Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1012 and Pf6N2E2_1013 are separated by 410 nucleotides
Pf6N2E2_1012 - hypothetical protein, at 1,182,300 to 1,182,518
_1012
Pf6N2E2_1013 - Enoyl-CoA hydratase (EC 4.2.1.17), at 1,182,929 to 1,183,753
_1013
Position (kb)
1182
1183 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1181.421 kb on - strand at 1181.460 kb on - strand at 1181.474 kb on + strand at 1181.474 kb on + strand at 1181.553 kb on - strand at 1181.796 kb on + strand at 1181.899 kb on + strand at 1182.119 kb on - strand at 1182.339 kb on + strand, within Pf6N2E2_1012 at 1182.386 kb on + strand, within Pf6N2E2_1012 at 1182.386 kb on + strand, within Pf6N2E2_1012 at 1182.442 kb on + strand, within Pf6N2E2_1012 at 1182.442 kb on + strand, within Pf6N2E2_1012 at 1182.498 kb on + strand at 1182.498 kb on + strand at 1182.498 kb on + strand at 1182.500 kb on + strand at 1182.500 kb on + strand at 1182.500 kb on + strand at 1182.501 kb on - strand at 1182.501 kb on - strand at 1182.501 kb on - strand at 1182.501 kb on - strand at 1182.501 kb on - strand at 1182.504 kb on + strand at 1182.505 kb on - strand at 1182.544 kb on + strand at 1182.709 kb on + strand at 1182.709 kb on + strand at 1182.709 kb on + strand at 1182.713 kb on + strand at 1182.861 kb on + strand at 1182.861 kb on + strand at 1182.861 kb on + strand at 1182.861 kb on + strand at 1182.896 kb on + strand at 1182.897 kb on - strand at 1182.899 kb on + strand at 1183.031 kb on + strand, within Pf6N2E2_1013 at 1183.031 kb on + strand, within Pf6N2E2_1013 at 1183.031 kb on + strand, within Pf6N2E2_1013 at 1183.032 kb on - strand, within Pf6N2E2_1013 at 1183.032 kb on - strand, within Pf6N2E2_1013 at 1183.057 kb on - strand, within Pf6N2E2_1013 at 1183.114 kb on + strand, within Pf6N2E2_1013 at 1183.114 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.145 kb on - strand, within Pf6N2E2_1013 at 1183.145 kb on - strand, within Pf6N2E2_1013 at 1183.152 kb on + strand, within Pf6N2E2_1013 at 1183.152 kb on + strand, within Pf6N2E2_1013 at 1183.153 kb on - strand, within Pf6N2E2_1013 at 1183.245 kb on + strand, within Pf6N2E2_1013 at 1183.245 kb on + strand, within Pf6N2E2_1013 at 1183.297 kb on - strand, within Pf6N2E2_1013 at 1183.439 kb on + strand, within Pf6N2E2_1013
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 1,181,421 - +0.0 1,181,460 - -0.9 1,181,474 + +0.9 1,181,474 + -1.7 1,181,553 - +1.0 1,181,796 + -0.3 1,181,899 + -0.1 1,182,119 - +0.0 1,182,339 + Pf6N2E2_1012 0.18 -0.1 1,182,386 + Pf6N2E2_1012 0.39 -0.0 1,182,386 + Pf6N2E2_1012 0.39 -1.1 1,182,442 + Pf6N2E2_1012 0.65 -0.5 1,182,442 + Pf6N2E2_1012 0.65 -0.4 1,182,498 + -0.2 1,182,498 + +0.1 1,182,498 + -0.1 1,182,500 + +0.4 1,182,500 + +0.4 1,182,500 + +0.7 1,182,501 - +2.6 1,182,501 - +0.8 1,182,501 - +0.1 1,182,501 - +1.9 1,182,501 - +0.6 1,182,504 + +0.1 1,182,505 - +0.7 1,182,544 + -0.3 1,182,709 + +0.4 1,182,709 + -0.1 1,182,709 + +1.0 1,182,713 + +0.1 1,182,861 + -0.3 1,182,861 + -1.8 1,182,861 + -0.6 1,182,861 + +0.3 1,182,896 + +0.8 1,182,897 - -1.3 1,182,899 + +1.2 1,183,031 + Pf6N2E2_1013 0.12 +1.0 1,183,031 + Pf6N2E2_1013 0.12 +1.4 1,183,031 + Pf6N2E2_1013 0.12 -0.5 1,183,032 - Pf6N2E2_1013 0.12 +0.4 1,183,032 - Pf6N2E2_1013 0.12 -1.3 1,183,057 - Pf6N2E2_1013 0.16 -0.6 1,183,114 + Pf6N2E2_1013 0.22 -0.2 1,183,114 + Pf6N2E2_1013 0.22 -0.4 1,183,144 + Pf6N2E2_1013 0.26 -0.6 1,183,144 + Pf6N2E2_1013 0.26 -1.3 1,183,144 + Pf6N2E2_1013 0.26 +1.1 1,183,144 + Pf6N2E2_1013 0.26 +1.0 1,183,144 + Pf6N2E2_1013 0.26 +0.1 1,183,144 + Pf6N2E2_1013 0.26 +0.3 1,183,144 + Pf6N2E2_1013 0.26 -0.4 1,183,144 + Pf6N2E2_1013 0.26 -0.6 1,183,144 + Pf6N2E2_1013 0.26 -0.1 1,183,145 - Pf6N2E2_1013 0.26 +1.0 1,183,145 - Pf6N2E2_1013 0.26 +0.1 1,183,152 + Pf6N2E2_1013 0.27 -1.7 1,183,152 + Pf6N2E2_1013 0.27 +0.1 1,183,153 - Pf6N2E2_1013 0.27 +0.7 1,183,245 + Pf6N2E2_1013 0.38 +0.4 1,183,245 + Pf6N2E2_1013 0.38 -0.2 1,183,297 - Pf6N2E2_1013 0.45 +0.1 1,183,439 + Pf6N2E2_1013 0.62 +0.4
Or see this region's nucleotide sequence