Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_R0032 and Shewana3_R0033 are separated by 47 nucleotides Shewana3_R0033 and Shewana3_R0034 are separated by 22 nucleotides Shewana3_R0034 and Shewana3_R0035 are separated by 1 nucleotides Shewana3_R0035 and Shewana3_R0036 are separated by 83 nucleotides Shewana3_R0036 and Shewana3_R0037 are separated by 36 nucleotides Shewana3_R0037 and Shewana3_1013 are separated by 288 nucleotides Shewana3_1013 and Shewana3_1014 are separated by 483 nucleotides
Shewana3_R0032: Shewana3_R0032 - tRNA-Leu (RefSeq), at 1,184,307 to 1,184,391
_R0032
Shewana3_R0033: Shewana3_R0033 - tRNA-Gln (RefSeq), at 1,184,439 to 1,184,513
_R0033
Shewana3_R0034: Shewana3_R0034 - tRNA-Gln (RefSeq), at 1,184,536 to 1,184,610
_R0034
Shewana3_R0035: Shewana3_R0035 - tRNA-Met (RefSeq), at 1,184,612 to 1,184,688
_R0035
Shewana3_R0036: Shewana3_R0036 - tRNA-Leu (RefSeq), at 1,184,772 to 1,184,856
_R0036
Shewana3_R0037: Shewana3_R0037 - tRNA-Gln (RefSeq), at 1,184,893 to 1,184,967
_R0037
Shewana3_1013: Shewana3_1013 - hypothetical protein (RefSeq), at 1,185,256 to 1,185,891
_1013
Shewana3_1014: Shewana3_1014 - hypothetical protein (RefSeq), at 1,186,375 to 1,186,908
_1014
Position (kb)
1185
1186 Strain fitness (log2 ratio)
-2
-1
0
1 at 1185.324 kb on - strand, within Shewana3_1013 at 1185.393 kb on - strand, within Shewana3_1013 at 1185.623 kb on - strand, within Shewana3_1013 at 1185.863 kb on - strand at 1185.911 kb on - strand at 1185.943 kb on - strand at 1186.031 kb on + strand at 1186.420 kb on + strand at 1186.520 kb on - strand, within Shewana3_1014 at 1186.646 kb on + strand, within Shewana3_1014 at 1186.646 kb on + strand, within Shewana3_1014 at 1186.654 kb on - strand, within Shewana3_1014 at 1186.654 kb on - strand, within Shewana3_1014 at 1186.707 kb on + strand, within Shewana3_1014 at 1186.748 kb on + strand, within Shewana3_1014 at 1186.890 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 1,185,324 - Shewana3_1013 0.11 -0.1 1,185,393 - Shewana3_1013 0.22 +0.7 1,185,623 - Shewana3_1013 0.58 +1.4 1,185,863 - -1.4 1,185,911 - -0.0 1,185,943 - -0.1 1,186,031 + -2.0 1,186,420 + +0.5 1,186,520 - Shewana3_1014 0.27 +0.7 1,186,646 + Shewana3_1014 0.51 +0.4 1,186,646 + Shewana3_1014 0.51 -0.2 1,186,654 - Shewana3_1014 0.52 +0.5 1,186,654 - Shewana3_1014 0.52 +0.5 1,186,707 + Shewana3_1014 0.62 -0.9 1,186,748 + Shewana3_1014 0.70 +0.7 1,186,890 + +0.3
Or see this region's nucleotide sequence