Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14975 and CA265_RS14980 are separated by 346 nucleotides CA265_RS14980 and CA265_RS14985 are separated by 204 nucleotides CA265_RS14985 and CA265_RS14990 are separated by 240 nucleotides
CA265_RS14975: CA265_RS14975 - ammonia channel protein, at 3,773,603 to 3,775,009
_RS14975
CA265_RS14980: CA265_RS14980 - TIGR01212 family radical SAM protein, at 3,775,356 to 3,776,303
_RS14980
CA265_RS14985: CA265_RS14985 - hypothetical protein, at 3,776,508 to 3,776,690
_RS14985
CA265_RS14990: CA265_RS14990 - amino acid transporter, at 3,776,931 to 3,778,373
_RS14990
Position (kb)
3775
3776
3777 Strain fitness (log2 ratio)
-2
-1
0
1 at 3774.392 kb on + strand, within CA265_RS14975 at 3774.420 kb on - strand, within CA265_RS14975 at 3774.514 kb on - strand, within CA265_RS14975 at 3774.514 kb on - strand, within CA265_RS14975 at 3774.565 kb on + strand, within CA265_RS14975 at 3774.565 kb on + strand, within CA265_RS14975 at 3774.665 kb on - strand, within CA265_RS14975 at 3774.791 kb on - strand, within CA265_RS14975 at 3774.852 kb on - strand, within CA265_RS14975 at 3775.412 kb on + strand at 3775.497 kb on - strand, within CA265_RS14980 at 3775.568 kb on + strand, within CA265_RS14980 at 3775.568 kb on + strand, within CA265_RS14980 at 3775.568 kb on + strand, within CA265_RS14980 at 3775.568 kb on + strand, within CA265_RS14980 at 3775.614 kb on + strand, within CA265_RS14980 at 3775.615 kb on - strand, within CA265_RS14980 at 3775.673 kb on + strand, within CA265_RS14980 at 3775.673 kb on + strand, within CA265_RS14980 at 3775.675 kb on + strand, within CA265_RS14980 at 3775.743 kb on - strand, within CA265_RS14980 at 3775.823 kb on - strand, within CA265_RS14980 at 3775.827 kb on - strand, within CA265_RS14980 at 3775.827 kb on - strand, within CA265_RS14980 at 3775.870 kb on + strand, within CA265_RS14980 at 3775.871 kb on - strand, within CA265_RS14980 at 3775.941 kb on - strand, within CA265_RS14980 at 3775.972 kb on + strand, within CA265_RS14980 at 3775.984 kb on - strand, within CA265_RS14980 at 3776.006 kb on + strand, within CA265_RS14980 at 3776.034 kb on - strand, within CA265_RS14980 at 3776.035 kb on + strand, within CA265_RS14980 at 3776.036 kb on - strand, within CA265_RS14980 at 3776.036 kb on - strand, within CA265_RS14980 at 3776.036 kb on - strand, within CA265_RS14980 at 3776.040 kb on - strand, within CA265_RS14980 at 3776.146 kb on + strand, within CA265_RS14980 at 3776.147 kb on - strand, within CA265_RS14980 at 3776.151 kb on - strand, within CA265_RS14980 at 3776.205 kb on + strand, within CA265_RS14980 at 3776.212 kb on - strand at 3776.235 kb on - strand at 3776.237 kb on - strand at 3776.304 kb on + strand at 3776.356 kb on + strand at 3776.399 kb on + strand at 3776.400 kb on - strand at 3776.727 kb on + strand at 3776.751 kb on + strand at 3776.884 kb on + strand at 3776.885 kb on - strand at 3776.896 kb on - strand at 3776.958 kb on - strand at 3777.010 kb on + strand at 3777.010 kb on + strand at 3777.011 kb on - strand at 3777.028 kb on - strand at 3777.060 kb on + strand at 3777.077 kb on + strand, within CA265_RS14990 at 3777.086 kb on - strand, within CA265_RS14990 at 3777.087 kb on + strand, within CA265_RS14990 at 3777.126 kb on + strand, within CA265_RS14990 at 3777.155 kb on + strand, within CA265_RS14990 at 3777.164 kb on - strand, within CA265_RS14990 at 3777.209 kb on - strand, within CA265_RS14990
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 3,774,392 + CA265_RS14975 0.56 -1.2 3,774,420 - CA265_RS14975 0.58 -0.4 3,774,514 - CA265_RS14975 0.65 -0.4 3,774,514 - CA265_RS14975 0.65 -0.3 3,774,565 + CA265_RS14975 0.68 -0.5 3,774,565 + CA265_RS14975 0.68 +0.1 3,774,665 - CA265_RS14975 0.75 -0.1 3,774,791 - CA265_RS14975 0.84 -0.2 3,774,852 - CA265_RS14975 0.89 +0.4 3,775,412 + -0.9 3,775,497 - CA265_RS14980 0.15 -1.5 3,775,568 + CA265_RS14980 0.22 -0.4 3,775,568 + CA265_RS14980 0.22 -1.4 3,775,568 + CA265_RS14980 0.22 -1.0 3,775,568 + CA265_RS14980 0.22 -0.4 3,775,614 + CA265_RS14980 0.27 -1.5 3,775,615 - CA265_RS14980 0.27 -0.3 3,775,673 + CA265_RS14980 0.33 -0.9 3,775,673 + CA265_RS14980 0.33 -1.6 3,775,675 + CA265_RS14980 0.34 -0.9 3,775,743 - CA265_RS14980 0.41 -0.6 3,775,823 - CA265_RS14980 0.49 -0.9 3,775,827 - CA265_RS14980 0.50 -0.1 3,775,827 - CA265_RS14980 0.50 -0.8 3,775,870 + CA265_RS14980 0.54 -0.1 3,775,871 - CA265_RS14980 0.54 -0.4 3,775,941 - CA265_RS14980 0.62 -0.0 3,775,972 + CA265_RS14980 0.65 +0.3 3,775,984 - CA265_RS14980 0.66 -0.6 3,776,006 + CA265_RS14980 0.69 -2.1 3,776,034 - CA265_RS14980 0.72 +0.0 3,776,035 + CA265_RS14980 0.72 -2.4 3,776,036 - CA265_RS14980 0.72 -1.2 3,776,036 - CA265_RS14980 0.72 +1.5 3,776,036 - CA265_RS14980 0.72 -0.5 3,776,040 - CA265_RS14980 0.72 -0.3 3,776,146 + CA265_RS14980 0.83 -0.9 3,776,147 - CA265_RS14980 0.83 -0.6 3,776,151 - CA265_RS14980 0.84 -0.3 3,776,205 + CA265_RS14980 0.90 -0.4 3,776,212 - -1.7 3,776,235 - -1.0 3,776,237 - -0.3 3,776,304 + -0.7 3,776,356 + -0.7 3,776,399 + -0.8 3,776,400 - +0.7 3,776,727 + -1.6 3,776,751 + -0.0 3,776,884 + -0.3 3,776,885 - +0.7 3,776,896 - +0.9 3,776,958 - -1.5 3,777,010 + -0.0 3,777,010 + -0.4 3,777,011 - -0.1 3,777,028 - -0.1 3,777,060 + -0.1 3,777,077 + CA265_RS14990 0.10 -0.5 3,777,086 - CA265_RS14990 0.11 -0.6 3,777,087 + CA265_RS14990 0.11 -0.4 3,777,126 + CA265_RS14990 0.14 +1.1 3,777,155 + CA265_RS14990 0.16 +0.0 3,777,164 - CA265_RS14990 0.16 -1.5 3,777,209 - CA265_RS14990 0.19 -2.0
Or see this region's nucleotide sequence