Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2060 and Shew_2061 are separated by 141 nucleotides Shew_2061 and Shew_2062 are separated by 184 nucleotides Shew_2062 and Shew_2063 overlap by 7 nucleotides
Shew_2060: Shew_2060 - pyridoxal-5'-phosphate-dependent enzyme, beta subunit (RefSeq), at 2,379,396 to 2,380,457
_2060
Shew_2061: Shew_2061 - radical SAM domain-containing protein (RefSeq), at 2,380,599 to 2,381,267
_2061
Shew_2062: Shew_2062 - exsB protein (RefSeq), at 2,381,452 to 2,382,135
_2062
Shew_2063: Shew_2063 - rhomboid family protein (RefSeq), at 2,382,129 to 2,382,713
_2063
Position (kb)
2380
2381
2382 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2379.908 kb on + strand, within Shew_2060 at 2381.019 kb on + strand, within Shew_2061 at 2381.118 kb on + strand, within Shew_2061 at 2381.331 kb on - strand at 2381.663 kb on + strand, within Shew_2062 at 2381.730 kb on + strand, within Shew_2062 at 2381.978 kb on + strand, within Shew_2062 at 2382.216 kb on - strand, within Shew_2063
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,379,908 + Shew_2060 0.48 -3.6 2,381,019 + Shew_2061 0.63 -0.8 2,381,118 + Shew_2061 0.78 +1.0 2,381,331 - -0.3 2,381,663 + Shew_2062 0.31 -1.9 2,381,730 + Shew_2062 0.41 -1.5 2,381,978 + Shew_2062 0.77 -3.1 2,382,216 - Shew_2063 0.15 -1.8
Or see this region's nucleotide sequence