Experiment: A22 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybfD and ybgA are separated by 147 nucleotides ybgA and phr overlap by 4 nucleotides phr and ybgH are separated by 149 nucleotides
b0706: ybfD - hypothetical protein (NCBI), at 737,315 to 738,076
ybfD
b0707: ybgA - hypothetical protein (NCBI), at 738,224 to 738,733
ybgA
b0708: phr - deoxyribodipyrimidine photolyase, FAD-binding (NCBI), at 738,730 to 740,148
phr
b0709: ybgH - predicted transporter (NCBI), at 740,298 to 741,779
ybgH
Position (kb) 738
739
740
741 Strain fitness (log2 ratio) -2
-1
0
1
2 at 737.767 kb on + strand, within ybfD at 737.777 kb on + strand, within ybfD at 737.977 kb on - strand, within ybfD at 737.979 kb on - strand, within ybfD at 738.012 kb on - strand at 738.032 kb on - strand at 738.120 kb on - strand at 738.199 kb on - strand at 738.200 kb on + strand at 738.200 kb on + strand at 738.208 kb on - strand at 738.208 kb on - strand at 738.211 kb on + strand at 738.251 kb on + strand at 738.252 kb on + strand at 738.392 kb on - strand, within ybgA at 738.392 kb on - strand, within ybgA at 738.435 kb on + strand, within ybgA at 738.443 kb on - strand, within ybgA at 738.531 kb on - strand, within ybgA at 738.710 kb on - strand at 738.717 kb on + strand at 738.717 kb on + strand at 738.759 kb on + strand at 738.787 kb on - strand at 738.831 kb on + strand at 738.831 kb on + strand at 738.831 kb on + strand at 738.831 kb on + strand at 738.837 kb on + strand at 738.844 kb on - strand at 738.844 kb on - strand at 738.844 kb on - strand at 739.092 kb on + strand, within phr at 739.122 kb on - strand, within phr at 739.124 kb on + strand, within phr at 739.322 kb on - strand, within phr at 739.415 kb on + strand, within phr at 739.415 kb on + strand, within phr at 739.475 kb on - strand, within phr at 739.648 kb on + strand, within phr at 739.648 kb on + strand, within phr at 739.648 kb on - strand, within phr at 739.648 kb on - strand, within phr at 739.720 kb on + strand, within phr at 739.765 kb on - strand, within phr at 740.129 kb on - strand at 740.287 kb on + strand at 740.366 kb on + strand at 740.534 kb on - strand, within ybgH at 740.728 kb on - strand, within ybgH at 740.728 kb on - strand, within ybgH at 740.753 kb on + strand, within ybgH at 740.756 kb on - strand, within ybgH at 740.813 kb on + strand, within ybgH at 740.813 kb on + strand, within ybgH at 740.813 kb on + strand, within ybgH at 740.821 kb on - strand, within ybgH at 740.821 kb on - strand, within ybgH at 740.924 kb on + strand, within ybgH at 740.932 kb on - strand, within ybgH at 741.037 kb on - strand, within ybgH at 741.041 kb on - strand, within ybgH at 741.041 kb on - strand, within ybgH at 741.046 kb on - strand, within ybgH at 741.046 kb on - strand, within ybgH at 741.071 kb on - strand, within ybgH at 741.071 kb on - strand, within ybgH at 741.071 kb on - strand, within ybgH at 741.082 kb on + strand, within ybgH at 741.082 kb on + strand, within ybgH at 741.090 kb on - strand, within ybgH at 741.092 kb on + strand, within ybgH at 741.092 kb on + strand, within ybgH at 741.100 kb on - strand, within ybgH at 741.100 kb on - strand, within ybgH at 741.102 kb on + strand, within ybgH at 741.104 kb on - strand, within ybgH
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.08 mg/ml remove 737,767 + ybfD b0706 0.59 +0.1 737,777 + ybfD b0706 0.61 -0.3 737,977 - ybfD b0706 0.87 +1.6 737,979 - ybfD b0706 0.87 +1.5 738,012 - -0.3 738,032 - +0.7 738,120 - +2.3 738,199 - +0.2 738,200 + -0.3 738,200 + -2.6 738,208 - +1.0 738,208 - -0.6 738,211 + +0.6 738,251 + +0.6 738,252 + +1.2 738,392 - ybgA b0707 0.33 +0.7 738,392 - ybgA b0707 0.33 +0.3 738,435 + ybgA b0707 0.41 -0.5 738,443 - ybgA b0707 0.43 +0.7 738,531 - ybgA b0707 0.60 +2.1 738,710 - +0.3 738,717 + +1.3 738,717 + +0.4 738,759 + +0.8 738,787 - -1.3 738,831 + -0.8 738,831 + -0.6 738,831 + -0.3 738,831 + -1.6 738,837 + -0.3 738,844 - -0.8 738,844 - +0.4 738,844 - -0.3 739,092 + phr b0708 0.26 +1.0 739,122 - phr b0708 0.28 +0.5 739,124 + phr b0708 0.28 -0.8 739,322 - phr b0708 0.42 +1.7 739,415 + phr b0708 0.48 +0.7 739,415 + phr b0708 0.48 +2.2 739,475 - phr b0708 0.53 +0.7 739,648 + phr b0708 0.65 -0.3 739,648 + phr b0708 0.65 -0.8 739,648 - phr b0708 0.65 +2.3 739,648 - phr b0708 0.65 +0.7 739,720 + phr b0708 0.70 +0.6 739,765 - phr b0708 0.73 +0.4 740,129 - -1.3 740,287 + -0.3 740,366 + +0.7 740,534 - ybgH b0709 0.16 -0.8 740,728 - ybgH b0709 0.29 -1.0 740,728 - ybgH b0709 0.29 +1.7 740,753 + ybgH b0709 0.31 -0.8 740,756 - ybgH b0709 0.31 -0.3 740,813 + ybgH b0709 0.35 -0.5 740,813 + ybgH b0709 0.35 -0.7 740,813 + ybgH b0709 0.35 +1.6 740,821 - ybgH b0709 0.35 +0.3 740,821 - ybgH b0709 0.35 +0.3 740,924 + ybgH b0709 0.42 -0.3 740,932 - ybgH b0709 0.43 +0.7 741,037 - ybgH b0709 0.50 -0.6 741,041 - ybgH b0709 0.50 -0.3 741,041 - ybgH b0709 0.50 +2.7 741,046 - ybgH b0709 0.50 -1.0 741,046 - ybgH b0709 0.50 -0.4 741,071 - ybgH b0709 0.52 +0.6 741,071 - ybgH b0709 0.52 +1.7 741,071 - ybgH b0709 0.52 +0.2 741,082 + ybgH b0709 0.53 +2.7 741,082 + ybgH b0709 0.53 -0.3 741,090 - ybgH b0709 0.53 +0.9 741,092 + ybgH b0709 0.54 +0.2 741,092 + ybgH b0709 0.54 +0.6 741,100 - ybgH b0709 0.54 +0.4 741,100 - ybgH b0709 0.54 -0.8 741,102 + ybgH b0709 0.54 -0.3 741,104 - ybgH b0709 0.54 +0.3
Or see this region's nucleotide sequence