Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS12070 and CA265_RS12075 are separated by 414 nucleotides CA265_RS12075 and CA265_RS12080 are separated by 364 nucleotides
CA265_RS12070: CA265_RS12070 - SusC/RagA family TonB-linked outer membrane protein, at 2,988,082 to 2,991,216
_RS12070
CA265_RS12075: CA265_RS12075 - hypothetical protein, at 2,991,631 to 2,991,822
_RS12075
CA265_RS12080: CA265_RS12080 - hypothetical protein, at 2,992,187 to 2,992,816
_RS12080
Position (kb)
2991
2992 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2990.658 kb on + strand, within CA265_RS12070 at 2990.672 kb on - strand, within CA265_RS12070 at 2990.796 kb on + strand, within CA265_RS12070 at 2990.818 kb on + strand, within CA265_RS12070 at 2990.894 kb on - strand, within CA265_RS12070 at 2990.938 kb on - strand at 2990.991 kb on + strand at 2990.992 kb on - strand at 2990.994 kb on - strand at 2991.065 kb on - strand at 2991.065 kb on - strand at 2991.065 kb on - strand at 2991.065 kb on - strand at 2991.105 kb on + strand at 2991.106 kb on - strand at 2991.180 kb on + strand at 2991.180 kb on + strand at 2991.221 kb on + strand at 2991.224 kb on - strand at 2991.375 kb on - strand at 2991.407 kb on + strand at 2991.457 kb on + strand at 2991.458 kb on - strand at 2991.481 kb on - strand at 2991.494 kb on - strand at 2991.527 kb on - strand at 2991.546 kb on + strand at 2991.546 kb on + strand at 2991.579 kb on + strand at 2991.580 kb on - strand at 2991.611 kb on + strand at 2991.630 kb on - strand at 2991.740 kb on + strand, within CA265_RS12075 at 2991.741 kb on - strand, within CA265_RS12075 at 2991.741 kb on - strand, within CA265_RS12075 at 2991.744 kb on + strand, within CA265_RS12075 at 2991.831 kb on + strand at 2991.831 kb on + strand at 2991.843 kb on - strand at 2991.916 kb on - strand at 2991.990 kb on - strand at 2992.001 kb on - strand at 2992.014 kb on + strand at 2992.074 kb on + strand at 2992.081 kb on + strand at 2992.085 kb on - strand at 2992.086 kb on + strand at 2992.086 kb on + strand at 2992.168 kb on + strand at 2992.201 kb on + strand at 2992.236 kb on - strand at 2992.236 kb on - strand at 2992.237 kb on + strand at 2992.238 kb on - strand at 2992.238 kb on - strand at 2992.276 kb on + strand, within CA265_RS12080 at 2992.326 kb on - strand at 2992.329 kb on - strand, within CA265_RS12080 at 2992.348 kb on + strand, within CA265_RS12080 at 2992.349 kb on - strand, within CA265_RS12080 at 2992.351 kb on - strand, within CA265_RS12080 at 2992.351 kb on - strand, within CA265_RS12080 at 2992.415 kb on + strand, within CA265_RS12080 at 2992.415 kb on + strand, within CA265_RS12080 at 2992.415 kb on + strand, within CA265_RS12080 at 2992.474 kb on + strand, within CA265_RS12080 at 2992.491 kb on + strand, within CA265_RS12080 at 2992.492 kb on - strand, within CA265_RS12080 at 2992.492 kb on - strand, within CA265_RS12080 at 2992.576 kb on - strand, within CA265_RS12080 at 2992.610 kb on - strand, within CA265_RS12080 at 2992.633 kb on + strand, within CA265_RS12080 at 2992.635 kb on + strand, within CA265_RS12080 at 2992.636 kb on - strand, within CA265_RS12080 at 2992.736 kb on + strand, within CA265_RS12080 at 2992.736 kb on + strand, within CA265_RS12080 at 2992.736 kb on + strand, within CA265_RS12080 at 2992.736 kb on + strand, within CA265_RS12080 at 2992.792 kb on - strand at 2992.797 kb on + strand at 2992.816 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 2,990,658 + CA265_RS12070 0.82 +0.2 2,990,672 - CA265_RS12070 0.83 -3.0 2,990,796 + CA265_RS12070 0.87 -0.5 2,990,818 + CA265_RS12070 0.87 -0.2 2,990,894 - CA265_RS12070 0.90 -1.7 2,990,938 - +0.2 2,990,991 + +0.4 2,990,992 - -0.1 2,990,994 - +0.2 2,991,065 - +0.2 2,991,065 - +1.2 2,991,065 - +0.6 2,991,065 - +0.7 2,991,105 + -0.1 2,991,106 - +0.7 2,991,180 + +0.1 2,991,180 + +0.1 2,991,221 + +0.3 2,991,224 - +0.0 2,991,375 - -0.7 2,991,407 + +0.3 2,991,457 + -0.6 2,991,458 - -0.1 2,991,481 - -0.0 2,991,494 - -0.6 2,991,527 - +0.1 2,991,546 + +0.9 2,991,546 + +0.3 2,991,579 + +1.2 2,991,580 - -0.4 2,991,611 + -0.2 2,991,630 - -2.2 2,991,740 + CA265_RS12075 0.57 -0.5 2,991,741 - CA265_RS12075 0.57 -0.6 2,991,741 - CA265_RS12075 0.57 -0.1 2,991,744 + CA265_RS12075 0.59 -0.4 2,991,831 + -0.8 2,991,831 + -0.4 2,991,843 - -0.3 2,991,916 - -1.4 2,991,990 - +1.4 2,992,001 - -0.0 2,992,014 + -0.4 2,992,074 + -0.3 2,992,081 + -0.1 2,992,085 - +0.5 2,992,086 + +1.4 2,992,086 + +0.1 2,992,168 + -1.0 2,992,201 + -0.1 2,992,236 - -1.4 2,992,236 - +0.5 2,992,237 + -0.1 2,992,238 - -0.9 2,992,238 - +0.7 2,992,276 + CA265_RS12080 0.14 +1.0 2,992,326 - +0.2 2,992,329 - CA265_RS12080 0.23 -0.6 2,992,348 + CA265_RS12080 0.26 +0.2 2,992,349 - CA265_RS12080 0.26 +0.7 2,992,351 - CA265_RS12080 0.26 +0.3 2,992,351 - CA265_RS12080 0.26 +0.5 2,992,415 + CA265_RS12080 0.36 -0.7 2,992,415 + CA265_RS12080 0.36 -0.6 2,992,415 + CA265_RS12080 0.36 +0.0 2,992,474 + CA265_RS12080 0.46 +0.0 2,992,491 + CA265_RS12080 0.48 -1.2 2,992,492 - CA265_RS12080 0.48 -0.1 2,992,492 - CA265_RS12080 0.48 +0.6 2,992,576 - CA265_RS12080 0.62 +0.6 2,992,610 - CA265_RS12080 0.67 -1.6 2,992,633 + CA265_RS12080 0.71 +0.1 2,992,635 + CA265_RS12080 0.71 +0.3 2,992,636 - CA265_RS12080 0.71 +0.3 2,992,736 + CA265_RS12080 0.87 -0.3 2,992,736 + CA265_RS12080 0.87 +0.4 2,992,736 + CA265_RS12080 0.87 +0.3 2,992,736 + CA265_RS12080 0.87 -0.1 2,992,792 - -0.5 2,992,797 + +0.3 2,992,816 + -0.5
Or see this region's nucleotide sequence