Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS19595 and CA265_RS19600 are separated by 201 nucleotides CA265_RS19600 and CA265_RS19605 are separated by 68 nucleotides CA265_RS19605 and CA265_RS19610 are separated by 255 nucleotides CA265_RS19610 and CA265_RS19615 are separated by 77 nucleotides
CA265_RS19595: CA265_RS19595 - GxxExxY protein, at 4,857,407 to 4,857,784
_RS19595
CA265_RS19600: CA265_RS19600 - hypothetical protein, at 4,857,986 to 4,858,537
_RS19600
CA265_RS19605: CA265_RS19605 - serine hydrolase, at 4,858,606 to 4,859,838
_RS19605
CA265_RS19610: CA265_RS19610 - hypothetical protein, at 4,860,094 to 4,860,399
_RS19610
CA265_RS19615: CA265_RS19615 - metal-dependent hydrolase, at 4,860,477 to 4,861,154
_RS19615
Position (kb)
4858
4859
4860 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 4857.606 kb on - strand, within CA265_RS19595 at 4857.612 kb on - strand, within CA265_RS19595 at 4857.613 kb on + strand, within CA265_RS19595 at 4857.632 kb on + strand, within CA265_RS19595 at 4857.678 kb on + strand, within CA265_RS19595 at 4857.723 kb on - strand, within CA265_RS19595 at 4857.728 kb on - strand, within CA265_RS19595 at 4857.795 kb on - strand at 4857.803 kb on - strand at 4857.825 kb on + strand at 4857.846 kb on - strand at 4857.886 kb on + strand at 4857.956 kb on + strand at 4858.093 kb on + strand, within CA265_RS19600 at 4858.158 kb on - strand, within CA265_RS19600 at 4858.160 kb on - strand, within CA265_RS19600 at 4858.160 kb on - strand, within CA265_RS19600 at 4858.160 kb on - strand, within CA265_RS19600 at 4858.173 kb on + strand, within CA265_RS19600 at 4858.180 kb on + strand, within CA265_RS19600 at 4858.199 kb on + strand, within CA265_RS19600 at 4858.231 kb on - strand, within CA265_RS19600 at 4858.252 kb on - strand, within CA265_RS19600 at 4858.252 kb on - strand, within CA265_RS19600 at 4858.279 kb on + strand, within CA265_RS19600 at 4858.280 kb on - strand, within CA265_RS19600 at 4858.323 kb on + strand, within CA265_RS19600 at 4858.324 kb on - strand, within CA265_RS19600 at 4858.386 kb on + strand, within CA265_RS19600 at 4858.391 kb on + strand, within CA265_RS19600 at 4858.398 kb on + strand, within CA265_RS19600 at 4858.399 kb on - strand, within CA265_RS19600 at 4858.498 kb on - strand at 4858.592 kb on + strand at 4858.602 kb on - strand at 4858.602 kb on - strand at 4858.620 kb on + strand at 4858.620 kb on + strand at 4858.620 kb on + strand at 4858.621 kb on - strand at 4858.622 kb on + strand at 4858.622 kb on + strand at 4858.753 kb on + strand, within CA265_RS19605 at 4858.821 kb on + strand, within CA265_RS19605 at 4858.831 kb on - strand, within CA265_RS19605 at 4858.888 kb on + strand, within CA265_RS19605 at 4858.915 kb on - strand, within CA265_RS19605 at 4858.980 kb on - strand, within CA265_RS19605 at 4859.060 kb on + strand, within CA265_RS19605 at 4859.093 kb on - strand, within CA265_RS19605 at 4859.106 kb on - strand, within CA265_RS19605 at 4859.112 kb on + strand, within CA265_RS19605 at 4859.113 kb on - strand, within CA265_RS19605 at 4859.165 kb on + strand, within CA265_RS19605 at 4859.201 kb on - strand, within CA265_RS19605 at 4859.233 kb on - strand, within CA265_RS19605 at 4859.274 kb on - strand, within CA265_RS19605 at 4859.296 kb on + strand, within CA265_RS19605 at 4859.297 kb on + strand, within CA265_RS19605 at 4859.338 kb on - strand, within CA265_RS19605 at 4859.338 kb on - strand, within CA265_RS19605 at 4859.465 kb on - strand at 4859.519 kb on + strand, within CA265_RS19605 at 4859.549 kb on - strand, within CA265_RS19605 at 4859.588 kb on - strand, within CA265_RS19605 at 4859.674 kb on + strand, within CA265_RS19605 at 4859.675 kb on - strand, within CA265_RS19605 at 4859.707 kb on - strand, within CA265_RS19605 at 4859.728 kb on - strand at 4859.840 kb on + strand at 4859.845 kb on + strand at 4859.924 kb on + strand at 4859.925 kb on - strand at 4859.925 kb on - strand at 4859.975 kb on - strand at 4859.977 kb on - strand at 4860.014 kb on - strand at 4860.035 kb on + strand at 4860.040 kb on - strand at 4860.075 kb on + strand at 4860.205 kb on - strand, within CA265_RS19610 at 4860.363 kb on + strand, within CA265_RS19610 at 4860.364 kb on - strand, within CA265_RS19610 at 4860.458 kb on - strand at 4860.477 kb on - strand at 4860.484 kb on - strand at 4860.489 kb on + strand at 4860.489 kb on + strand at 4860.489 kb on + strand at 4860.490 kb on - strand at 4860.554 kb on - strand, within CA265_RS19615 at 4860.568 kb on + strand, within CA265_RS19615 at 4860.573 kb on - strand, within CA265_RS19615 at 4860.588 kb on - strand, within CA265_RS19615 at 4860.609 kb on - strand, within CA265_RS19615 at 4860.609 kb on - strand, within CA265_RS19615 at 4860.658 kb on + strand, within CA265_RS19615 at 4860.683 kb on + strand, within CA265_RS19615 at 4860.684 kb on - strand, within CA265_RS19615 at 4860.684 kb on - strand, within CA265_RS19615 at 4860.686 kb on - strand, within CA265_RS19615 at 4860.700 kb on + strand, within CA265_RS19615 at 4860.716 kb on + strand, within CA265_RS19615
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 4,857,606 - CA265_RS19595 0.53 +0.4 4,857,612 - CA265_RS19595 0.54 -0.3 4,857,613 + CA265_RS19595 0.54 +0.8 4,857,632 + CA265_RS19595 0.60 +1.0 4,857,678 + CA265_RS19595 0.72 -1.9 4,857,723 - CA265_RS19595 0.84 -0.5 4,857,728 - CA265_RS19595 0.85 +1.0 4,857,795 - +1.2 4,857,803 - -1.2 4,857,825 + +0.5 4,857,846 - -1.5 4,857,886 + +0.1 4,857,956 + -0.1 4,858,093 + CA265_RS19600 0.19 +3.0 4,858,158 - CA265_RS19600 0.31 +0.7 4,858,160 - CA265_RS19600 0.32 +0.9 4,858,160 - CA265_RS19600 0.32 +0.9 4,858,160 - CA265_RS19600 0.32 +0.5 4,858,173 + CA265_RS19600 0.34 -0.1 4,858,180 + CA265_RS19600 0.35 +2.2 4,858,199 + CA265_RS19600 0.39 +2.9 4,858,231 - CA265_RS19600 0.44 +0.4 4,858,252 - CA265_RS19600 0.48 +1.2 4,858,252 - CA265_RS19600 0.48 +0.2 4,858,279 + CA265_RS19600 0.53 +3.0 4,858,280 - CA265_RS19600 0.53 +0.7 4,858,323 + CA265_RS19600 0.61 +1.9 4,858,324 - CA265_RS19600 0.61 +0.8 4,858,386 + CA265_RS19600 0.72 +1.2 4,858,391 + CA265_RS19600 0.73 -1.4 4,858,398 + CA265_RS19600 0.75 +1.8 4,858,399 - CA265_RS19600 0.75 -0.4 4,858,498 - -0.8 4,858,592 + +2.0 4,858,602 - +5.2 4,858,602 - +4.9 4,858,620 + +5.4 4,858,620 + +5.9 4,858,620 + +5.3 4,858,621 - +4.9 4,858,622 + +6.0 4,858,622 + +5.1 4,858,753 + CA265_RS19605 0.12 +4.5 4,858,821 + CA265_RS19605 0.17 +5.6 4,858,831 - CA265_RS19605 0.18 +4.9 4,858,888 + CA265_RS19605 0.23 +3.3 4,858,915 - CA265_RS19605 0.25 +5.5 4,858,980 - CA265_RS19605 0.30 +5.4 4,859,060 + CA265_RS19605 0.37 +4.2 4,859,093 - CA265_RS19605 0.39 +5.2 4,859,106 - CA265_RS19605 0.41 +4.6 4,859,112 + CA265_RS19605 0.41 +1.4 4,859,113 - CA265_RS19605 0.41 +5.2 4,859,165 + CA265_RS19605 0.45 +4.7 4,859,201 - CA265_RS19605 0.48 +5.5 4,859,233 - CA265_RS19605 0.51 +5.6 4,859,274 - CA265_RS19605 0.54 +5.2 4,859,296 + CA265_RS19605 0.56 +3.8 4,859,297 + CA265_RS19605 0.56 +4.2 4,859,338 - CA265_RS19605 0.59 +5.3 4,859,338 - CA265_RS19605 0.59 +5.4 4,859,465 - +3.8 4,859,519 + CA265_RS19605 0.74 +3.6 4,859,549 - CA265_RS19605 0.76 +5.2 4,859,588 - CA265_RS19605 0.80 +4.8 4,859,674 + CA265_RS19605 0.87 +3.7 4,859,675 - CA265_RS19605 0.87 +4.5 4,859,707 - CA265_RS19605 0.89 +5.2 4,859,728 - +4.9 4,859,840 + +0.4 4,859,845 + -0.4 4,859,924 + +0.9 4,859,925 - +5.1 4,859,925 - +5.2 4,859,975 - +3.9 4,859,977 - +5.2 4,860,014 - +4.7 4,860,035 + -0.0 4,860,040 - +4.0 4,860,075 + +0.4 4,860,205 - CA265_RS19610 0.36 +2.0 4,860,363 + CA265_RS19610 0.88 +2.0 4,860,364 - CA265_RS19610 0.88 +2.1 4,860,458 - +0.4 4,860,477 - +1.5 4,860,484 - +1.5 4,860,489 + +1.0 4,860,489 + +1.1 4,860,489 + -0.4 4,860,490 - +1.3 4,860,554 - CA265_RS19615 0.11 -0.2 4,860,568 + CA265_RS19615 0.13 -1.0 4,860,573 - CA265_RS19615 0.14 +2.8 4,860,588 - CA265_RS19615 0.16 +0.7 4,860,609 - CA265_RS19615 0.19 +1.0 4,860,609 - CA265_RS19615 0.19 +2.7 4,860,658 + CA265_RS19615 0.27 -0.2 4,860,683 + CA265_RS19615 0.30 +0.9 4,860,684 - CA265_RS19615 0.31 +1.2 4,860,684 - CA265_RS19615 0.31 +0.7 4,860,686 - CA265_RS19615 0.31 +0.9 4,860,700 + CA265_RS19615 0.33 +0.8 4,860,716 + CA265_RS19615 0.35 +0.4
Or see this region's nucleotide sequence