kb on - strand, within CA265_RS06605 at 1632.380 kb on + strand, within CA265_RS06605 at 1632.417 kb on + strand, within CA265_RS06605 at 1632.568 kb on + strand, within CA265_RS06605 at 1632.569 kb on - strand, within CA265_RS06605 at 1632.569 kb on - strand, within CA265_RS06605 at 1632.569 kb on - strand, within CA265_RS06605 at 1632.574 kb on + strand, within CA265_RS06605 at 1632.575 kb on - strand, within CA265_RS06605 at 1632.578 kb on + strand, within CA265_RS06605 at 1632.578 kb on + strand, within CA265_RS06605 at 1632.588 kb on - strand, within CA265_RS06605 at 1632.612 kb on + strand, within CA265_RS06605 at 1632.685 kb on + strand, within CA265_RS06605 at 1632.748 kb on + strand, within CA265_RS06605 at 1632.783 kb on + strand, within CA265_RS06605 at 1632.785 kb on + strand, within CA265_RS06605 at 1632.801 kb on + strand, within CA265_RS06605 at 1632.801 kb on + strand, within CA265_RS06605 at 1632.801 kb on + strand, within CA265_RS06605 at 1632.802 kb on - strand, within CA265_RS06605 at 1632.802 kb on - strand, within CA265_RS06605 at 1632.857 kb on - strand, within CA265_RS06605 at 1632.862 kb on + strand, within CA265_RS06605 at 1632.863 kb on - strand, within CA265_RS06605 at 1632.940 kb on + strand at 1632.953 kb on - strand at 1633.064 kb on + strand at 1633.065 kb on - strand at 1633.066 kb on + strand at 1633.067 kb on - strand at 1633.074 kb on - strand at 1633.094 kb on + strand at 1633.099 kb on + strand at 1633.103 kb on + strand at 1633.158 kb on - strand at 1633.175 kb on + strand at 1633.175 kb on + strand at 1633.208 kb on - strand at 1633.327 kb on + strand at 1633.351 kb on - strand at 1633.475 kb on + strand, within CA265_RS06610 at 1633.478 kb on - strand, within CA265_RS06610 at 1633.491 kb on - strand, within CA265_RS06610 at 1633.492 kb on + strand, within CA265_RS06610 at 1633.507 kb on + strand at 1633.508 kb on - strand, within CA265_RS06610 at 1633.526 kb on - strand, within CA265_RS06610 at 1633.534 kb on - strand, within CA265_RS06610 at 1633.761 kb on + strand, within CA265_RS06610 at 1633.772 kb on - strand, within CA265_RS06610 at 1633.783 kb on + strand, within CA265_RS06610 at 1633.784 kb on - strand, within CA265_RS06610 at 1633.822 kb on + strand, within CA265_RS06610 at 1633.822 kb on + strand, within CA265_RS06610 at 1633.843 kb on + strand, within CA265_RS06610 at 1633.843 kb on + strand, within CA265_RS06610 at 1633.844 kb on - strand, within CA265_RS06610 at 1633.877 kb on + strand, within CA265_RS06610 at 1633.890 kb on - strand, within CA265_RS06610 at 1633.931 kb on + strand, within CA265_RS06610 at 1633.963 kb on - strand, within CA265_RS06610 at 1633.963 kb on - strand, within CA265_RS06610 at 1633.963 kb on - strand, within CA265_RS06610 at 1633.975 kb on - strand, within CA265_RS06610 at 1634.061 kb on + strand, within CA265_RS06610 at 1634.083 kb on + strand, within CA265_RS06610 at 1634.083 kb on + strand, within CA265_RS06610 at 1634.105 kb on + strand, within CA265_RS06610 at 1634.109 kb on - strand, within CA265_RS06610 at 1634.131 kb on + strand, within CA265_RS06610 at 1634.132 kb on - strand, within CA265_RS06610 at 1634.135 kb on - strand, within CA265_RS06610 at 1634.180 kb on - strand, within CA265_RS06610 at 1634.180 kb on - strand, within CA265_RS06610 at 1634.269 kb on + strand, within CA265_RS06610 at 1634.269 kb on + strand, within CA265_RS06610 at 1634.270 kb on - strand, within CA265_RS06610 at 1634.270 kb on - strand, within CA265_RS06610 at 1634.353 kb on + strand, within CA265_RS06610 at 1634.355 kb on + strand, within CA265_RS06610 at 1634.457 kb on - strand, within CA265_RS06610 at 1634.522 kb on - strand, within CA265_RS06610 at 1634.623 kb on + strand, within CA265_RS06610 at 1634.624 kb on - strand, within CA265_RS06610 at 1634.654 kb on + strand, within CA265_RS06610 at 1634.729 kb on - strand, within CA265_RS06610 at 1634.734 kb on - strand, within CA265_RS06610 at 1635.031 kb on - strand at 1635.056 kb on - strand at 1635.056 kb on - strand at 1635.056 kb on - strand at 1635.056 kb on - strand at 1635.152 kb on - strand at 1635.156 kb on - strand at 1635.168 kb on + strand at 1635.169 kb on - strand at 1635.590 kb on - strand, within CA265_RS06625 at 1635.676 kb on + strand at 1635.677 kb on - strand at 1635.685 kb on - strand at 1635.837 kb on - strand at 1635.851 kb on - strand at 1635.856 kb on - strand at 1635.913 kb on - strand at 1635.949 kb on + strand at 1635.949 kb on + strand at 1635.950 kb on - strand at 1635.951 kb on + strand at 1635.951 kb on + strand at 1635.951 kb on + strand at 1635.952 kb on - strand at 1635.958 kb on - strand at 1636.005 kb on + strand at 1636.037 kb on - strand at 1636.050 kb on + strand at 1636.050 kb on + strand at 1636.051 kb on - strand at 1636.081 kb on + strand at 1636.092 kb on + strand at 1636.093 kb on - strand at 1636.136 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.1 mM remove 1,632,202 + +0.7 1,632,203 - +0.0 1,632,262 + CA265_RS06605 0.15 +1.2 1,632,284 - CA265_RS06605 0.17 -0.2 1,632,293 - CA265_RS06605 0.18 -0.0 1,632,305 - CA265_RS06605 0.20 +0.5 1,632,380 + CA265_RS06605 0.28 +0.8 1,632,417 + CA265_RS06605 0.33 +0.2 1,632,568 + CA265_RS06605 0.50 +0.3 1,632,569 - CA265_RS06605 0.50 -0.9 1,632,569 - CA265_RS06605 0.50 +0.1 1,632,569 - CA265_RS06605 0.50 -1.2 1,632,574 + CA265_RS06605 0.51 -0.1 1,632,575 - CA265_RS06605 0.51 +0.8 1,632,578 + CA265_RS06605 0.52 -0.4 1,632,578 + CA265_RS06605 0.52 +0.2 1,632,588 - CA265_RS06605 0.53 +0.3 1,632,612 + CA265_RS06605 0.55 -1.1 1,632,685 + CA265_RS06605 0.64 -0.6 1,632,748 + CA265_RS06605 0.71 -0.1 1,632,783 + CA265_RS06605 0.75 -0.1 1,632,785 + CA265_RS06605 0.76 +0.3 1,632,801 + CA265_RS06605 0.78 +0.5 1,632,801 + CA265_RS06605 0.78 +0.1 1,632,801 + CA265_RS06605 0.78 +0.0 1,632,802 - CA265_RS06605 0.78 -0.0 1,632,802 - CA265_RS06605 0.78 +0.6 1,632,857 - CA265_RS06605 0.84 -0.5 1,632,862 + CA265_RS06605 0.85 +0.5 1,632,863 - CA265_RS06605 0.85 -0.5 1,632,940 + -0.1 1,632,953 - -0.0 1,633,064 + -1.4 1,633,065 - +1.1 1,633,066 + -0.4 1,633,067 - -0.1 1,633,074 - -0.3 1,633,094 + -0.5 1,633,099 + -1.7 1,633,103 + -0.8 1,633,158 - -0.9 1,633,175 + -0.9 1,633,175 + -2.8 1,633,208 - -3.8 1,633,327 + +0.3 1,633,351 - -1.7 1,633,475 + CA265_RS06610 0.14 +0.3 1,633,478 - CA265_RS06610 0.15 -2.7 1,633,491 - CA265_RS06610 0.15 -1.9 1,633,492 + CA265_RS06610 0.15 -0.8 1,633,507 + +0.6 1,633,508 - CA265_RS06610 0.16 -1.8 1,633,526 - CA265_RS06610 0.17 -0.5 1,633,534 - CA265_RS06610 0.17 -0.9 1,633,761 + CA265_RS06610 0.29 -1.5 1,633,772 - CA265_RS06610 0.29 -1.0 1,633,783 + CA265_RS06610 0.30 -1.0 1,633,784 - CA265_RS06610 0.30 -1.6 1,633,822 + CA265_RS06610 0.32 -1.9 1,633,822 + CA265_RS06610 0.32 -2.3 1,633,843 + CA265_RS06610 0.33 -1.1 1,633,843 + CA265_RS06610 0.33 -1.4 1,633,844 - CA265_RS06610 0.33 -1.2 1,633,877 + CA265_RS06610 0.35 -1.1 1,633,890 - CA265_RS06610 0.35 -1.4 1,633,931 + CA265_RS06610 0.37 -0.5 1,633,963 - CA265_RS06610 0.39 -1.2 1,633,963 - CA265_RS06610 0.39 -2.1 1,633,963 - CA265_RS06610 0.39 -1.0 1,633,975 - CA265_RS06610 0.39 -3.1 1,634,061 + CA265_RS06610 0.44 -0.0 1,634,083 + CA265_RS06610 0.45 -1.6 1,634,083 + CA265_RS06610 0.45 -1.4 1,634,105 + CA265_RS06610 0.46 -0.9 1,634,109 - CA265_RS06610 0.46 -1.2 1,634,131 + CA265_RS06610 0.47 -0.5 1,634,132 - CA265_RS06610 0.47 -1.6 1,634,135 - CA265_RS06610 0.47 -3.2 1,634,180 - CA265_RS06610 0.50 -2.2 1,634,180 - CA265_RS06610 0.50 -1.7 1,634,269 + CA265_RS06610 0.54 -1.2 1,634,269 + CA265_RS06610 0.54 -1.9 1,634,270 - CA265_RS06610 0.54 -1.0 1,634,270 - CA265_RS06610 0.54 -0.9 1,634,353 + CA265_RS06610 0.58 -0.4 1,634,355 + CA265_RS06610 0.58 -0.0 1,634,457 - CA265_RS06610 0.63 -0.4 1,634,522 - CA265_RS06610 0.67 -1.5 1,634,623 + CA265_RS06610 0.72 -1.4 1,634,624 - CA265_RS06610 0.72 -1.1 1,634,654 + CA265_RS06610 0.73 -1.2 1,634,729 - CA265_RS06610 0.77 -0.8 1,634,734 - CA265_RS06610 0.77 +0.6 1,635,031 - -1.3 1,635,056 - -1.3 1,635,056 - -0.4 1,635,056 - -2.2 1,635,056 - -1.1 1,635,152 - -1.7 1,635,156 - -1.1 1,635,168 + -0.5 1,635,169 - -2.2 1,635,590 - CA265_RS06625 0.11 -2.2 1,635,676 + +0.4 1,635,677 - +0.0 1,635,685 - -0.3 1,635,837 - +1.0 1,635,851 - +0.6 1,635,856 - -0.4 1,635,913 - +0.2 1,635,949 + -0.2 1,635,949 + -0.6 1,635,950 - +0.4 1,635,951 + -0.1 1,635,951 + +0.2 1,635,951 + -0.2 1,635,952 - -0.1 1,635,958 - -1.6 1,636,005 + -0.4 1,636,037 - +0.9 1,636,050 + -0.7 1,636,050 + -0.1 1,636,051 - -1.6 1,636,081 + +0.3 1,636,092 + -0.2 1,636,093 - -0.2 1,636,136 + -1.2
Or see this region's nucleotide sequence